Identification of Quinolone and Colistin Resistance Genes in Escherichia Coli Strains Isolated from Mucosal Samples of Patients with Colorectal Cancer and Healthy Subjects

Abstract

Introduction: Antibiotic resistance and extensive use of antibiotics is one of the major causes of failure in antibiotic treatment. The purpose of this study was to investigate antibiotic resistance patterns and to identify resistance genes of quinolones and colistin in Escherichia coli. There are very few patents on E. coli isolated from colorectal cancer. So, this study demonstrated that some bacteria resistant to ciprofloxacin are not present resistance genes. As well as, new patterns for E. coli are presented for samples of patients with colorectal cancer. Materials and Methods: Of the three healthy, inflammatory bowel diseases (IBD) and colorectal cancer groups, 40 E. coli strains isolated after confirmation by biochemical and molecular methods. The susceptibility of isolates to antibiotics was investigated using disk diffusion test. After deoxyribonucleic acid (DNA) extraction, polymerase chain reaction (PCR) was used to identify genes encoding resistance to ciprofloxacin (qnr A, qnr B) and colistin (mcr-1). Results: The results showed that E. coli isolates from colorectal cancer patients had the highest resistance to piperacillin (67.5%), ceftazidime (47.5%), and cefepime (42.5%). Also, E. coli strains isolated from IBD patients showed resistance to antibiotic ceftazidime 13%. More than 95% of E. coli strains isolated from healthy people were susceptible to antibiotics. Based on the results, 18 (15%) E. coli strains were showed resistance to ciprofloxacin. The qnr A gene was detected in 61.11% isolate; however qnr B was detected in 9 (50%). Isolates resistant to colistin were not observed. Conclusion: These findings indicate increased resistance of E. coli to ciprofloxacin in comparison with prior studies. Further research in this field will increase our knowledge and more effective exposure to the antibiotic resistance of the pathogenic microorganisms.

Diffusely adherent and cyclomodulin- positive Escherichia coli are associated with colon diseases

“…Totally, prevalence of antibiotic resistance, in both studied groups (patients and healthy subjects), was higher than some other studies (23,27), However, there were some similarities; frequency of resistance against cipro oxacin in CRC group (35%) was approximately similar to reports of Mahmoudi et al (35%) (27) and Aibino et al (21.4%) (28), and the highest susceptibility was observed against amikacin and imipenem same as a previous report (27).…”

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

“…Hence, AIEC evolved into a disease-causing adaptive strain with mutant Fim H, which improved the ability of AIEC to colonize the intestine and induce inflammation in genetically susceptible hosts. A SNP analysis of FIM H can be performed to detect virulence of E. coli in IBD patients and can also be used in diagnostic or epidemiological investigations[ 107 ]. Interestingly, the protease methyldopa degraded the type I pili of AIEC and prevented AIEC from binding to mannosylated host receptors.…”