Screening of selected ageing-related proteins that extend chronological life span in yeast Saccharomyces cerevisiae

Abstract

Ageing-related proteins play various roles such as regulating cellular ageing, countering oxidative stress, and modulating signal transduction pathways amongst many others. Hundreds of ageing-related proteins have been identified, however the functions of most of these ageing-related proteins are not known. Here, we report the identification of proteins that extended yeast chronological life span (CLS) from a screen of ageing-related proteins. Three of the CLS-extending proteins, Ptc4, Zwf1, and Sme1, contributed to an overall higher survival percentage and shorter doubling time of yeast growth compared to the control. The CLS-extending proteins contributed to thermal and oxidative stress responses differently, suggesting different mechanisms of actions. The overexpression of Ptc4 or Zwf1 also promoted rapid cell proliferation during yeast growth, suggesting their involvement in cell division or growth pathways.

Studying the impact of cell age on the yeast growth behaviour of Saccharomyces pastorianus var. carlsbergensis by magnetic separation

“…In contrast, chronological lifespan is related to the length of time a non‐dividing cell can maintain its viability and re‐enter proliferation under favourable conditions. [ 22 ] Metabolism might be of key importance to the molecular causes of chronological aging. Studies have confirmed that ethanol plays a vital role in aging, since ethanol influences the respiratory chain and, in turn, the chronological aging process, [ 23 ] whereas acidification due to acetic acid has been put forward as the determinant cause of aging.…”