Identification of Tropical Plant Extracts That Extend Yeast Chronological Life Span

Kwong, Mandy; Lee, Jee Whu; Samian, Mohammed Razip; Wahab, Habibah A.; Watanabe, Nobumoto; Ong, Eugene Boon Beng

doi:10.3390/cells10102718

Cited by 4 publications

(3 citation statements)

References 44 publications

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“…The behaviour of this extract showed that the sensitivity of the extract caused inhibition in cell viability to occur at physiologically relevant concentrations (low concentrations) and increasing concentrations resulted in no further inhibition in cell viability. This effect is consistent with previous studies where similar effects have been reported [ 41 , 42 ].…”

Section: Discussionsupporting

confidence: 94%

Antiproliferative activities of some selected Nigerian medicinal plants against breast, liver, and cervical cancer cells

Olaleye,

Kim,

Spriggs

2024

BMC Complement Med Ther

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Background Phytochemicals have become a growing source of alternative medicine in developing countries due to the poor prognosis, high cost of conventional pharmaceuticals, and undesirable effects associated with mainstream cancer treatment. Objective This study was aimed at investigating the anticancer effect of some selected Nigerian medicinal plants used in cancer treatment. These include ethanol extracts of Dialium guineense root (DGR), Dialium guineense leaves (DGL), Jateorhiza macrantha leaves (JML), Musanga cecropioides leaves (MCL), Musanga cecropioides stembark (MCSB), Piptadeniastrum africanum stembark (PASB), Piptadeniastrum africanum root (PAR), Pupalia lappacea flower tops (PLF), Raphiostylis beninensis root (RBR), Raphiostylis beninensis leaves (RBL), Ritchiea capparoides leaves (RCL), Ritchiea capparoides stembark (RCSB), and Triplochiton scleroxylon stembark (TSB). Methods The cytotoxic activity of the extracts was examined using a brine shrimp lethality assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against three cancer cell lines, including MCF-7, HUH-7, and HeLa. The selectivity of all extracts towards cancer cells was investigated using normal lung fibroblasts (MRC-5). Cell migration and colony-forming assays of active extracts against MCF-7 cells were also performed. Additionally, the total polyphenolic contents of the active extracts were estimated using standard methods. Results The extract of PASB had the highest cytotoxicity (LC50 = 1.58 μg/mL) on the brine shrimps compared to vincristine sulphate (LC50 = 2.24 μg/mL). In the cell viability assay, all the extracts produced significant (p < 0.05) growth inhibitory effects against all cell lines tested in a dose-dependent manner. All extracts were selective to cancer cells at varying degrees. Worth mentioning are the extracts of MCL, DGR, RBR, and PASB, which exhibited 14-, 7-, 6- and 2-fold selectivity toward MCF-7 cancer cells relative to normal lung fibroblast (MRC-5), respectively. These four extracts also significantly inhibited cell migration and colony formation in MCF-7-treated cells in dose-dependent manners. Considerable amounts of phenolics, flavonoids, and proanthocyanidins were detected in all extracts evaluated. Conclusion These findings advocate the continued development of MCL, DGR, RBR, and PASB as potential chemotherapeutic agents.

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Section: Discussionsupporting

confidence: 94%

Antiproliferative activities of some selected Nigerian medicinal plants against breast, liver, and cervical cancer cells

Olaleye,

Kim,

Spriggs

2024

BMC Complement Med Ther

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“…Doubling times (δ) were calculated between every consecutive pair of absorbance values, by the formula: δ = , whereby A 1 and A 2 are successive absorbance values while t 1 and t 2 are the times between measurement of successive absorbance. Survival percentage of each replicate of each strain was then averaged 1 , 36 .…”

Section: Methodsmentioning

confidence: 99%

Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

Lee

Ong

Samian

et al. 2021

Sci Rep

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Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been identified, however the functions of most of these ageing-related proteins are not known. Here, we report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. Three of the CLS-extending proteins, Ptc4, Zwf1, and Sme1, contributed to an overall higher survival percentage and shorter doubling time of yeast growth compared to the control. The CLS-extending proteins contributed to thermal and oxidative stress responses differently, suggesting different mechanisms of actions. The overexpression of Ptc4 or Zwf1 also promoted rapid cell proliferation during yeast growth, suggesting their involvement in cell division or growth pathways.

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“…In the second approach, the so-called chronological aging is used as a model for studying postmitotic cells of higher eukaryotes, including humans [16,20]. Many factors are known to influence the aging of yeast, including calory restriction [21], anti-aging compounds [22][23][24], ribosome biogenesis disorders [25][26][27], mitochondrial dysfunction [28], and an increased level of reactive oxygen species, leading to oxidative damage [29] or extrachromosomal rDNA circles (ERCs) formation [30,31]. However, despite extensive research on aging, little is known about the possible linkage between replication initiation and aging.…”

Section: Introductionmentioning

confidence: 99%

Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast

Stępień

Skoneczna

Kula-Maximenko

et al. 2022

Cells

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Precise DNA replication is pivotal for ensuring the accurate inheritance of genetic information. To avoid genetic instability, each DNA fragment needs to be amplified only once per cell cycle. DNA replication in eukaryotes starts with the binding of the origin recognition complex (ORC) to the origins of DNA replication. The genes encoding ORC subunits have been conserved across eukaryotic evolution and are essential for the initiation of DNA replication. In this study, we conducted an extensive physiological and aging-dependent analysis of heterozygous cells lacking one copy of ORC genes in the BY4743 background. Cells with only one copy of the ORC genes showed a significant decrease in the level of ORC mRNA, a delay in the G1 phase of the cell cycle, and an extended doubling time. Here, we also show that the reducing the levels of Orc1-6 proteins significantly extends both the budding and average chronological lifespans. Heterozygous ORC/orcΔ and wild-type diploid cells easily undergo haploidization during chronological aging. This ploidy shift might be related to nutrient starvation or the inability to survive under stress conditions. A Raman spectroscopy analysis helped us to strengthen the hypothesis of the importance of lipid metabolism and homeostasis in aging.

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Identification of Tropical Plant Extracts That Extend Yeast Chronological Life Span

Cited by 4 publications

References 44 publications

Antiproliferative activities of some selected Nigerian medicinal plants against breast, liver, and cervical cancer cells

Antiproliferative activities of some selected Nigerian medicinal plants against breast, liver, and cervical cancer cells

Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

Depletion of the Origin Recognition Complex Subunits Delays Aging in Budding Yeast

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