Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

Luo, Yongli; Pang, Dangwei; Jin, Min; Chen, Jin; Kong, Xiang Bo; Li, Wenqian; Chang, Yajie; Li, Yong; Wang, Zhenlin

doi:10.1002/pld3.152

Cited by 30 publications

(15 citation statements)

References 67 publications

(91 reference statements)

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“…It is important to screen premature aging genes and explore the causes and mechanisms of premature aging. It was found [44] that water deficit during grain filling period could cause premature senescence of flag leaves, but the senescence process could be delayed by changing hormone concentration of plants. There are many reasons for rice premature aging, for example, the effect of NAC transcription factors on abscisic acid (ABA) [25], the functional impairment of calcium-dependent protein kinase OsCPK12 [28], the deletion of gene fragment [29], the response and regulation of genes related to antioxidant and carbohydrate metabolism [45], and so on.…”

Section: Discussionmentioning

confidence: 99%

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

et al. 2020

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Background: Lesion-mimic and premature aging (lmpa) mutant lmpa1 was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis of lmpa1 mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out in lmpa1, KD527 and their F 1 and F 2 derivatives. Further, bulked segregation analysis (BSA) in combination with a 660 K SNP array were conducted on the F 2 segregation population of lmpa1/Chinese spring (CS) to locate the lmpa1 gene. Results: Most agronomic traits of lmpa1 were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F 1 plants from the crossing of lmpa1 and KD527 exhibited the lmpa phenotype and the F 2 progenies showed a segregation of normal (wild type, WT) and lmpa, with the ratios of lmpa: WT = 124:36(χ 2 = 1.008 < =3.841), indicating that lmpa is a dominant mutation. The combination of BSA and the SNP array analysis of CS, lmpa1 and lmpa1/CS F 2 WT pool (50 plants) and lmpa pool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging gene Lmpa1 was probably located on the short arm of chromosome 5A. Conclusions: EMS-mutagenized mutant lmpa1 deriving from elite wheat line KD527 conferred lmpa. Lmpa phenotype of lmpa1 mutant is controlled by a single dominant allele designated as Lmpa1, which affected wheat growth and development and reduced the thousand grain weight (tgw) of single plant in wheat. The gene Lmpa1 was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.

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Section: Discussionmentioning

confidence: 99%

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

et al. 2020

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“…It is important to screen premature aging genes and explore the causes and mechanisms of premature aging. It was found [37] that water deficit during grain filling period could cause premature senescence of flag leaves, but the senescence process could be delayed by changing hormone concentration of plants. There are many reasons for rice premature aging, for example, the effect of NAC transcription factors on abscisic acid (ABA) [19], the functional impairment of calcium-dependent protein kinase OsCPK12 [22], the deletion of gene fragment [23], the response and regulation of genes related to antioxidant and carbohydrate metabolism [38], and so on.…”

Section: Discussionmentioning

confidence: 99%

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

Kong

Wang

Cao

et al. 2020

Preprint

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Background: Lesion-mimic and premature aging ( lmpa ) mutant lm pa 1 was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis of l mpa 1 mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out in l mpa 1 , KD527 and their F 1 and F 2 derivatives. Further, bulked segregation analysis (BSA) in combination with a 660K SNP Chip were conducted on the F 2 segregation population of l mpa 1 /Chinese spring(CS) to locate the l mpa 1 gene. Results: Most agronomic traits of l mpa 1 were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F 1 plants from the crossing of l mpa 1 and KD527 exhibited the l mpa phenotype and the F 2 progenies showed a segregation of normal (wild type, WT) and l mpa , with the ratios of l mpa :WT=124:36（χ 2 =1.008<=3.841), indicating that l mpa is a dominant mutation. The combination of BSA and the SNP Chip analysis of CS, l mpa 1 and l mpa 1 /CS F 2 WT pool (50 plants) and l mpa pool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging gene Lmpa1 was probably located on the short arm of chromosome 5A. Conclusions: EMS-mutagenized mutant lmpa 1 deriving from elite wheat line KD527 conferred lmpa . lmpa phenotype of l mpa 1 mutant is controlled by a single dominant allele designated as Lmpa1 , which affected wheat growth and development and reduced the thousand grain weight ( tgw ) of single plant in wheat. The gene Lmpa1 was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.

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“…Moreover, cisZOGT1, a cis -zeatin O -glucosyltransferase, is involved in wheat leaf senescence by regulating CK and N metabolism (Wang et al, 2019 ). By the high-throughput analysis, researchers have also identified some candidate genes in drought-induced leaf senescence in wheat (Luo et al, 2019 ). Meanwhile, TaSCL14 is a member of the GRAS protein family in wheat and plays multiple roles in development, photosynthesis, stress response, and dark-induced senescence (Chen et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

et al. 2021

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Leaf senescence is crucial for crop yield and quality. Transcriptional regulation is a key step for integrating various senescence-related signals into the nucleus. However, few regulators of senescence implicating transcriptional events have been functionally characterized in wheat. Based on our RNA-seq data, we identified a WRKY transcription factor, TaWRKY13-A, that predominately expresses at senescent stages. By using the virus-induced gene silencing (VIGS) method, we manifested impaired transcription of TaWRKY13-A leading to a delayed leaf senescence phenotype in wheat. Moreover, the overexpression (OE) of TaWRKY13-A accelerated the onset of leaf senescence under both natural growth condition and darkness in Brachypodium distachyon and Arabidopsis thaliana. Furthermore, by physiological and molecular investigations, we verified that TaWRKY13-A participates in the regulation of leaf senescence via jasmonic acid (JA) pathway. The expression of JA biosynthetic genes, including AtLOX6, was altered in TaWRKY13-A-overexpressing Arabidopsis. We also demonstrated that TaWRKY13-A can interact with the promoter of AtLOX6 and TaLOX6 by using the electrophoretic mobility shift assay (EMSA) and luciferase reporter system. Consistently, we detected a higher JA level in TaWRKY13-A-overexpressing lines than that in Col-0. Moreover, our data suggested that TaWRKY13-A is partially functional conserved with AtWRKY53 in age-dependent leaf senescence. Collectively, this study manifests TaWRKY13-A as a positive regulator of JA-related leaf senescence, which could be a new clue for molecular breeding in wheat.

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Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

Cited by 30 publications

References 67 publications

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

TaWRKY13-A Serves as a Mediator of Jasmonic Acid-Related Leaf Senescence by Modulating Jasmonic Acid Biosynthesis

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