Identification of the stem rust resistance gene <i>Pg9</i> and its association with crown rust resistance and endosperm proteins in 'Dumont' oat

Chong, J.; Howes, N. K.; Brown, P. D.; Harder, D. E.

doi:10.1139/g94-062

Cited by 37 publications

(22 citation statements)

References 16 publications

(25 reference statements)

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“…The clustering of oat R genes conferring resistance to different races of a pathogen has been well documented by genetic analysis (Marshall and Shaner 1992;Sebesta et al 2000). Close linkage between the genes conferring resistance to the two main fungal diseases of oats has also been observed (Chong et al 1994). At the molecular level, individually cloned genes belonging to the same cluster encode different resistance specificities for the same pathogen, as in locus Mla for powdery mildew resistance in barley (Halterman et al 2001).…”

Section: Association Of Rgas With Disease Resistance Locimentioning

confidence: 95%

Isolation and mapping of resistance gene analogs from the Avena strigosa genome

et al. 2004

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Degenerate primers based on conserved regions of the nucleotide binding site (NBS) domain (encoded by the largest group of cloned plant disease resistance genes) were used to isolate a set of 15 resistance gene analogs (RGA) from the diploid species Avena strigosa Schreb. These were grouped into seven classes on the basis of 60% or greater nucleic acid sequence identity. Representative clones were used for genetic mapping in diploid and hexaploid oats. Two RGAs were mapped at two loci of the linkage group AswBF belonging to the A. strigosa x A. wiestii Steud map, and ten RGAs were mapped at 15 loci in eight linkage groups belonging to the A. byzantina C. Koch cv. Kanota x A. sativa L. cv. Ogle map. A similar approach was used for targeting genes encoding receptor-like kinases. Three different sequences were obtained and mapped to two linkage groups of the hexaploid oat map. Associations were explored between already known disease resistance loci mapped in different populations and the RGAs. Molecular markers previously linked to crown rust and barley yellow dwarf resistance genes or quantitative trait loci were found in the Kanota x Ogle map linked to RGAs at a distance ranging from 0 cM to 20 cM. Homoeologous RGAs were found to be linked to loci either conferring resistance to different isolates of the same pathogen or to different pathogens. This suggests that these RGAs identify genome regions containing resistance gene clusters.

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Section: Association Of Rgas With Disease Resistance Locimentioning

confidence: 95%

Isolation and mapping of resistance gene analogs from the Avena strigosa genome

et al. 2004

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“…On the other hand, because a number of crown rust resistance genes are found clustered in the oat genome (Chong et al 1994), the development of molecular markers to one crown rust resistance gene may be useful in the study of other resistance genes located within the same cluster. The availability of genetic markers for oat is limited because the large hexaploid genome of oat (AACCDD) presents extensive gene duplication.…”

Section: Introductionmentioning

confidence: 99%

“…A random amplified polymorphic DNA (RAPD) marker was obtained for Pc68 in a F 3 derived from the lines 'Makuru'*2//CAV4904/ 2*'SunII' (with Pc68) and 'Rodney O' (without Pc68), and this marker was associated with repulsion in the gene. Other molecular markers such as a sequence characterized amplified region (SCAR) (Koeyer et al 2000) and single nucleotide polymorphisms (SNPs) (Chen et al 2006) for the Pc68 gene were identified, considering that Pc68 is clustered with Pg9, a resistance gene to stem rust (Chong et al 1994). However, because the utility of any molecular marker is dependent on the polymorphism between the compared genotypes, it is unlikely that one single marker will be applicable across all oat germplasms, unless it differentiates the specific alleles that define the trait.…”

Section: Introductionmentioning

confidence: 99%

Molecular mapping of Pc68, a crown rust resistance gene in Avena sativa

et al. 2010

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Crown rust, which is caused by Puccinia coronata f. sp. avenae, P. Syd. & Syd., is the most destructive disease of cultivated oats (Avena sativa L.) throughout the world. Resistance to the disease that is based on a single gene is often short-lived because of the extremely great genetic diversity of P. coronata, which suggests that there is a need to develop oat cultivars with several resistance genes. This study aimed to identify amplified fragment length polymorphism AFLP markers that are linked to the major resistance gene, Pc68, and to amplify the F 6 genetic map from Pc68/5*Starter 9 UFRGS8. Seventy-eight markers with normal segregation were discovered and distributed in 12 linkage groups. The map covered 409.4 cM of the Avena sativa genome. Two AFLP markers were linked in repulsion to Pc68: U8PM22 and U8PM25, which flank the gene at 18.60 and 18.83 centiMorgans (cM), respectively. The marker U8PM25 is located in the linkage group 4_12 in the Kanota 9 Ogle reference oat population. These markers should be useful for transferring Pc68 to genotypes with good agronomic characteristics and for pyramiding crown rust resistance genes.

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“…CHONG & BROWN (1996) mostraram que a resistência à ferrugem da folha existente no acesso de aveia MG 85039 é determinada por dois genes complementares dominantes e que a existente no acesso MG 685181 é determinada por um único gene dominante, sendo que este gene tem mostrado grande utilidade como fonte de resistência no Canadá é foi designado Pc96. Em um estudo desenvolvido no Canadá, foi observado que a cultivar Dumont apresenta um terceiro gene de resistência à ferrugem da folha, uma vez que anteriormente já se sabia que a cultivar possuía os genes de resistência designados de Pc38 e Pc39, o terceiro gene detectado foi denominado de Pcx e se mostrou ligado ao gene de resistência à ferrugem do colmo Pg 9 (CHONG et al 1994). Entretanto em trigo, FENG et al (2004) relataram a ocorrência de genes de resistência recessivos ao fungo Stagonospora nodorum, em genótipos de trigo.…”

Section: Resultsunclassified

Herança da resistência à ferrugem da folha da aveia (Puccinia coronata f. sp. avenae Fraser & Led.) em genótipos brasileiros de aveia branca

et al. 2006

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RESUMO A ferrugem da folha da aveia é a moléstia mais importante que ataca a cultura da aveia, ocorrendo em praticamente todas as áreas em que a aveia é cultivada. A forma mais indicada para o seu controle é a utilização i) URPEL 15 (R) x UFRGS 7 (S), UPF 16 (R) x UFRGS 7 (S) e URPEL 15 (R) x UPF 16 (R), para a determinação da herança da resistência ao isolado um (1); ii) URPEL 15 (R) x UFRGS 7 (S), UPF 18 (R) x UFRGS 7 (S) e URPEL 15 (R) x UPF 18 (R), para a determinação da herança da resistência ao isolado dois (2); iii) URPEL 15 (R) x UFRGS 7 (S) e URPEL 15 (R) x UPF 18 (S), para a determinação da herança da resistência ao isolado três (3). Os resultados obtidos ABSTRACT Oat crown rust is the most important disease for the oat crop, occurring in practically all the areas where oat is cultivated. The most indicated form of control for this disease is the use of resistant cultivars. However, for the durable resistance to be acquired, it is necessary to know the genetics of resistance to crown rust in oats. Thus, the objective of this work was to determine the type of inheritance of resistance to three Puccinia coronata f. sp. avenae Fraser & Led., isolates (collected in southern Brazil) in brazilian white oat genotypes. To determine the inheritance of resistance to each one of three isolates,F 2 populations were used generated through artificial crosses, between resistant (R) and susceptible (S) and between resistant genotypes (R). Thus, F 2 populations from the following artificial crosses: i) URPEL 15 (R) x UFRGS 7 (S), UPF 16 (R) x UFRGS 7 (S) and URPEL 15 (R) x UPF 16 (R), were used to determine the inheritance of resistance to isolate one (1); ii) URPEL 15 (R) x UFRGS 7 (S), UPF 18 (R) x UFRGS 7 (S) and URPEL 15 (R) x UPF 18 (R), to determine the inheritance of resistance to isolate two (2); iii) URPEL 15 (R) x UFRGS 7 (S) and URPEL 15 (R) x UPF 18 (S), to determine the inheritance of resistance to isolate three (3).

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Identification of the stem rust resistance gene Pg9 and its association with crown rust resistance and endosperm proteins in 'Dumont' oat

Cited by 37 publications

References 16 publications

Isolation and mapping of resistance gene analogs from the Avena strigosa genome

Isolation and mapping of resistance gene analogs from the Avena strigosa genome

Molecular mapping of Pc68, a crown rust resistance gene in Avena sativa

Herança da resistência à ferrugem da folha da aveia (Puccinia coronata f. sp. avenae Fraser & Led.) em genótipos brasileiros de aveia branca

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