2008
DOI: 10.1007/s11105-008-0043-6
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Abstract: Phosphoribosyl pyrophosphate synthetase (PRS-EC:2.7.6.1) is an important enzyme present in several metabolic pathways, thus forming a complex family of isoenzymes. However, plant PRS enzymes have not been extensively investigated. In this study, a sugarcane prs gene has been characterized from the Sugar Cane Expressed Sequence Tag Genome Project. This gene contains a 984-bp open reading frame encoding a 328-amino acid protein. The predicted amino acid sequence has 77% and 78% amino acid sequence identity to Ar… Show more

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Cited by 7 publications
(5 citation statements)
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“…Some controversy exists on this point, as characterization of sugarcane class II PRPP synthase revealed a molecular mass of 214 kDa, i.e., a value consistent with a hexameric quaternary structure. Like S. oleracea PRPP synthase isozymes 3 and 4, the activity of sugarcane PRPP synthase is independent of P i (129).…”
Section: Class II Prpp Synthasesmentioning
confidence: 99%
“…Some controversy exists on this point, as characterization of sugarcane class II PRPP synthase revealed a molecular mass of 214 kDa, i.e., a value consistent with a hexameric quaternary structure. Like S. oleracea PRPP synthase isozymes 3 and 4, the activity of sugarcane PRPP synthase is independent of P i (129).…”
Section: Class II Prpp Synthasesmentioning
confidence: 99%
“…Most of the basic molecular and genetic analyses remain inconclusive in sugarcane due to the lack of genomic information of this complex genome. Typically, expressed sequence tags (EST) resources are used for sugarcane gene and gene family discovery, and these were the sole resource prior to the release of sorghum genome [ 7 , 8 ]. Sorghum is the closest diploid species of Saccharum, and has a genome size about 760 Mb which has been sequenced [ 9 ].…”
Section: Introductionmentioning
confidence: 99%
“…For the most part, expressed sequence tags (EST) resources have been the sole resource for sugarcane gene and gene family discovery [7,8]. The recent sequencing and annotation of sorghum bicolor’s genome, the closest diploid relative of sugarcane in the Andropogonae tribe, has served as an indispensable resource for sugarcane genomic studies [9].…”
Section: Introductionmentioning
confidence: 99%