Role of adherent invasive <i>Escherichia coli</i> in pathogenesis of inflammatory bowel disease

Duan, Sheng-Lei; Dai, Yan-Cheng; Wu, Shi-Cheng

doi:10.12998/wjcc.v10.i32.11671

Cited by 13 publications

(16 citation statements)

References 138 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enterobacteriaceae has been reported to increase progressively with aging [68]. Enterobacteriaceae can induce in ammation in the host by releasing endotoxins which can contribute to muscle wasting and metabolic dysfunction [69,70]. The association found between obesity and Enterobacteriaceae in previous studies [71] further indicated the impact of combined obesity in myasthenia gravis on the gut bacterial community, which is consistent with our study.…”

Section: Discussionsupporting

confidence: 91%

Distinct Gut Microbiota Signatures in Order People with Sarcopenia without Obesity and Sarcopenic Obesity

Wang,

He,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Previous evidence suggests that gut dysbiosis plays an important role in the development and progression of sarcopenia and sarcopenic obesity, but evidence supporting this association is lacking. Thus, this study aimed to investigate the characteristics of gut microbiota in older people with sarcopenia and sarcopenic obesity. Methods The study involved 60 elderly individuals aged 65 years or older, recruited from Chongming, Shanghai, China. Participants were categorized into three groups based on the Asian Working Group for Sarcopenia 2019 (AWGS 2019) and World Health Organization obesity guidelines: sarcopenic obesity (SO, n = 20), sarcopenic without obesity (Sar, n = 18), and control (Con, n = 22). The gut microbiota was analyzed by sequencing the V3-V4 regions of the 16S rRNA gene. Results Significant differences in the diversity and composition of the gut microbiota were observed in Sar and SO groups. A reduction in alpha diversity, as measured by Chao1 and ACE index, was found in the SO group. Beta diversity based on unweighted Unifrac was significantly different between SO, Sar, and Con groups. Bacterial community composition analysis showed that at the family and genus level, the relative abundance of Rikenellaceae, Christensenellacea, Alistipes, Christensenellaceae R-7 group, Ruminococcus, and Odoribacter were significantly abundant in Sar group, whereas Enterobacteriaceae was more enriched in the SO group. Furthermore, as revealed by linear discriminant analysis effect size (LEfSe) and receiver operating curve (ROC) analysis, Alistipes and Christensenellaceae R-7 group was identified as biomarker for the diagnosis of sarcopenia without obesity, and Enterobacteriaceae was identified as a biomarker for the diagnosis of sarcopenic obesity. Conclusion This study demonstrated that the gut microbiota of sarcopenic obesity and sarcopenia without obesity have distinct diversity and composition profiles. The results provide new insights into the role of the gut microbiota in sarcopenic obesity, highlighting the potential of gut microbiota as a therapeutic target in this condition.

show abstract

Section: Discussionsupporting

confidence: 91%

Distinct Gut Microbiota Signatures in Order People with Sarcopenia without Obesity and Sarcopenic Obesity

Wang,

He,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…To examine whether the migration ability of the AIEC LF82 strain was enhanced, the swimming motility of the bacterium was measured using Luria Bertani (LB) medium containing 0.3% agar [ 9 ]. The swimming diameters of the AIEC LF82 strain were significantly greater than those of the non-AIEC K12 strain (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The bacterial invasion is promoted by the binding of bacterial outer membrane proteins ( OmpA ) to the ER-stress response chaperone, Gp96, which is strongly expressed on the apical surface of ileal epithelial cells [ 7 , 8 ]. The specific adhesion and invasiveness of AIEC are believed to strongly contribute to the development and pathogenesis of CD, especially the postoperative recurrence [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Cyclic-di-AMP confers an invasive phenotype on Escherichia coli through elongation of flagellin filaments

Tanaka,

Imai,

Sugiyama

et al. 2024

Gut Pathog

View full text Add to dashboard Cite

Background Adherent-invasive Escherichia coli (AIEC) is isolated from patients with Crohn’s disease (CD). AIEC can invade the intestinal epithelium, suggesting that it is involved in the development and pathogenesis of CD. However, the mechanism by which AIEC acquired the invasive phenotype remains unknown. Results This study was designed to examine the mechanisms of AIEC invasiveness. We found that the flagellin (fliC) expression in AIEC was two-fold higher than that in non-AIEC strains, and this overexpression induced the formation of long-filament flagellin. Deletion of fliC in the AIEC LF82 strain resulted in the disappearance of flagellar filaments and attenuated the motility and invasive ability of the bacterium, suggesting that the formation of long filament flagellin induced by increased fliC expression is required by AIEC to invade the intestinal epithelium. In AIEC and non-AIEC K12 strains cultured in the presence of cyclic-di-AMP (c-di-AMP), the expression of fliC was enhanced, and flagellar filaments were elongated. Stimulation with c-di-AMP enhanced the bacterial motility and ability to invade epithelial cells, even in the non-AIEC K12 strain. Conclusions Our findings show that c-di-AMP confers an AIEC-like phenotype on non-AIEC strains by enhancing the expression of fliC. The results should be useful for understanding the pathogenesis of CD.

show abstract

“…To examine whether the migration ability of the AIEC LF82 strain was enhanced, the swimming motility of the bacterium was measured using LB medium containing 0.3% agar (10). The swimming diameters of the AIEC LF82 strain were signi cantly greater than those of the non-AIEC K12 strains (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The speci c adhesion and invasiveness of AIEC is belived to strongly contribute to the development and pathogenesis of CD, especially the postoperative recurrence (10).…”

Section: Introductionmentioning

confidence: 99%

Cyclic-di-AMP confers an invasive phenotype on Escherichia coli through elongation of flagellin filaments

Tanaka,

Imai,

Sugiyama

et al. 2023

Preprint

View full text Add to dashboard Cite

Adherent-invasive Escherichia coli (AIEC) is isolated from patients with Crohn’s disease (CD). AIEC can invade the intestinal epithelium, suggesting that it is involved in the development and pathogenesis of CD. However, themechanism by which AIEC acquired the invasive phenotype remains unknown. This study was designed to examine the mechanisms of AIEC invasiveness. We found that the flagellin (fliC) expression in AIEC was two-fold higher than that in non-AIEC strains, and this overexpression induced the formation of long-filament flagellin. Deletion of fliC in the AIEC LF82 strain resulted in the disappearance of flagellar filamentsand attenuated the motility and invasive ability of the bacterium, suggesting that the formation of long filament flagellin induced by increased fliC expression is required by AIEC to invade the intestinal epithelium. In AIEC and non-AIEC K12 strains cultured in the presence of cyclic-di-AMP (c-di-AMP), the expression of fliC was enhanced, and flagellar filaments were elongated. Stimulation with c-di-AMP enhanced the bacterial motility and ability to invade epithelial cells, even in the non-AIEC K12 strain. Our findings show that c-di-AMP confers an AIEC-like phenotype on non-AIEC strains by enhancing the expression of fliC. The results should be useful for understanding the pathogenesis of CD.

show abstract

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

Cited by 13 publications

References 138 publications

Distinct Gut Microbiota Signatures in Order People with Sarcopenia without Obesity and Sarcopenic Obesity

Distinct Gut Microbiota Signatures in Order People with Sarcopenia without Obesity and Sarcopenic Obesity

Cyclic-di-AMP confers an invasive phenotype on Escherichia coli through elongation of flagellin filaments

Cyclic-di-AMP confers an invasive phenotype on Escherichia coli through elongation of flagellin filaments

Contact Info

Product

Resources

About