Identification of QTLs associated with citrus resistance toPhytophthora gummosis

Siviero, Amauri; Cristofani, M; Furtado, Edson Luiz; Garcia, Antônio Augusto Franco; Coelho, Alexandre Siqueira Guedes; Machado, Marcos Antônio

doi:10.1007/bf03194595

Cited by 46 publications

(36 citation statements)

References 15 publications

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“…Siviero et al (2002) found only one QTL associated with the quantitative inheritance of characteristics (number of fruits per plant and number of seeds per fruit) in a F1 progeny derived from a cross between Citrus sunki and Poncirus trifoliata 'Rubidoux'. A study on root-rot resistance caused by Phytophthora in progeny derived from a cross between C. sunki and P. trifoliata (Siviero et al, 2006) provided two QTL maps for the species P. trifoliate, which could explain 16-24% of the genetic variation. In addition, only one QTL was mapped for the species C. sunki, which could only account for 14% of the variation.…”

Section: Genetics and Molecular Research 15 (4): Gmr15048863mentioning

confidence: 99%

“…However, the identification of QTL associated with such characteristics only explains a small fraction of the observed genetic variation in each character (Siviero et al, 2002;Siviero et al 2006;Gussen et al, 2011;Asins et al, 2012); therefore, selection based on these markers is not feasible. The rationale for not using MAS is that traits under selection are governed by many loci with small effects that do not reach statistical significance when used in traditional QTL analysis, and this in turn leads to the identification of only a small number of QTL with major effects (Kemper and Goddard 2012;Resende et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Genome wide selection in Citrus breeding

et al. 2016

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ABSTRACT. Genome wide selection (GWS) is essential for the genetic improvement of perennial species such as Citrus because of its ability to increase gain per unit time and to enable the efficient selection of characteristics with low heritability. This study assessed GWS efficiency in a population of Citrus and compared it with selection based on phenotypic data. A total of 180 individual trees from a cross between Pera sweet orange (Citrus sinensis Osbeck) and Murcott tangor (Citrus sinensis Osbeck x Citrus reticulata Blanco) were evaluated for 10 characteristics related to fruit quality. The hybrids were genotyped using 5287 DArT_seq TM (diversity arrays technology) molecular markers and their effects on phenotypes were predicted using the random regression -best linear unbiased predictor (rr-BLUP) method. The predictive ability, prediction bias, and accuracy of GWS were estimated to verify its effectiveness for phenotype prediction. The proportion of genetic variance explained by the markers was also computed. The heritability of the traits, as determined by markers, was 16-28%. The predictive ability of these markers ranged from 0.53 to 0.64, and the regression coefficients between predicted and observed phenotypes were close to unity. Over 35% of the genetic variance was accounted for by the markers. Accuracy estimates with GWS were lower than those obtained by phenotypic analysis; however, GWS was superior in terms of genetic gain per unit time. Thus, GWS may be useful for Citrus breeding as it can predict phenotypes early and accurately, and reduce the length of the selection cycle. This study demonstrates the feasibility of genomic selection in Citrus.

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Section: Genetics and Molecular Research 15 (4): Gmr15048863mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome wide selection in Citrus breeding

et al. 2016

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“…These results confirm that Poncirus trifoliata is highly resistant, the Sour orange as resistant, the Rangpur lime as moderately resistant and Caipira sweet orange and Sunki mandarin as susceptible (Alvarez et al, 2009). According Siviero et al (2006), the lesions length is more suitable to characterize the rootstocks and new genotypes resistance to Phytophthora spp. when compared with measurements of the width of injury.…”

Section: Effect Of Organic Manure In the Control Of P Parasitica In mentioning

confidence: 99%

Effect of Organic Manure in the Induction of Resistance of Citrus to Phytophthora parasitica

Cristofani–Yaly¹,

Boava²,

Schinor³

et al. 2015

JAS

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Gummosis and root rot caused by Phytophthora are among the most economically important diseases in Citrus. Chemical control has been used; however, it is essential to search for new alternatives for its control. This study aimed to: (i) produce an organic compound from the anaerobic fermentation of cattle manure, (ii) determine the microbial diversity present in the compound, (iii) determine the effect of compound on mycelial growth of Phytophthora parasitica (P. nicotianae), (iv) evaluate its effect in inducing resistance to the pathogen in five Citrus rootstocks and, (v) evaluate the expression profile of some defense-related genes in resistant plants of Poncirus trifoliata and susceptible plants of Caipira sweet orange. The largest microbial population occurred between 25 and 35 days after production of organic manure with predominance of Bacillus spp. The organic manure at concentration of 20% inhibited about 80% of the mycelial growth of P. parasitica. Plants of Caipira sweet orange (susceptible to P. parasitica) when treated with organic manure at all concentrations (10, 20 and 50%), had lower disease severity when compared with the respective control. The analysis of gene expression suggested that the combination of treatment followed by inoculation with the pathogen in plants of Caipira sweet orange, the susceptible genotype, was able to increase the expression level of the genes encoding chitinase (CHI), lipoxygenase (LOX), peroxidase (POX), β-1,3-glucanase and chalcone synthase (CHS). It is believed that they should be involved with the resistance of Citrus plants to P. parasitica.

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“…It has been widely used in citriculture in China, Japan, and many other countries for its superior performances such as resistance to cold (Young 1977), Phytophthora root rot, nematode (O'Bannon 1977), citrus tristeza virus (Gmitter et al 1996), and gummosis (Siviero et al 2006). In addition, trifoliate orange produces large number of seeds with nucellar embryos, which are genetically identical to the mother plant.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Poncirus and phylogenetic relationships with its relatives revealed by SSR and SNP/InDel markers

et al. 2015

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The polymorphic markers of nuclear SSR (simple sequence repeat), SNP (single nucleotide polymorphism)/InDel (insertion-deletion length polymorphism), and cpSSR (chloroplast SSR) were used to evaluate the genetic diversity of 52 trifoliate oranges and their relatives. The two genomic DNA-based markers produced high values of PIC (polymorphic information content) at 0.45 for SNP/InDel and 0.32 for nuclear SSR, indicating that there is a large diversity of genetic variation within the trifoliate oranges. Three UPGMA (unweighted pair-group method with arithmetic means) dendrograms were generated based on the results of three marker systems. At 0.76 of coefficient, all of Poncirus accessions were clustered into one group, except the 74-1 Early Poncirus, one of the three precocious trifoliate oranges, which was placed separately into another group by nuclear SSR. At a higher coefficient, the trifoliate oranges were discriminated into 4, 6 and 6 groups by nuclear SSR (0.86), SNP/InDel (0.95), and cpSSR (0.95), respectively. The results revealed that three precocious trifoliate oranges were originated from different sources. Hubei Early Poncirus and Beibei Early Poncirus were the asexual mutants of different trifoliate oranges, and 74-1 was probably a sexual hybrid. The present study also indicated that 'Zhiwenzhou,' a diploid chimera of trifoliate orange and Satsuma is more closely related to Satsuma rather than to trifoliate orange, which is in concordance with the results of morphological observation.

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Identification of QTLs associated with citrus resistance toPhytophthora gummosis

Cited by 46 publications

References 15 publications

Genome wide selection in Citrus breeding

Genome wide selection in Citrus breeding

Effect of Organic Manure in the Induction of Resistance of Citrus to Phytophthora parasitica

Genetic diversity of Poncirus and phylogenetic relationships with its relatives revealed by SSR and SNP/InDel markers

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