<i>EOBII</i>, a Gene Encoding a Flower-Specific Regulator of Phenylpropanoid Volatiles' Biosynthesis in Petunia

Spitzer-Rimon, Ben; Marhevka, Elena; Barkai, Oren; Marton, Ira; Edelbaum, Orit; Masci, Tania; Prathapani, Naveen-Kumar; Shklarman, Elena; Ovadis, Marianna; Vainstein, Alexander

doi:10.1105/tpc.109.067280

Cited by 145 publications

(225 citation statements)

References 60 publications

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“…Phylogenetically, FaEOBII shares high amino acid homology with members of this subgroup of MYB TFs from other plants, such as PsMYB26 (Pisum sativum), NlxNsMYB305 (Nicotiana langsdorffii 3 Nicotiana sanderae), AmMYB305 (Antirrhinum major), AmMYB340 (A. major), AtMYB24 (Arabidopsis), AtMYB21 (Arabidopsis), and PhEOBII (petunia). Functional characterization of all of these TFs has demonstrated that they are involved in the regulation of the volatile benzenoid phenylpropanoid metabolic pathway (Sablowski et al, 1994;Moyano et al, 1996;Uimari and Strommer, 1997;Shin et al, 2002;Li et al, 2006;Liu et al, 2009;Spitzer-Rimon et al, 2010). All of these bioinformatic analyses suggested that FaEOBII could be an R2R3 MYB TF potentially involved in the regulation of those metabolic pathways related to volatile phenylpropanoid production in ripe receptacles.…”

Section: Sequence Analysis Of the Faeobii Gene And Proteinmentioning

confidence: 99%

“…This suggests that these genes could be regulated by the synergistic action of EOBII and other TFs. On the other hand, PAL, CFAT, and IGS transcripts were up-regulated, indicating a direct positive regulatory role of the expression of these genes by EOBII (Spitzer-Rimon et al, 2010). Besides, ODO1 was demonstrated to activate the promoter of 5-ENOL-PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS), hence regulating the floral shikimate pathway that produces benzenoid/ pheylpropanoid volatiles (Verdonk et al, 2005).…”

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confidence: 94%

“…The orthologous gene of FaEOBII in petunia (PhEOBII) has been shown to be involved in eugenol production (Spitzer-Rimon et al, 2010). Thus, we analyzed eugenol production in strawberry fruit receptacles during ripening (Fig.…”

Section: The Expression Of Faeobii Is Highly Expressed In Ripe Fruitmentioning

confidence: 99%

“…PhEOBII binds and activates the promoter of ODO1 through the union to a MYB-binding site (MBS), indicating a hierarchical link between both TFs (Van Moerkercke et al, 2011). Previous work carried out on protoplast extracted from Arabidopsis (Arabidopsis thaliana) leaves showed binding of PhEOBII to the petunia ISOEUGENOL SYNTHASE (IGS) and to the tobacco (Nicotiana tabacum) Phe ammonia lyase B promoters (Spitzer-Rimon et al, 2010). Reduction of EOBII transcript levels in petunia petals by virus-induced gene silencing leads to a down-regulation of the expression of genes involved in the shikimate and phenylpropanoid pathways, such as CHORISMATE SYNTHASE (CS), CHORISMATE MUTASE (CM), PHENYLALANINE AMMONIA LYASE (PAL2), CONIFERYL ALCOHOL ACETYLTRANSFERASE (CFAT), IGS, BENZOYL-COENZYME A:BENZYL ALCOHOL/ PHENYLETHANOL BENZOYLTRANSFERASE (BPBT), and ODO1, whereas no changes were detected in the expression levels of CHALCONE ISOMERASE (CHI) or FLAVANONE 3-HYDROXYLASE (FHT).…”

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confidence: 99%

“…In petunia (Petunia hybrida), the function of EMISSION OF BENZE-NOID II (PhEOBII) and ODORANT1 (ODO1), two R2R3-MYB TFs, have been described as regulators of the volatile and nonvolatile phenylpropanoid biosynthesis pathway (Verdonk et al, 2005;Spitzer-Rimon et al, 2010). PhEOBII binds and activates the promoter of ODO1 through the union to a MYB-binding site (MBS), indicating a hierarchical link between both TFs (Van Moerkercke et al, 2011).…”

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confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria 3 ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and functionally characterized an R2R3 MYB transcription factor (EMISSION OF BENZENOID II [FaEOBII]) that seems to be the orthologous gene of PhEOBII from Petunia hybrida, which contributes to the regulation of eugenol biosynthesis in petals. The expression of FaEOBII was ripening related and fruit receptacle specific, although high expression values were also found in petals. This expression pattern of FaEOBII correlated with eugenol content in both fruit receptacle and petals. The expression of FaEOBII was repressed by auxins and activated by abscisic acid, in parallel to the ripening process. In ripe strawberry receptacles, where the expression of FaEOBII was silenced, the expression of CINNAMYL ALCOHOL DEHYDROGENASE1 and FaEGS2, two structural genes involved in eugenol production, was down-regulated. A subsequent decrease in eugenol content in ripe receptacles was also observed, confirming the involvement of FaEOBII in eugenol metabolism. Additionally, the expression of FaEOBII was under the control of FaMYB10, another R2R3 MYB transcription factor that regulates the early and late biosynthetic genes from the flavonoid/phenylpropanoid pathway. In parallel, the amount of eugenol in FaMYB10-silenced receptacles was also diminished. Taken together, these data indicate that FaEOBII plays a regulating role in the volatile phenylpropanoid pathway gene expression that gives rise to eugenol production in ripe strawberry receptacles.The octoploid cultivated strawberry (Fragaria 3 ananassa) is one of the most economically important, nonclimacteric, soft fruits, in which volatile compounds influence fruit flavor and aroma. Both characteristics contribute to the fruit organoleptic traits and are crucial factors to determine fruit quality.At present, extensive surveys on the components that contribute to strawberry flavor have been performed. In these studies, more than 360 volatiles have been identified (Latrasse, 1991;Nijssen, 1996;Zabetakis and Holden, 1997), but only 15 to 20 of them in wild varieties of strawberry are believed to be essential for sensory quality, together with nonvolatile sugars and organic acids (Schieberle and Hofmann, 1997). In contrast, in cultivated varieties of strawberry, only about six odor-active compounds have been identified as contributors to fruit flavor (Raab et al., 2006;Ulrich et al., 2007). Strawberry aroma is the result of the combined perception of fruity (ethyl butanoate, ethyl hexanoate, and methyl 2-methylbutanoat), green (Z-3-hexenal), sweaty (butanoic acid and 2-methylbutanoic acid), peach-like (g-decalactone), and caramel-like [4-hydroxy-2,5-dimethyl-3(2H)-furanone and 2,5-dimethyl-4-methoxy-3(2H)-furanone] flavor notes (Pyysalo et al., 1979;Larsen and Poll, 1992). Several wild strawb...

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Section: Sequence Analysis Of the Faeobii Gene And Proteinmentioning

confidence: 99%

mentioning

confidence: 94%

Section: The Expression Of Faeobii Is Highly Expressed In Ripe Fruitmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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Shikimate Pathway and Aromatic Amino Acid Biosynthesis

Tzin

Galili

Aharoni

2012

Encyclopedia of Life Sciences

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The shikimate pathway consists of seven enzymatic reactions whose end product chorismate is the precursor for the synthesis of the aromatic amino acids Phe, Tyr and Trp. In fungi and plants, chorismate is a precursor for many specialised metabolites (i.e. secondary metabolites) that play an important role in the plant's interaction with its environment. The shikimate pathway and aromatic amino acid biosynthesis have been extensively studied in a variety of microorganisms, fungi and plants. Furthermore, the dual involvement of the shikimate and aromatic amino acid biosynthesis pathways in central and specialised metabolism still raises major questions regarding the genes and enzymes involved, and their control, their evolutionary origins and coordinated regulation with genes of associated pathways in response to altered environmental conditions and diverse developmental programs. Key Concepts: The shikimate pathway is the only known pathway for biosynthesis of chorismate and the aromatic amino acids Phe, Tyr and Trp. The shikimate pathway is a bridge between central metabolism and specialised metabolism. The shikimate pathway occurs in various groups of microorganisms, plants and parasites, whereas it does not occur in animals. The pathway enzymes are being targeted for antimicrobial drug and herbicide design. Shikimic acid is an essential metabolite that may balance the metabolic status of the pathway. Chorismate is a branch point metabolite for aromatic amino acids and phenolic compounds. This is an ancient eukaryotic pathway which has been subject to diverse evolutionary processes. Several enzymes from these pathways are allosterically regulated by their end products: Phe, Tyr or Trp. The shikimate pathway and aromatic amino acids, and the specialised metabolites derived from them, simultaneously respond to rhythmic changes.

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