The Nucleotide Sequence of an Equine Herpesvirus 4 Gene Homologue of the Herpes Simplex Virus 1 Glycoprotein H Gene

Nicolson, Lesley; Cullinane, Ann; Onions, David

doi:10.1099/0022-1317-71-8-1793

Cited by 38 publications

(24 citation statements)

References 39 publications

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“…The presence of DNA sequence motifs identical to that recognized by the HSV-1 UL9 protein either adjacent to, or within, A + T-rich palindromes is a feature shared by known or presumed replication origins of other members of the alphaherpesvirus subfamily (Stow & Davison, 1986;Baumann et al, 1989;Nicolson et al, 1990;Robertson et al, 1991 ;Klupp et al, 1992). In the case of varicella-zoster virus (VZV) and equine herpesvirus 1 (EHV-1), DNA sequence determination has revealed the presence of UL9 gene homologues (genes 51 and 53, respectively;Davison & Scott, 1986;McGeoch et al, 1988;Telford et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein

Arbuckle¹,

Stow²

1993

Journal of General Virology

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The herpes simplex virus type 1 origin-binding protein is encoded by gene UL9. We previously described a plasmid, pB 1, which encodes a fusion protein containing only the C-terminal 317 amino acids of the UL9 polypeptide and showed that this product retains sequence-specific DNA-binding ability. Two series of pB1 mutants have now been constructed and the polypeptides were tested for origin-binding activity. Using C-terminal truncations, we show that the Cterminal 34 amino acids of UL9 are dispensable for binding and that essential residues lie between positions 801 and 818. Analysis of a series of mutants containing insertions of four amino acids at various positions identified regions of the DNA-binding domain in which alterations either abolished or had relatively little effect upon binding activity. Two mutants which were intermediate in their binding activities also exhibited temperature-or sequence-specific effects.

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Section: Discussionmentioning

confidence: 99%

A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein

Arbuckle¹,

Stow²

1993

Journal of General Virology

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“…HHV-1 and HHV-2 are 16 to 33 % variant in sequences examined across their genomes (McGeoch et al, 1987). The HHV-2 gH sequence has not been determined, but the gH sequences from EHV-1 and EHV-4 are available for comparison; the amino acid sequences are 15% variant and of different length, 848 and 855 residues, respectively (Nicolson et al, 1990). Again this is distinct from variation between strains of HCMV and HHV-6.…”

Section: Relationships Between Hhv-6 Strain Variants and With Other Hmentioning

confidence: 99%

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Gompels

Carrigan

Carss

et al. 1993

Journal of General Virology

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Fifteen human herpesvirus 6 (HHV-6) strain variants were analysed by PCR amplifications, restriction enzyme site polymorphism and sequence analyses. Three DNA regions were chosen for study: a fragment of a variable glycoprotein gene (210 bp), the conserved glycoprotein H (gH) gene complete with intergenic sequences (2381 bp) and the 5' intergenic region with the Nterminal coding sequence of gH up to a polymorphic BamHI site (427 bp). Infected cell DNA from five laboratory reference strains including GS, Ul102, AJ, Z29 and KF were examined together with DNA from peripheral blood lymphocytes infected with HHV-6 reactivated from blood and/or marrow from five bone marrow transplant (BMT) patients. Separate blood and marrow isolates were obtained from four BMT patients. In addition, HHV-6 sequences were examined directly from one of six Hodgkin's lymphomas and six B cell proliferations which contained HHV-6 DNA as detected by PCR amplification. The results show two groups of very closely related but heterogeneous strains which correlate with previous groupings by antigenic and restriction site differences. These are variant A strains (including laboratory strains GS, Ul102 and AJ) and variant B strains (including laboratory strains Z29 and KF, the Hodgkin's lymphoma strain, and the nine BMT patient isolates). Variations between the groups were 4 to 6 % in nucleotide sequence and 5 to 8.5 % in amino acid sequence. Within each group maximum heterogeneity was observed in different genes. Variant A strains differed by 2-0% in the variable glycoprotein gene sequence whereas variant B strains were identical in this region; conversely, variant B strains differed by 2 to 3 % in the gH N-terminal and intergenic sequences whereas variant A strains differed there by less than 0'2%. There was evidence for sequence drift independent of selection: relationships between the groups were shown by analyses of amino acid sequence, coding nucleotide sequence as well as intergenic sequence, and the B variant-specific BamHI site in the gH gene was due to a non-coding nucleotide substitution. There was little evidence for in vivo or in vitro variation: the gH nucleotide sequence from the uncultured lymphoma strain (first variant B gH gene identified) was almost identical to the gH sequence from four BMT isolates, and matched BMT isolates from blood and marrow were identical or with a single nucleotide substitution. The overall variation observed between the HHV-6 strain groups was similar or less than that seen between human cytomegalovirus strains such as AD169 or Towne, but deafly distinct from the much greater divergence between currently designated herpesvirus species.

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“…2. This compares to 11 such sites in the VZV and EHV-1 and -4 gHs, eight in herpesvirus saimiri (HVS), seven in HSV-1, six in HCMV, five in EBV and three in PRV (Keller et al, 1987;Robertson et al, 1991;Nicolson et al, 1990;Gompels et al, 1988;Gompels & Minson, 1986;McGeoch & Davison, 1986;Cranage et al, 1988;Baer et al, 1984;Klupp & Mettenleiter, 1991). Only one of these sites shows conservation of position in all eight gH sequences.…”

mentioning

confidence: 99%

“…Further primer extension sequencing of BamHI-F itself, to provide data for the 500 bp downstream of the MDV gH gene, revealed no direct repeat elements or putative replication origin sequences, as has been found in this position for equine herpesvirus (EHV)-I and -4 and pseudorabies virus (PRV) (Robertson et al, 1991;Nicolson et al, 1990;Klupp & Mettenleiter, 1991). The repeats in PRV are located 440 bp downstream of the gH stop codon and thus our results do not preclude the existence of an equivalent region further downstream in MDV.…”

mentioning

confidence: 99%

Identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek's disease virus and the herpesvirus of turkeys

Scott

Smith

Ross

et al. 1993

Journal of General Virology

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The Nucleotide Sequence of an Equine Herpesvirus 4 Gene Homologue of the Herpes Simplex Virus 1 Glycoprotein H Gene

Cited by 38 publications

References 39 publications

A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein

A mutational analysis of the DNA-binding domain of the herpes simplex virus type 1 UL9 protein

Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin's lymphoma and bone marrow transplant patients

Identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek's disease virus and the herpesvirus of turkeys

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