Identification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018

Seyfi, Bahareh; Hossainpour, Hadi; Kooti, Sara; Jalilian, Farid Azizi

doi:10.30699/ijmm.16.4.357

Cited by 4 publications

(3 citation statements)

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“…Cockroaches and houseflies might act as vectors that disseminate MDR to humans via direct contact, human food contamination, and horizontal gene transfer [45]. A couple of very recent reports from Iran [17] and Spain [19] showed the importance of B. germanica as reservoirs and transmission vectors for MDR pathogens because they form the main threat to global health nowadays, and annually, a lot of patients die in hospitals due to infections with MDR bacteria, especially Staphylococcus aureus [18]. So, the irrational use of antibiotics needs to be banned and/or restricted in both human and animal medicine prescriptions to prevent the emergence of MDR bacterial species in the community.…”

Section: Proteusmentioning

confidence: 99%

“…In Ethiopia, however, food-borne illness was found to be associated with five bacterial species from the gut of cockroaches [15]. Recently, antibiotic resistance properties and distribution of virulence factors in the pathogenic bacteria isolated from hospital cockroaches have been reported in Iran [16,17], Saudi Arabia [18], and Spain [19].…”

Section: Introductionmentioning

confidence: 99%

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Antibiogram and MDR Pattern of the Bacterial Isolates from German Cockroaches (<i>Blattella germanica</i> L.) at RMCH, Rajshahi, Bangladesh

Akter,

Islam,

Mohanta

et al. 2023

J. Sci. Res.

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German cockroaches, Blattella germanica L. are the most dangerous pests in hospital environments which cause diseases like food poisoning, dysentery, and diarrhea. Antibiogram and multidrug resistance (MDR) patterns of the bacterial isolates from the cockroaches inhabiting three Wards of Rajshahi Medical College Hospital, Rajshahi, Bangladesh, were determined. A total of 52 bacterial isolates, 26 from the cuticle and 26 from the gut, comprising 20 (38.5 %) from the Neuromedicine ward, 18 (34.6 %) from the Orthopaedic ward, and 14 (26.9 %) from the Surgery ward, were used for biochemical assays and diagnostic characteristics, where seven Gram-negative rod species were identified. E. coli (n=8 or 40 %), Klebsiella spp. (n=5 or 28 %) and Klebsiella spp. (5 or 35 %) constituted the most dominant bacteria from the three wards respectively. Antibiogram of the bacterial isolates against 18 commonly used antibiotics revealed that Serratia, Klebsiella, Escherichia, and Pseudomonas were resistant to Ceftazidime, whereas Salmonella, Enterobacter, and Proteus were sensitive to the drug. Results of the present study suggest that the German cockroaches might serve as reservoirs for pathogenic and MDR bacteria, which in turn could be responsible for the spread of common food-borne diseases in hospital patients, their attendants, and health professionals.

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Section: Proteusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibiogram and MDR Pattern of the Bacterial Isolates from German Cockroaches (<i>Blattella germanica</i> L.) at RMCH, Rajshahi, Bangladesh

Akter,

Islam,

Mohanta

et al. 2023

J. Sci. Res.

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“…Carbapenems generally have the broadest spectrum of activities and the highest potency against all GNB and Gram-positive bacteria ( 2 ). These antibiotics are the last resort for patients who are severely ill or suspected of being infected with ESBL and AmpC-resistant bacteria ( 3 ) In recent years, resistance to carbapenems has been increasingly reported in different countries ( 4 , 5 ). The main resistance mechanisms against carbapenems are as follows: 1) reduction of permeability; 2) efflux pumps; 3) changes in Penicillin-Binding Protein (PBP); and 4) ß-lactamases production ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Molecular Characterization of Carbapenems Resistant Escherichia coli Isolated from Patients with Urinary Tract Infections in Ardabil Province, Iran

et al. 2022

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Scan to discover onlineBackground & Objective: Carbapenem-resistant is Gram-negative bacteria representing a worldwide public health problem. The present study aims to survey the phenotypic and genotypic characteristics of carbapenem-resistant Escherichia coli isolates collected from hospitalized patients and outpatients in Ardabil province, Iran.Methods: Two hundred samples were collected from the patients who had already been referred to the hospitals in Ardabil, Iran, from January to June 2017. Each patient's social and demographic data were recorded in the first step. The resistance profile of all E. coli isolates against imipenem and meropenem antibiotics were determined using the Kirby-Bauer disk diffusion method. Moreover, the broth microdilution method determined the Minimum Inhibitory Concentration (MIC) of E. coli isolates to imipenem. The Carbapenem Inactivation Method (CIM) and Carba NP test were employed for screening carbapenem-resistant strains. The frequency of carbapenemencoding genes was determined using Polymerase Chain Reaction (PCR) method. The Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR analysis was used to evaluate the genetic relatedness of E. coli isolates.Results: Out of 200 urine samples, 66% (n = 132) of the samples were collected from women. The patients' age varied from 1 month to 93 years. Results of the disk diffusion method revealed that 33% (n=66/200) of E. coli isolates were resistant to imipenem. However, imipenem resistance was detected in 37% (n = 74/200) of the E. coli isolates using broth microdilution method. All E. coli isolates were negative in CIM and Carba NP tests. Moreover, we could not detect any carbapenemase encoding genes among E. coli isolates. The ERIC-PCR method revealed the E. coli strains were classified into 39 clusters with 80% similarity.Conclusion: It appears that E. coli is the most common cause of urinary tract infection in Ardabil province.

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Extended spectrum and metalo beta lactamase producing gram negative bacterial pathogens from cockroaches collected at hospital, Southern Ethiopia

Bisetegn,

Azene,

Ahmed

et al. 2024

Antimicrob Resist Infect Control

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Background Cockroaches can pose a significant health risk in hospital environments because they may serve as reservoirs and vectors for nosocomial pathogens. Cockroaches harbor epidemiologically significant extended spectrum and metalo beta lactamase producing Gram negative bacterial pathogens, which complicate nosocomial infections. Objectives The main aim of this study is to determine aetiology and phenotypic extended spectrum and metalo beta lactamase producing Gram negative bacteria pathogens from cockroaches collected in hospitals. Methods A cross-sectional study was employed from February to May 2022 to determine the antibiotic resistance producing bacterial isolates from cockroaches by giving special emphasis to metalo beta lactamase and extended spectrum beta lactamase production from different wards of WSUCSH. Cockroaches were collected with hands wearing sterile gloves. External homogenate was prepared and incubated microbiologically by using different culture media and differentiated biochemically. Antimicrobial susceptibility testing was performed by disk diffusion method. ESBL production was conducted using double disc synergy method and double disk method was used to detect MBL enzyme detection. Descriptive statistics was used to determine prevalence and percentage. Result Out of 245 cockroaches, 108 Gram negative bacteria were isolated. K. pneumoniae 29(26.9%) was the most predominant bacteria and Enetrobacter spp. 8(7.4%), was the least. All, K. pneumoniae, P. mirabilis, and Enterobacter isolates were pan-resistant to Ampicillin. P.aeruginosa and P.mirabilis antibiotics showed ≥ 80% resistant for amoxicillin/clavulanic acid antibiotics. Cefotaxime, ceftazidime, ceftriaxone and imipenem showed relative efficacy compared with other antibiotics. Out of 78 amoxicillin-clavulanic acid resistant isolates, 42(34.7%) were ESBL producers. ESBL production is more depicted by P. aeruginosa, A. baumannii, K. pneumoniae and E. coli. The overall prevalence of MBL production is 29(23.1%). K. pneumoniae P. aeruginosa, E.coli, A. baumannii, Enterobacter spp and K.oxytoca revealed MBL production. Conclusion The overall prevalence of ESBL and MBL producing nosocomial agents from hospital cockroaches was 34.7% and 23.1% respectively. P.aeruginosa, A.baumannii, K.pneumoniae and E.coli showed pronounced ESBL production. All bacterial isolates except P. mirabilis and C. freundii showed MBL production. The needed to evaluate our antibiotic stewardship program and antibiotic resistance detection for treatment is mandatory. The impact of cockroach as a source of AMR should be sought.

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Identification of Carbapenem Resistance Genes in Escherichia coli Isolated from Blattella germanica by Dot Blot Assay in Hamadan Hospitals, Iran – 2018

Cited by 4 publications

References 25 publications

Antibiogram and MDR Pattern of the Bacterial Isolates from German Cockroaches (<i>Blattella germanica</i> L.) at RMCH, Rajshahi, Bangladesh

Antibiogram and MDR Pattern of the Bacterial Isolates from German Cockroaches (<i>Blattella germanica</i> L.) at RMCH, Rajshahi, Bangladesh

Phenotypic and Molecular Characterization of Carbapenems Resistant Escherichia coli Isolated from Patients with Urinary Tract Infections in Ardabil Province, Iran

Extended spectrum and metalo beta lactamase producing gram negative bacterial pathogens from cockroaches collected at hospital, Southern Ethiopia

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