Handheld Raman Spectroscopy for the Distinction of Essential Oils Used in the Cosmetics Industry

Jentzsch, Paul Vargas; Ramos, Luis A.; Ciobotă, Valerian

doi:10.3390/cosmetics2020162

Cited by 53 publications

(49 citation statements)

References 54 publications

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“…The band at 2870 cm −1 is assigned to terpenoid vibrations of the PEP (Schulz et al, 2004). The other strong peaks at 1457 and 769 cm −1 correspond to CH 3 /CH 2 bending of menthol and the ring deformation mode of menthol, respectively (Rösch et al, 2002;Jentzsch et al, 2015). As presented in Figure 3b, the typical peaks of PCL at 2916 and 2864 cm −1 can be related to the asymmetric and symmetric vibrations of the CH 2 group.…”

Section: Raman Spectroscopy Analysismentioning

confidence: 89%

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Ünalan

Slavik

Buettner

et al. 2019

Front. Bioeng. Biotechnol.

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The aim of this study was to fabricate and characterize various concentrations of peppermint essential oil (PEP) loaded on poly(ε-caprolactone) (PCL) electrospun fiber mats for healing applications, where PEP was intended to impart antibacterial activity to the fibers. SEM images illustrated that the morphology of all electrospun fiber mats was smooth, uniform, and bead-free. The average fiber diameter was reduced by the addition of PEP from 1.6 ± 0.1 to 1.0 ± 0.2 µm. Functional groups of the fibers were determined by Raman spectroscopy. Gas chromatography-mass spectroscopy (GC-MS) analysis demonstrated the actual PEP content in the samples. In vitro degradation was determined by measuring weight loss and their morphology change, showing that the electrospun fibers slightly degraded by the addition of PEP. The wettability of PCL and PEP loaded electrospun fiber mats was measured by determining contact angle and it was shown that wettability increased with the incorporation of PEP. The antimicrobial activity results revealed that PEP loaded PCL electrospun fiber mats exhibited inhibition against Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria. In addition, an in-vitro cell viability assay using normal human dermal fibroblast (NHDF) cells revealed improved cell viability on PCL, PCLPEP1.5, PCLPEP3, and PCLGEL6 electrospun fiber mats compared to the control (CNT) after 48 h cell culture. Our findings showed for the first time PEP loaded PCL electrospun fiber mats with antibiotic-free antibacterial activity as promising candidates for wound healing applications.

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Section: Raman Spectroscopy Analysismentioning

confidence: 89%

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Ünalan

Slavik

Buettner

et al. 2019

Front. Bioeng. Biotechnol.

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“…42 Moreover, the bands at 1452, 1413 and 1378 cm −1 are attributed to CH 3 and CH 2 ; while, the peak at 1298 cm −1 is assigned to the =CH. 43 The spectrum of the composite SA-PEO/LO fibers (violet curve in Fig. 2) shows the Raman peaks of all the constituent materials (SA, PEO, and LO), pointing out that the essential oil and particularly its active compounds were well incorporated into the fibers.…”

Section: Physicochemical Properties Of the Nanofibersmentioning

confidence: 99%

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

et al. 2016

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This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication.Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available.You can find more information about Accepted Manuscripts in the Information for Authors. One of the current challenges in wound care is the development of multifunctional dressings that can both protect the wound from external agents and promote the regeneration of the new tissue. Here, we show the combined use of two naturally derived compounds, sodium alginate and lavender essential oil, for the production of bioactive nanofibrous dressings by electrospinning, and their efficacy for the treatment of skin burns induced by midrange ultraviolet radiation (UVB). We demonstrate that the engineered dressings reduce the risk of microbial infection of the burn, since they stop the growth of Staphylococcus aureus. Furthermore, they are able to control and reduce the inflammatory response that is induced in human foreskin fibroblasts by lipopolysaccharides, and in rodents by UVB exposure. In particular, we report a remarkable reduction of pro-inflammatory cytokines when fibroblasts or animals are treated with the alginate-based nanofibers. The down-regulation of cytokines production and the absence of erythema on the skin of the treated animals confirm that the here described dressings are promising as advanced biomedical devices for burn management.

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“…The oils are Raman-active substances [20,31]. Therefore, the study reported herein aimed at investigating the penetration profiles of different oils in the human and porcine skin (in vivo and ex vivo) and evaluating their influences on the stratum corneum by means of CRM.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Human and Porcine Skin in vivo/ex vivo for Penetration of Selected Oils by Confocal Raman Microscopy

Choe

Lademann

Darvin

2015

Skin Pharmacol Physiol

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Background: The subject of oil penetration into the skin is controversially discussed in the scientific literature. Methods: Confocal Raman microscopy was used for analyzing oil penetration into the skin. The following methods were applied in the study: methods based on tracking specific peaks (method 1), the nonrestricted multiple least square fit (method 2), analyzing the lipid-to-keratin peak ratio using the perpendicular drop-down cutoff procedure (method 3), and the Gaussian function-based deconvolution procedure (method 4). Results: The results obtained using methods 1, 2 and 4 show that the investigated oils do not penetrate deeper than 11 µm into human and porcine skin. Petrolatum has a prominent swelling effect on the stratum corneum (32% in vivo, 28% ex vivo), while the other oils exhibit no significant swelling effect. By using method 3, the penetration profile of oils, and especially of petrolatum, into the skin was interpreted incorrectly for various reasons that are addressed herein below. Conclusion: Predominantly remaining in the uppermost corneocyte layers of the stratum corneum, topically applied oils do not reach the viable cells of the stratum spinosum. To exclude any possible mistakes when using the lipid-keratin Raman peak (2,820-3,030 cm^-1), the penetration analysis should be performed using the Gaussian function-based deconvolution procedure.

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Handheld Raman Spectroscopy for the Distinction of Essential Oils Used in the Cosmetics Industry

Cited by 53 publications

References 54 publications

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing

Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

Analysis of Human and Porcine Skin in vivo/ex vivo for Penetration of Selected Oils by Confocal Raman Microscopy

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