Identification of rose phenylacetaldehyde synthase by functional complementation in yeast

Farhi, Moran; Lavie, Orly; Masci, Tania; Hendel-Rahmanim, Keren; Weiss, David; Abeliovich, Hagai; Vainstein, Alexander

doi:10.1007/s11103-009-9564-0

Cited by 52 publications

(48 citation statements)

References 50 publications

(101 reference statements)

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“…The diurnal accumulation pattern of transcripts coding for scent-related biosynthetic genes is complex, while the emission of most scent compounds is synchronized (Kolosova et al, 2001;Schuurink et al, 2006;Hendel-Rahmanim et al, 2007;Farhi et al, 2009). It has been suggested that circadian and light regulation of scent production was adapted by plants, from an evolutionary standpoint, relatively recently (Hendel-Rahmanim et al, 2007) and that a combination of various mechanisms was harnessed by plants for rhythmic production/emission of scent compounds (Kolosova et al, 2001;Simkin et al, 2004;HendelRahmanim et al, 2007).…”

Section: Quantitative Real-time Pcr Analysis Of Cs (A) CM (B) Pal2 mentioning

confidence: 99%

EOBII, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia

et al. 2010

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Floral scent, which is determined by a complex mixture of low molecular weight volatile molecules, plays a major role in the plant's life cycle. Phenylpropanoid volatiles are the main determinants of floral scent in petunia (Petunia hybrida). A screen using virus-induced gene silencing for regulators of scent production in petunia flowers yielded a novel R2R3-MYB–like regulatory factor of phenylpropanoid volatile biosynthesis, EMISSION OF BENZENOIDS II (EOBII). This factor was localized to the nucleus and its expression was found to be flower specific and temporally and spatially associated with scent production/emission. Suppression of EOBII expression led to significant reduction in the levels of volatiles accumulating in and emitted by flowers, such as benzaldehyde, phenylethyl alcohol, benzylbenzoate, and isoeugenol. Up/downregulation of EOBII affected transcript levels of several biosynthetic floral scent-related genes encoding enzymes from the phenylpropanoid pathway that are directly involved in the production of these volatiles and enzymes from the shikimate pathway that determine substrate availability. Due to its coordinated wide-ranging effect on the production of floral volatiles, and its lack of effect on anthocyanin production, a central regulatory role is proposed for EOBII in the biosynthesis of phenylpropanoid volatiles.

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Section: Quantitative Real-time Pcr Analysis Of Cs (A) CM (B) Pal2 mentioning

confidence: 99%

EOBII, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia

et al. 2010

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“…Volatile phenylpropanoids include, among others, eugenol, isoeugenol, and vanillin, as well as C6-C1 benzenoids, such as benzaldehyde, benzyl alcohol, and methylbenzoate (Boatright et al, 2004;Long et al, 2009;Van Moerkercke et al, 2009). Another group of volatile phenylpropanoids derive from Phe via the action of phenylacetaldehyde synthase, resulting in phenylacetaldehyde and downstream volatiles (Kaminaga et al, 2006;Tieman et al, 2006;Farhi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The R2R3-MYB–Like Regulatory Factor EOBI, Acting Downstream of EOBII, Regulates Scent Production by Activating ODO1 and Structural Scent-Related Genes in Petunia

et al. 2012

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Flower scent is a highly dynamic trait, under developmental, spatial, and diurnal regulation. The mechanism governing scent production is only beginning to be unraveled. In petunia (Petunia hybrida), EMISSION OF BENZENOIDS II (EOBII) controls transcription of both the shikimate pathway-regulating MYB factor ODORANT1 (ODO1) and phenylpropanoid scent-related structural genes. A promoter-activation screen identified an R2R3-MYB-like regulatory factor of phenylpropanoid volatile biosynthesis acting downstream of EOBII, designated EOBI. EOBI silencing led to downregulation of ODO1 and numerous structural scent-related genes from both the shikimate and phenylpropanoid pathways. The ability of EOBI to directly activate ODO1, as revealed by electrophoretic mobility shift assay and yeast one-hybrid analysis, place EOBI upstream of ODO1 in regulating substrate availability for volatile biosynthesis. Interestingly, ODO1-silenced transgenic petunia flowers accumulated higher EOBI transcript levels than controls, suggesting a complex feedback loop between these regulatory factors. The accumulation pattern of EOBI transcript relative to EOBII and ODO1, and the effect of up/downregulation of EOBII on transcript levels of EOBI and ODO1, further support these factors' hierarchical relationships. The dependence of scent production on EOBI expression and its direct interaction with both regulatory and structural genes provide evidence for EOBI's wide-ranging involvement in the production of floral volatiles.

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“…In the world of orchids, more than 400 orchids, either species or hybrid, have been reported to emit fragrance during the day or in the evening (Kaiser 1993;Frowine 2005). Unfortunately, scientific studies on the orchids' fragrance are still not as well established as in other scented flower including petunia, rose and snapdragon (Dudareva et al 2003;Verdonk et al 2003;Boatright et al 2004;Nagegowda et al 2008;Farhi et al 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Identification of volatile compounds has been carried out on the scent of more than 180 orchid species and hybrids including Cattleya araguaiensis, Dendrobium carniferum, Oncidium curcutum, Vanda tessellata (Kaiser 1993) and Phalaenopsis bellina (Hsiao et al 2006) by GC-MS. Based on the GC-MS results, monoterpenoids and sesquiterpenoids were found to be highly distributed among the orchid species. Identification of floral scent compounds in orchids has led to extensive biochemical and molecular studies on other scented plants such as Clarkia breweri, Petunia hybrida, Antirrhinum majus and Rosa hybrida (Dudareva et al 2003;Kaminaga et al 2006;Nagegowda et al 2008;Farhi et al 2010). However, the floral scent biosynthesis pathways of orchids are yet far from being understood (Hsiao et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

et al. 2011

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The aims of this study were to isolate and characterize putative fragrance-related cDNAs from the floral cDNA library of Vanda Mimi Palmer, an orchid hybrid that has won several international awards for its sweet fragrance. A total of 1,000,000 pfu were screened by hybridizing cDNA library plaques with fully open flower cDNA probe of Vanda Mimi Palmer representing all mRNAs expressed during daytime. The clones that gave positive signals were in vivo excised and PCR-amplified inserts were subjected to reverse-Northern analysis by hybridizing with cDNA probes of fully open flower of Vanda Mimi Palmer (fragrant orchid) and its bud or fully open flower of Vanda Tan Chay Yan (non-fragrant orchid) separately. The clones up-regulated in fully open flower stage of Vanda Mimi Palmer compared to its bud stage or fully open flower of Vanda Tan Chay Yan were sequenced. Sequence analyses showed the presence of eight putative fragrance-related cDNAs of which two were putative Vanda Mimi Palmer 4-(cytidine 5 0 -diphospho)-2-Cmethyl-D-erythritol kinase (VMPCMEK) and Vanda MimiPalmer cytochrome P450 protein (VMPCyP450). These two transcripts were selected for full-length cDNA isolation and expression analysis by real-time RT-PCR. The VMPCMEK transcript encodes a polypeptide of 400 amino acid residues, while the VMPCyP450 encodes 538 amino acid residues. Relative expression analysis of VMPCMEK and VMPCyP450 transcripts by real-time RT-PCR showed up-regulated expressions in floral tissues compared to vegetative tissues, and both were found to be developmentally regulated.

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Identification of rose phenylacetaldehyde synthase by functional complementation in yeast

Cited by 52 publications

References 50 publications

EOBII, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia

EOBII, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia

The R2R3-MYB–Like Regulatory Factor EOBI, Acting Downstream of EOBII, Regulates Scent Production by Activating ODO1 and Structural Scent-Related Genes in Petunia

Screening, isolation, and molecular characterization of putative fragrance-related transcripts from Vanda Mimi Palmer

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