Identification of Pseudomonas fluorescens using different biochemical tests

Nepali, Bikram; Bhattarai, Sabin; Shrestha, Jiban

doi:10.20956/ijab.v2i2.5260

Cited by 10 publications

(8 citation statements)

References 5 publications

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“…The physiological characterization of bacteria was carried out by several tests, including: the gram test using 3% KOH solution and bacterial gram staining (Mu'minah et al 2015;Begum et al 2017); the oxidase test used Sigma strip oxidase contains plastic strips with a paper zone saturated with a solution of N,N-dimethyl-1,4phenylene diamine and alpha-naphthol, catalase test (Sudewi et al 2020); oxidation fermentation test, tolerance test for temperature conditions of 37-40°C and acid using 5% NaCl, fried egg colony test on PDA media (Thakkar et al 2016); sensitivity test to 15 µg erythromycin antibiotic (Kanzil et al 2015), and fluorescent pigment production using King's B media (Nepali et al 2018). In addition, enzyme activity tests were conducted on the ability of bacteria to produce enzymes: protease, lecithinase, and pectolytic enzymes, following the methods of Boluk et al (2021), Thakur et al (2021) and Kumvinit and Akarapisan (2019).…”

Section: Identification Of the Bacterial Isolate Based On Physiologic...mentioning

confidence: 99%

Morpho-physiological and molecular characteristics of bacteria causing stalk rot disease on corn in Gorontalo, Indonesia

et al. 2023

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Abstract. Suriani, Patandjengi B, Muis A, Junaid M, Mirsam H, Azrai M. 2023. Morpho-physiological and molecular characteristics of bacteria causing stalk rot disease on corn in Gorontalo, Indonesia. Biodiversitas 24: 1749-1758. Stalk rot disease was observed in corn in Gorontalo with typical symptoms, such as soft rot on the stalk, leaf wilting, and plant death. This study aimed to characterize the bacteria causing stalk rot disease in corn. Samples of infected plants were collected and identified morphologically, physiologically, and molecularly. The results showed that nine bacterial isolates were isolated from infected plants. All nine isolates showed positive hypersensitive responses on tobacco leaves. In comparison, only two bacterial isolates (BGO1 and BGO4) were positive on pathogenicity tests on corn. However, the BGO4 isolate caused the highest disease incidence with a faster incubation period. The BGO4 isolate was gram-negative with white-gray colored colonies. Physiological characterization of BGO4 also showed: positive catalase and indole, oxidase negative, fermentative oxidation, caused soft rot on potato, non-fluorescent, and sensitive to erythromycin. In addition, it can grow at 37-40°C and 5% NaCl, producing protease and lecithinase enzymes. The BGO4 also isolates infected rice, corn, sorghum, foxtail millet, celery, and Aloe vera. Morpho-physiology characteristics and diagnostic amplification of DNA by PCR using the Dickeya-specific primers (ADE1/ADE2) showed that the isolate belongs to the genus Dickeya. Further molecular characterization by analysis of the 16S rDNA using universal primer 27F/1497R successfully amplified the DNA band of BGO4 isolate measuring ±1300 bp. Phylogenetic analysis showed that it was in the same group as Dickeya zeae strain MS32 from Taiwan, strain DZ15SB01 (Thailand), and strain HNJF02 (China), with the coefficient of genetic distance ranging from 0.001 to 0.002. This study is the first report of D. zeae infecting corn in Gorontalo.

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Section: Identification Of the Bacterial Isolate Based On Physiologic...mentioning

confidence: 99%

Morpho-physiological and molecular characteristics of bacteria causing stalk rot disease on corn in Gorontalo, Indonesia

et al. 2023

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“…demonstrate a vast metabolic diversity, which often limits their discrimination through biochemical approaches. For example, while some species, such as P. syringae and P. viridiflava, show a negative reaction in the oxidase test, most species, including P. fluorescens, give a positive result [16]. In fact, LOPAT tests are not able to differentiate Psa from, e.g., P. syringae pv.…”

Section: Pathogen Detection and Identificationmentioning

confidence: 99%

Mitigation of Emergent Bacterial Pathogens Using Pseudomonas syringae pv. actinidiae as a Case Study—From Orchard to Gene and Everything in Between

Silva

Santos

Vasconcelos

et al. 2022

Crops

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Globalization propelled human migration and commercial exchanges at the global level, but woefully led to the introduction of non-indigenous organisms into several agroecological systems. These include pathogenic bacteria with devastating consequences for numerous crops of agronomical importance for food production worldwide. In the last decade, research efforts have focused on these noxious organisms, aiming to understand their evolutionary processes, degree of pathogenicity, and mitigation strategies, which have allowed stakeholders and policymakers to develop evidence-based regulatory norms to improve management practices and minimize production losses. One of these cases is the bacterium Pseudomonas syringae pv. actinidiae (Psa), the causal agent of the kiwifruit bacterial canker, which has been causing drastic production losses and added costs related to orchard management in the kiwifruit industry. Although Psa is presently considered a pandemic pathogen and far from being eradicated, the implementation of strict regulatory norms and the efforts employed by the scientific community allowed the mitigation, to some extent, of its negative impacts through an integrated pest management approach. This included implementing directive guidelines, modifying cultural practices, and searching for sources of plant resistance. However, bacterial pathogens often have high spatial and temporal variability, with new strains constantly arising through mutation, recombination, and gene flow, posing constant pressure to agroecosystems. This review aims to critically appraise the efforts developed to mitigate bacterial pathogens of agronomical impact, from orchard management to genome analysis, using Psa as a case study, which could allow a prompter response against emerging pathogens in agroecosystems worldwide.

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“…Gelatinase will break down gelatin into amino acids, which will be used in the growing process. Hydrolyzed gelatin cannot form gel and turns liquid once taken out from a refrigerator 23 . The gelatin hydrolysis test results of the three bacterial isolates were positive.…”

Section: Biochemical Characters Of the Bacterial Isolatesmentioning

confidence: 99%

Phenotypic Characterization and Identification of Potential L-Asparaginase-Producing Thermohalophilic Bacteria from Wawolesea Hot Spring, North Konawe, Southeast Sulawesi, Indonesia

Muzuni¹,

Suriana²,

Yanti³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: L-asparaginase-producing thermohalophilic bacteria have the potential of producing an enzyme tolerant to high heat and salt levels. This enzyme, L-asparaginase, can be used as a biological agent for the cancer therapy of acute lymphoblastic leukemia and melanosarcoma as it has a specific ability to inhibit the formation of nutrients for cancer cells. This enzyme is also used effectively in food industries operating at high temperatures due to its ability to reduce acrylamide, a trigger of cancer cells. This study sought to figure out the phenotypic characters of and identify potential L-asparaginase-producing thermohalophilic bacteria from Wawolesea Hot Spring, North Konawe, Southeast Sulawesi. Materials and Methods: The characterization conducted on potential L-asparaginase-producing thermohalophilic bacterial isolates consisted of the following: Colony morphological characterization, covering the shapes, edges, internal structures, elevations and colours of the colonies, cell morphological characterization, covering gram staining, endospore formation and motility, biochemical characterization, covering catalase, Methyl Red and Voges Proskauer (MR-VP), gelatin hydrolysis, citrate, indole and carbohydrate fermentation tests and physiological characterization, covering pH effect, salinity, oxygen demand and temperature effect tests. Bacterial isolate identification was carried out in two stages, namely phenetic identification based on the phenotypic characterization data determine through a preliminary identification and numeric-phenetic identification. Results:The characterization results showed that the bacterial isolates AAT 1.4, AAT 3.2 and CAT 3.4 were bacillus-shaped, Gram-positive, motile, catalase-positive and aerobic. Based on the numeric-phenetic analysis results, the isolates AAT 1.4 and CAT 3.4 had a 92.9% similarity to Bacillus subtilis, while isolate AAT 3.2 had a 92.9% similarity to Brevibacillus limnophilus. Conclusion: According to the numeric-phenetic analysis results, the isolates AAT 1.4 and CAT 3.4 belong to the species Bacillus subtilis, while isolate AAT 3.2 belongs to the species Brevibacillus limnophilus.

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Identification of Pseudomonas fluorescens using different biochemical tests

Cited by 10 publications

References 5 publications

Morpho-physiological and molecular characteristics of bacteria causing stalk rot disease on corn in Gorontalo, Indonesia

Morpho-physiological and molecular characteristics of bacteria causing stalk rot disease on corn in Gorontalo, Indonesia

Mitigation of Emergent Bacterial Pathogens Using Pseudomonas syringae pv. actinidiae as a Case Study—From Orchard to Gene and Everything in Between

Phenotypic Characterization and Identification of Potential L-Asparaginase-Producing Thermohalophilic Bacteria from Wawolesea Hot Spring, North Konawe, Southeast Sulawesi, Indonesia

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