Identification of the Envelope Surface Glycoproteins of Equine Herpesvirus Type 1

The Nucleotide Sequence of an Equine Herpesvirus 4 Gene Homologue of the Herpes Simplex Virus 1 Glycoprotein H Gene

“…Electrophoretic analysis, by SDS-PAGE, of carbohydrate-containing envelope proteins of EHV-1 and EHV-4 has indicated that virions possess at least 12 glycoprotein species, eight of which are highly abundant within the virus envelope (Turtinen & Allen, 1982). It is apparent from recent studies on the nature of these glycoprotein species that some may be specified by the same gene, differing from each other only in the degree of their post-translational processing (Meredith et al, 1989).…”

“…It remains to be determined whether the native, processed EHV-4 gH corresponds to any of the previously reported EHV-4 glycoproteins (Turtinen & Allen, 1982;Meredith et al, 1989;Bridges et al, 1988). The predicted polypeptide has the potential to exhibit the following structural features characteristic of membrane glycoproteins.…”

Transcript Analysis of The Equine Herpesvirus 1 Glycoprotein B Gene Homologue and Its Expression by a Recombinant Vaccinia Virus

“…EHV-1 synthesizes several major and minor glycoprotein antigens which are localized both in the envelope of the virus and in the plasma membrane of infected cells Turtinen & Allen, 1982). Recently, several glycoprotein genes of both EHV-1 and the closely related EHV-4 have been identified and sequenced, namely the gC homologue (gpl3) and the gB homologue (gpl4) (Allen & Coogle, 1988;Whalley et al, 1989;Riggio et al, 1989).…”

Equine Herpesvirus Type 1 Unique Short Fragment Encodes Glycoproteins with Homology to Herpes Simplex Virus Type 1 gD, gI and gE

“…EHV-1 and EHV-4, although cross-neutralizing, can be distinguished by their antigenicity profiles, restriction endonuclease fingerprints and pathogenic properties in horses ). Both express six major glycoproteins designated gp2, gpl0, gpl3, gpl4, gp17/18 and gp21/22 with Mr values of 200K, 125K, 95K, 90K, 68K and 45K, respectively, and at least five minor glycoproteins (Turtinen & Allen, 1982). The genomic portions of the DNA sequence encoding the six major EHV-1 glycoproteins have been identified by using 2gt 11 expression libraries and monoclonal antibodies (MAbs) specific to the individual glycoproteins (Allen & Yeargan, 1987).…”