2001
DOI: 10.1590/s1415-47572001000100033
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Abstract: Nucleotide synthesis is of central importance to all cells. In most organisms, the purine nucleotides are synthesized de novo from non-nucleotide precursors such as amino acids, ammonia and carbon dioxide. An understanding of the enzymes involved in sugarcane purine synthesis opens the possibility of using these enzymes as targets for chemicals which may be effective in combating phytopathogen. Such an approach has already been applied to several parasites and types of cancer. The strategy described in this pa… Show more

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Cited by 4 publications
(4 citation statements)
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References 14 publications
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“…Nucleotide Sequence Analysis Previous data-mining work (Jancso et al 2001) identified seven full-length and nine partial prs clones distributed in 16 tissue-specific and one normalized sugarcane cDNA library. One of these clones (SCACRZ3035C11) from the plat root library was selected and a 1,194-bp DNA fragment was PCR amplified.…”
Section: Resultsmentioning
confidence: 99%
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“…Nucleotide Sequence Analysis Previous data-mining work (Jancso et al 2001) identified seven full-length and nine partial prs clones distributed in 16 tissue-specific and one normalized sugarcane cDNA library. One of these clones (SCACRZ3035C11) from the plat root library was selected and a 1,194-bp DNA fragment was PCR amplified.…”
Section: Resultsmentioning
confidence: 99%
“…The SUCEST (http://sucest.lad.dcc.unicamp.br/en/) databank identified 16 PRS coding EST clones, seven full length and nine partial sequences, distributed in 6 of the 16 tissues that had libraries generated (Jancso et al 2001). The analysis done by Jancso et al (2001) was unable to identify a total number of PRS isoforms present in the sugarcane genome, but it is clear that a number of isoforms are present.…”
Section: Sugarcane Prs Sequence Characterization Cloning and Expresmentioning
confidence: 99%
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“…The SUCEST strategy was based on the random sequencing of EST (expressed sequence tag) fragments obtained from different plant tissues and allows investigation of the expressed genes of a complex genome. Based on the knowledge generated from the SUCEST project, several important genes have been identi®ed, including representatives of the purine-salvage and de novo synthesis pathways (Jancso et al, 2001). In the present report, we describe the puri®cation, crystallization and preliminary crystallographic analysis of the sugar cane PRPP synthase enzyme as part of a continuing study of de novo and salvage pathway enzymes in several organisms.…”
Section: Introductionmentioning
confidence: 99%