Identification of Phenolic Compounds from Hancornia speciosa (Apocynaceae) Leaves by UHPLC Orbitrap-HRMS

Abstract

Apocynaceae is a botanical family distributed mainly in tropical and subtropical regions of the world. In Brazil, they comprise about 90 genera and 850 species, inhabiting various types of vegetation. Within this large botanical family, the genus Hancornia is considered monotypic, with its only species Hancornia speciosa Gomes. Antihypertensive, antidiabetic, and antiviral activities are described for this species. Despite having been the target of some studies, knowledge of its chemical composition is still limited. In this study, the phenolics of H. speciosa leaves were analyzed using ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high-resolution mass spectrometry (HRMS). As a result, 14 compounds were identified viz. protocatechuic acid, catechin, and quercetin, and another 14 were putatively identified viz. B- and C-type procyanidins, while just one compound remained unknown. From the identified compounds, 17 are reported for the first time viz. coumaroylquinic acid isomers and eriodyctiol. The results show that Hancornia speciosa can serve as source of valuable phenolics.

Physicochemical Characterization, Microbiological Quality and Safety, and Pharmacological Potential of Hancornia speciosa Gomes

“…The identified chemical constituents that were related to the biological activities include L-(+)-bornesitol, quinic acid, chlorogenic acid, and kaempferol, quercetin, isoquercetin, rutin, and catechin flavonoids [ 4 , 5 , 11 , 13 , 14 ]. Recently, Bastos et al [ 15 ] identified other phenolic compounds, including caffeic acid, protocatechuic acid isomers, epicatechin, quercetin isomers, type-B and type-C procyanidins, coumaroylquinic acid isomers, phlorizin, phloretin, eriodictyol, luteolin, and apigenin. Natural extracts and phenolic derivatives with pharmacological potential may serve as a safe and cost-effective treatment strategy as an alternative to synthetic drugs [ 16 – 19 ].…”

Ultra‐Performance Liquid Chromatography Hyphenated with Quadrupole‐Orbitrap Mass Spectrometry for Simultaneous Determination of Necine‐Core‐Structure Pyrrolizidine Alkaloids in Crotalaria sessiliflora L. without all Corresponding Standards

“…In the GC–MS method, the LOD is also high, and the precision and stability are poor because of the cyclic ester moiety in the PAs structure, which is easy to decompose when heated at a high temperature (Han et al , ). HPLC–MS has been proven to be a powerful and efficient tool for qualitative and quantitative analysis of PAs due to chromatographic separation performance and unequivocal identification ability (McMaster, 2005; Steinmann and Ganzera, ; Bastos et al , ). Even so, accurate quantification of micro amounts of other PAs in the herb is still missing and difficult owing to most of the PA standards being unavailable.…”

Uses and technological prospects for the mangaba, a native fruit of Brazil

“…These compounds are responsible for color, astringency, aroma, and oxidative stability in plants (Angelo and George, 2007). Bastos et al (2017) identified seventeen compounds described for the first time for the mangaba, of which the hydroxyl fatty acids found support for antibiotic claims and the two phenylpropanoids phloretin and phlorizin and the flavonols quercetin and kaempferol support the proposed antidiabetic properties. The results demonstrate the potential of H. speciosa as a source of valuable phenolics.…”