Transcript Analysis of The Equine Herpesvirus 1 Glycoprotein B Gene Homologue and Its Expression by a Recombinant Vaccinia Virus

Bell, Christopher W.; Boyle, D. B.; Whalley, J. M.

doi:10.1099/0022-1317-71-5-1119

Cited by 16 publications

(7 citation statements)

References 39 publications

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“…However, we cannot assure only from this result whether only one or both RNA transcripts are specific to the gB homologous gene. Similar result was reported in the case of EHV-1 [3] and suggested that overlapping of' ORFs of gB homologous and ICP 18.5 homologous genes resulted in two RNA transcripts, and that the shorter RNA transcript was specific to the gB homologue and the longer to the ICP 18.5 homologue. Nevertheless further analysis of the RNA transcripts specific to FHV-t gB homologue will be required.…”

supporting

confidence: 84%

Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein B

Maeda

Horimoto

Norimine

et al. 1992

Archives of Virology

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The nucleotide sequence of the glycoprotein B (gB) homologous gene of feline herpesvirus type 1 (FHV-1) was determined. The gene was found to be located within a 9.6 kbp SalI fragment by Southern-blot hybridization with a probe derived from the herpes simplex virus type 1 (HSV-1) gB DNA sequence. Furthermore, the predominant portion of the coding sequences was mapped to a 1.9 kbp Hin cII-EcoRI and its flanking 2.7 kbp Eco RI-Eco RI subfragments in the 9.6 kbp SalI fragment. The entire nucleotide sequence revealed that the FHV-1 gB homologous gene is capable of encoding a polypeptide of 948 amino acids. The predicted precursor polypeptide derived from this open reading frame could have a calculated M(r) of 106 kDa in unglycosylated form and contains ten potential N-linked glycosylation sites and a probable internal proteolytic cleavage site. By Northern-blot analysis using portions of the open reading frame as a probe, 3.9 and 3.3 kb RNA transcripts were identified in FHV-1 infected cells. The alignment of the amino acid sequence of the FHV-1 gB homologue with those of 14 other herpesviruses revealed that 10 cysteine residues were completely conserved. Meanwhile, when evolutionary trees were generated among these herpesvirus gB counterparts, the FHV-1 gB homologous nucleotide sequence seems to be closely related to equine herpesvirus type 4 and its amino acid sequence to pseudorabies virus.

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supporting

confidence: 84%

Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein B

Maeda

Horimoto

Norimine

et al. 1992

Archives of Virology

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“…Common features include replication in the respiratory mucosae, the development of pneumonitis, cell-associated viremia, and abortion (7,8). Various EHV-1 glycoproteins, including gB, gC, gD, and gH, were capable of inducing neutralizing antibodies (9)(10)(11)(12)(13)(14). EHV-1-specific CD8 ϩ class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) were identified in the peripheral blood mononuclear cells of infected horses, reaching maximal levels 2 to 3 weeks postinfection (15,16).…”

mentioning

confidence: 99%

Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge

Kim

Shakya

O’Callaghan

2016

J Virol

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Equine herpesvirus 1 (EHV-1) is a major pathogen affecting equines worldwide. The virus causes respiratory disease, abortion, and, in some cases, neurological disease. EHV-1 strain KyA is attenuated in the mouse and equine, whereas wild-type strain RacL11 induces severe inflammation of the lung, causing infected mice to succumb at 4 to 6 days postinfection. Our previous results showed that KyA Equine herpesvirus 1 (EHV-1), a member of the family Herpesviridae and the subfamily Alphaherpesvirinae, is the causative agent of a number of equine pathological states, including severe disease of the central nervous system, respiratory infections, and abortion storms (1-4). Respiratory transmission of this highly contagious virus has resulted in disastrous outbreaks of disease in domestic horse populations and has had significant economic impact on the equine industry. EHV-1 infection of the horse generates a short-lived humoral response but does not confer long-term protection, since disease often occurs following natural infection (5, 6).A model that closely mimics EHV-1 infection in the natural host has been established with various strains of mice (7). Common features include replication in the respiratory mucosae, the development of pneumonitis, cell-associated viremia, and abortion (7,8). Various EHV-1 glycoproteins, including gB, gC, gD, and gH, were capable of inducing neutralizing antibodies (9-14). EHV-1-specific CD8 ϩ class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) were identified in the peripheral blood mononuclear cells of infected horses, reaching maximal levels 2 to 3 weeks postinfection (15,16). Intranasal inoculation of mice with an attenuated EHV-1 strain, KyA, resulted in the production of a primary virus-specific CTL response in the draining mediastinal lymph nodes (17). In this model, EHV-1-specific CTL activity was mediated by class I MHC-restricted CD8 ϩ T cells (17). The nonpathogenic EHV-1 strain Kentucky A (KyA) is attenuated in mice and horses, whereas the wild-type pathogenic strain RacL11 induces severe inflammatory cell infiltration in the lung, such that infected mice succumb at 4 to 6 days postinfection (dpi) (Fig. 1) (18-23). RacL11 was isolated from an aborted fetus (24). KyA has a long history of passage outside the natural host and

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“…The mature form of this protein exists as an 895-amino-acid glycosylated heterodimer located in the viral envelope (20,30,53). It is known to be a target of the equine humoral immune system and can stimulate the production of monoclonal antibodies which neutralize virus in vitro and mediate passive protection in vivo (5,39,41,46). Vaccinia virus recombinants expressing EHV-1 gB elicit the production of neutralizing antibody in guinea pigs and, when inoculated into hamsters, can protect the animals against subsequent lethal challenge (21).…”

Section: Discussionmentioning

confidence: 99%

Detection of antibodies against equine herpesvirus types 1 and 4 by using recombinant protein derived from an immunodominant region of glycoprotein B

et al. 1993

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The N-terminal fragment comprising residues +1 to +50 (gBl-50) of equine herpesvirus type 1 (EHV-1) glycoprotein B was expressed as a glutathione S-transferase fusion protein in Escherichia coli. Recombinant gBl-50 (rgBl-50) was recognized in immunoblots by sera from rabbits immunized with EHV-1 and by convalescent-phase sera from horses with natural EHV-1 infections. An enzyme-linked immunosorbent assay (ELISA) for monitoring antibody levels against EHV-1 was developed by using rgBl-50, and its specificity was assessed with a panel of reference antisera against other equine viruses. A specific cross-reaction was detected with EHV-4, which was confirmed by inhibition ELISA. Convalescent-phase sera from horses with natural EHV-1 or EHV-4 infections possessed antibody titers against rgBl-50 ranging from 1:2,000 to 1:64,000, indicating the presence of an immunodominant antigenic site. The study demonstrated the potential application of rgBl-50 as a diagnostic antigen and highlights the glutathione S-transferase fusion system as a simple and effective method of producing purified milligram quantities of antigen.

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Transcript Analysis of The Equine Herpesvirus 1 Glycoprotein B Gene Homologue and Its Expression by a Recombinant Vaccinia Virus

Cited by 16 publications

References 39 publications

Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein B

Identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein B

Immunization with Attenuated Equine Herpesvirus 1 Strain KyA Induces Innate Immune Responses That Protect Mice from Lethal Challenge

Detection of antibodies against equine herpesvirus types 1 and 4 by using recombinant protein derived from an immunodominant region of glycoprotein B

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