Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

Huang, Rong; Gao, Longying; Wang, Yaping; Hu, Wei; Guo, Qinghai

doi:10.1016/j.fsi.2008.10.013

Cited by 33 publications

(19 citation statements)

References 41 publications

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“…NKEF was up‐regulated in all infected samples as compared to control. It was earlier observed that this gene was up‐regulated in response to LPS, viral and parasitic infections (Bethke et al., ; Chen, Wu, Niu, Shi & Li, ; Chen et al., ; Cuesta & Tafalla, ; Dong, Xiang & Shao, ; Huang, Gao, Wang, Hu & Guo, ; Loo, Sutton & Schuller, ; Ordás, Cuesta, Mercado, Bols & Tafalla, ; Pérez‐Sánchez, Bermejo‐Nogales, Calduch‐Giner, Kaushik & Sitjà‐Bobadilla, ; Utke et al., ). The transcript level was high in infected samples because it has role in inflammatory response, tissue repair by protecting cells from oxidative stress after infection and protection against cell death (Bethke et al., ; Ishii, Warabi & Yanagawa, ).…”

Section: Discussionmentioning

confidence: 96%

Labeo rohitaandArgulus siamensisinfection: Host size, local inflammatory reaction and immunity modulate ectoparasite load on fish

et al. 2017

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Parasite species often show a heterogeneous, highly dispersed pattern of infestation within hosts. Varieties of factors including morphological, physiological, immunological and nutritional characteristics affect the infestation level of a specific parasite in homogenous pray. Limited attempts, however, have been made to explore such underlying drivers of infestation pattern. Here, three stages of Labeo rohita (fingerling, juvenile and pre-adult) were challenged with ectoparasite, Argulus siamensis in same aquaria. The parasite load on individuals was determined at 5-day interval for 1 month. The load was found to be highest in pre-adult stage followed by juveniles and fingerlings. On day 20 post infection, the load of parasite on pre-adult fish was high along with detectable skin damages. Skin tissues were collected for immune gene expression analysis and histopathology. Histological studies showed increased melanization in the dermis and mild inflammatory cellular reactions in pre-adult fish whereas, massive subcutaneous myositis with engorged blood vessels were observed in fingerlings. The expression levels of various inflammation and innate immune-related genes viz., interleukin (IL)-8, IL-10, IL-11, IL-15, natural killer enhancing factor, toll-like receptor 4, apolipoprotein A-I and immunoglobulin Z were significantly high in skin samples of infected fingerlings as compared to other two growth stages or controls of each stage. On the other hand, the expression of immunoglobulin M was down-regulated in all infected samples as compared to their respective controls. The results thus depict that local immuno-inflammatory response plays a significant role in determining susceptibility of host in intra-specific group, and has important implications for ecology and aquaculture.

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Section: Discussionmentioning

confidence: 96%

Labeo rohitaandArgulus siamensisinfection: Host size, local inflammatory reaction and immunity modulate ectoparasite load on fish

et al. 2017

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“…In fish, although there is only few data available concerning the granule-dependent pathways with PRF and GRZ, the involvement of these two proteins in cytotoxic activities has been established [43,44]. Furthermore, at the gene level, PRF1 but not PRF2 nor PRF3 were up-regulated in crucian carp CTLs from allo-sensitized fish [17] whilst the GRZ gene was up-regulated in common carp after viral infection [28]. Further studies are still needed to ascertain the exact role of these proteins in the cytotoxic activities of rainbow trout monocyte/macrophages.…”

Section: Discussionmentioning

confidence: 99%

Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity and the perforin/granzyme pathway in the rainbow trout RTS11 cell line

Ordás¹,

Cuesta²,

Mercado

et al. 2011

Fish & Shellfish Immunology

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“…Noteworthily, BR-C Z4, FOXD3, Elk-1 and HNF-1 have been found in human GzmK promoter, and BR-C Z4, HLF and CDP CR1 have been found in human GzmA promoter. While, CDP CR1, BR-C Z4, Pax-6, Elk-1, FOXD3 and HNF-1 have also been reported in carp NKEF-B promoter [48]. This seems that carp NKEF-B and GzmA/K gene may be regulated by the same system and cooperate to play their role in carp immune system.…”

Section: Discussionmentioning

confidence: 91%

Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease

Huang

Zhong

Liu

et al. 2010

Fish & Shellfish Immunology

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Structure, organization and expression of common carp (Cyprinus carpio L.) NKEF-B gene

Cited by 33 publications

References 41 publications

Labeo rohitaandArgulus siamensisinfection: Host size, local inflammatory reaction and immunity modulate ectoparasite load on fish

Labeo rohitaandArgulus siamensisinfection: Host size, local inflammatory reaction and immunity modulate ectoparasite load on fish

Viral hemorrhagic septicaemia virus (VHSV) up-regulates the cytotoxic activity and the perforin/granzyme pathway in the rainbow trout RTS11 cell line

Identification and characterization of common carp (Cyprinus carpio L.) granzyme A/K, a cytotoxic cell granule-associated serine protease

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