2004
DOI: 10.1107/s1744309104025825
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Abstract: Phosphoribosylpyrophosphate synthases (PRS; EC 2.7.6.1) are enzymes that are of central importance in several metabolic pathways in all cells. The sugar cane PRS enzyme contains 328 amino acids with a molecular weight of 36.6 kDa and represents the ®rst plant PRS to be crystallized, as well as the ®rst phosphateindependent PRS to be studied in molecular detail. Sugar cane PRS was overexpressed in Escherichia coli, puri®ed and crystallized using the hangingdrop vapour-diffusion method. Using X-ray diffraction e… Show more

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