Identification of spring viraemia of carp virus (SVCV) by combined RT-PCR and nested PCR

Characterization of two thymosins as immune-related genes in common carp (Cyprinus carpio L.)

“…Fishes in the control group were injected with the same amount of saline. At 5 days postinfection, RT-PCR and nested PCR reactions (Koutna et al, 2003) in spleen and head kidney tissues confirmed that the experimental carp were infected by SVCV (figure not shown). The SVCV-infected carp at 0 d, 1 d, 3 d, 5 d, 7 d, 10 d after infection (3 infected carp as a group, n = 3) and the various tissue samples of control (n = 3) were immediately removed and frozen in liquid nitrogen, and/or stored at −80°C for RNA isolation.…”

Cloning of common carp SOCS-3 gene and its expression during embryogenesis, GH-transgene and viral infection

“…In the SVCVinfection group, each fish was intraperitoneally injected with 250e300 mL SVCV at a dosage of approximately 10 7 TCID 50 /kg body weight, while fish in the control group were injected with the same amount of saline. At 5 days post-infection, RT-PCR and nested PCR reactions [23] in spleen and head kidney tissues confirmed that the experimental carp were infected by SVCV (Figure not Total RNAs were all extracted from sperm, eggs, the embryos and larvae at different developmental stages of carp, and the various tissues (gill, spleen, thymus, head kidney, kidney, liver, muscle, skin, brain, peripheral blood and intestine) of carp (the GHtransgenic carp, the infected carp at different time points and control carp) using Trizol Reagent (Invitrogen) following the user's manual. The quality and concentration of total RNA were analyzed by agarose gel electrophoresis and optical density reading at 260 and 280 nm.…”

Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

“…In the SVCV-infection group, each fish was intraperitoneally injected with 150-200 mL SVCV at a dosage of approximately 10 6 TCID 50 kg À1 body weight, while fish in the control group were injected with the same amount of saline. RT-PCR and nested PCR reactions were used to confirm that the experimental carp were infected by SVCV [28]. At 5 days post-infection, the presence of viral RNA was detected in RNA samples from liver and kidney tissues (not shown), the control and SVCV-infected carp were sacrificed and the tissue samples were frozen immediately in liquid nitrogen and stored at À80 C until RNA isolation.…”