Identification of water pathogens by Raman microspectroscopy

Kusić, Dragana; Kampe, Bernd; Rösch, Petra; Popp, Jürgen

doi:10.1016/j.watres.2013.09.030

Cited by 84 publications

(75 citation statements)

References 47 publications

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“…[1][2][3][4][5][6][7][8][9][10][11][12] In vitro measurement requires placing the biological specimen on a substrate as a sample holder. However, the Raman spectra of the sample holder itself need to be dormant so that no Raman spectral signature of the holder itself is interfering with the sample's Raman signals.…”

Section: Introductionmentioning

confidence: 99%

Characterization of different substrates for Raman spectroscopic imaging of eukaryotic cells

Ramoji

Galler

Gläser

et al. 2016

J Raman Spectroscopy

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For Raman spectroscopic analyses of the cells and other biological samples, the choice of the right substrate material is very important to avoid loss of information in characteristic spectral features because of competing background signals. In the current study, Raman spectroscopy is used to characterize several potential Raman substrates. Raman vibrational bands of the substrate material are discussed. The surface topography is analyzed by atomic force microscopy, and the root mean square surface roughness values are reported. Biocompatibility of the substrates is tested with Hep G2 cells evaluating cellular morphology as well as live/dead staining. Calcium fluoride, silicon, fused silica, borofloat glass, and silicon nitride membranes support cell growth and adherence. Silicon, borofloat glass, and fused silica give rise to Raman signals in the region of interest. Calcium fluoride substrate (UV grade) is suitable for Raman spectroscopic investigation of living cells. Nickel foil is suitable substrate for Raman spectroscopic investigation but cellular adherence and viability depend on the quality of the foil. Silicon nitride membranes coated with nickel chrome is a suitable Raman substrate in closed microfluidic systems.

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Section: Introductionmentioning

confidence: 99%

Characterization of different substrates for Raman spectroscopic imaging of eukaryotic cells

Ramoji

Galler

Gläser

et al. 2016

J Raman Spectroscopy

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“…Components such as DNA, RNA, proteins, lipids, and carbohydrates of the bacterial cells may vary and, therefore, cause the Raman spectra of two different species to be slightly different which can be successfully used for species discrimination [17,18]. However, to be able to differentiate between the Raman spectra of diverse bacterial species, multivariate analysis has to be applied to the data [19].…”

Section: Introductionmentioning

confidence: 99%

“…In the work described here, Raman microspectroscopy has been used to evaluate the change in physiology due to a lack of nutrients by comparing the Raman spectra of bacteria that were grown in tap water with the Raman spectra of those grown on agar media [17]. Also, Raman microspectroscopy has been used to investigate differences in the chemical composition of both planktonic form and sessile cells of singlespecies biofilms of six strains of Legionella (Legionella bozemanae, Legionella micdadei, Legionella feeleii, Legionella pneumophila ssp.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopic differentiation of planktonic bacteria and biofilms

et al. 2015

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Both biofilm formations as well as planktonic cells of water bacteria such as diverse species of the Legionella genus as well as Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli were examined in detail by Raman microspectroscopy. Production of various molecules involved in biofilm formation of tested species in nutrient-deficient media such as tap water was observed and was particularly evident in the biofilms formed by six Legionella species. Biofilms of selected species of the Legionella genus differ significantly from the planktonic cells of the same organisms in their lipid amount. Also, all Legionella species have formed biofilms that differ significantly from the biofilms of the other tested genera in the amount of lipids they produced. We believe that the significant increase in the synthesis of this molecular species may be associated with the ability of Legionella species to form biofilms. In addition, a combination of Raman microspectroscopy with chemometric approaches can distinguish between both planktonic form and biofilms of diverse bacteria and could be used to identify samples which were unknown to the identification model. Our results provide valuable data for the development of fast and reliable analytic methods based on Raman microspectroscopy, which can be applied to the analysis of tap water-adapted microorganisms without any cultivation step.

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“…It has several applications in chemistry, physics and biology, highlighting studies on the composition of materials in different areas, such as microbiology, arts, archaeology, geology and atmospheric sciences, among many others [17][18][19][20][21][22][23][24]. Since handheld Raman spectrometers with short acquisition times and reasonable spectral resolutions are available, the applicability of this technique in industry has remarkably increased.…”

Section: Introductionmentioning

confidence: 99%

Handheld Raman Spectroscopy for the Distinction of Essential Oils Used in the Cosmetics Industry

Jentzsch

Ramos²,

Ciobotă³

2015

Cosmetics

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Essential oils are highly appreciated by the cosmetics industry because they have antimicrobial and antioxidant properties, among others. Since essential oils are natural products, their inclusion in cosmetic formulations is a common practice. Currently, low-quality and/or adulterated essential oils can be found on the market; therefore, analytical methods for control are required. Raman spectroscopy is a versatile technique that can be used for quality control tasks; the portability of modern devices expand the analytical possibilities also to in situ measurements. Fifteen essential oils of interest for the cosmetics industry were measured using a handheld Raman spectrometer, and the assignment of the main bands observed in their average spectra was proposed. In most cases, it is possible to distinguish the essential oils by a simple visual inspection of their characteristic Raman bands. However, for essential oils extracted from closely-related vegetable species and containing the same main component in a very high proportion, the visual inspection of the spectra may be not enough, and the application of chemometric methods is suggested. Characteristic Raman bands for each essential oil can be used to both identify the essential oils and detect adulterations. OPEN ACCESSCosmetics 2015, 2 163

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Identification of water pathogens by Raman microspectroscopy

Cited by 84 publications

References 47 publications

Characterization of different substrates for Raman spectroscopic imaging of eukaryotic cells

Characterization of different substrates for Raman spectroscopic imaging of eukaryotic cells

Raman spectroscopic differentiation of planktonic bacteria and biofilms

Handheld Raman Spectroscopy for the Distinction of Essential Oils Used in the Cosmetics Industry

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