Identification and genetic analysis of EMS-mutagenized wheat mutants conferring lesion-mimic premature aging

Abstract

Background: Lesion-mimic and premature aging (lmpa) mutant lmpa1 was identified from the ethyl methane sulfonate (EMS) mutant library in the bread wheat variety Keda 527 (KD527) background. To reveal the genetic basis of lmpa1 mutant, phenotypic observations and analyses of chlorophyll content and photosynthesis were carried out in lmpa1, KD527 and their F 1 and F 2 derivatives. Further, bulked segregation analysis (BSA) in combination with a 660 K SNP array were conducted on the F 2 segregation population of lmpa1/Chinese spring (CS) to locate the lmpa1 gene. Results: Most agronomic traits of lmpa1 were similar to those of KD527 before lesion-like spots appeared. Genetic analysis indicated that the F 1 plants from the crossing of lmpa1 and KD527 exhibited the lmpa phenotype and the F 2 progenies showed a segregation of normal (wild type, WT) and lmpa, with the ratios of lmpa: WT = 124:36(χ 2 = 1.008 < =3.841), indicating that lmpa is a dominant mutation. The combination of BSA and the SNP array analysis of CS, lmpa1 and lmpa1/CS F 2 WT pool (50 plants) and lmpa pool (50 plants) showed that polymorphic SNPs were enriched on chromosome 5A, within a region of 30-40 Mb, indicating that the wheat premature aging gene Lmpa1 was probably located on the short arm of chromosome 5A. Conclusions: EMS-mutagenized mutant lmpa1 deriving from elite wheat line KD527 conferred lmpa. Lmpa phenotype of lmpa1 mutant is controlled by a single dominant allele designated as Lmpa1, which affected wheat growth and development and reduced the thousand grain weight (tgw) of single plant in wheat. The gene Lmpa1 was tentatively located within the region of 30-40 Mb near to the short arm of chromosome 5A.

Agronomic Characterization of an Ems-Mutagenized Population for Selecting High-Yielding and Glutenenriched Industrial Wheat

“…5) Therefore, our developed mutant lines will play a vital role in meeting the demand for higher productivity and enhanced grain qualities that would also be suitable for industrial purposes. The creation of mutant lines of wheat using EMS has been carried out for a long time, and developing such mutant lines has not only proven to be effective for breeding purposes (Sakin et al, 2005) but also plays a vital role in forward and reverse genetic studies (Chen et al, 2012;Kong et al, 2020).…”

“…Among the various mutation breeding techniques; Physical, chemical, or biological agents (Roy Chowdhury and Tah, 2013), chemical mutagenesis is comparatively a more feasible approach, as it is milder to crops and does not require any kind of special machines (Oladosu et al, 2016). Apart from this different chemical used to generate mutation in the plant, EMS (ethyl methanesulphonate) is the most convenient chemical to improve agronomic characteristics in plants since it induces a higher point mutation and produces no chromosomal aberrations (Kong et al, 2020).…”

Characterization of yellow‐green leaf1 mutant for improved phosphorus use efficiency and rapid offspring screening in Tartary buckwheat

“…Leaf color mutants not only provide valuable genetic resources for exploring the regulatory mechanisms of leaf pigments and photosynthesis but also serve as effective tools for efficient screening of offspring in crop breeding (W. . The yellow (yellow-green) leaf phenotype often occurs in chloroplast-deficient mutants or prematurely aging mutants with reduced chlorophyll content (G. Chen et al, 2005; W. Kong et al, 2020). However, most yellow leaf mutants in crops exhibit impaired growth and low yield, which limits their application in rapid screening of offspring, such as rice yl3 (X.…”

“…Leaf color mutants not only provide valuable genetic resources for exploring the regulatory mechanisms of leaf pigments and photosynthesis but also serve as effective tools for efficient screening of offspring in crop breeding (W. Li et al., 2022). The yellow (yellow‐green) leaf phenotype often occurs in chloroplast‐deficient mutants or prematurely aging mutants with reduced chlorophyll content (G. Chen et al., 2005; W. Kong et al., 2020). However, most yellow leaf mutants in crops exhibit impaired growth and low yield, which limits their application in rapid screening of offspring, such as rice yl3 (X. Liu et al., 2021), ypd1 (D. Chen et al., 2020), mps1 (Z. Liu et al., 2016), ygl6 (Shi et al., 2015), ygl8951 (Chu et al., 2016), and w390 (Dong et al., 2015), maize ygl‐1 (Guan et al., 2016), oil yellow leaf 2 (Yuan et al., 2021), tomato ( Solanum lycopersicum L.) ym (M. Cheng et al., 2022), cabbage ( Brassica rapa ssp.…”

A Phosphoproteomics Study of the Soybean root necrosis 1 Mutant Revealed Type II Metacaspases Involved in Cell Death Pathway

“…Different recessive lesion mimic mutations can lead to PCD with or without activation of host defense responses ( Jones and Dangl, 1996 ; Mittler and Lam, 1996 ; Lorrain et al, 2003 ; Bruggeman et al, 2015 ). LMMs have been reported in many plant species including Arabidopsis ( Zhang et al, 2008 ), barley ( Jorgensen, 1992 ; Rostoks et al, 2006 ), birch ( Li et al, 2017 ), maize ( Walbot, 1991 ; Gray et al, 1997 ), rice ( Mizobuchi et al, 2002 ; Jiao et al, 2018 ; Zhang et al, 2019 ), soybean ( Kosslak et al, 1996 ), and wheat ( Kong et al, 2020 ). A number of genes regulating the development of LMM phenotypes have been mapped and cloned.…”