Identification of Phenanthrene Derivatives in <i>Aerides rosea</i> (Orchidaceae) Using the Combined Systems HPLC–ESI–HRMS/MS and HPLC–DAD–MS–SPE–UV–NMR

Čáková, Veronika; Urbain, Aurélie; Antheaume, Cyril; Rimlinger, Nicole; Wehrung, Patrick; Bonté, Frédéric; Lobstein, Annelise

doi:10.1002/pca.2533

Cited by 22 publications

(11 citation statements)

References 24 publications

(34 reference statements)

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“…Thus, glomerular mesangial cells cultured under high glucose conditions were employed as a model of DN [22,23]. Gigantol is a phenolic substance extracted from plants in the genus Dendrobium [14]. As plants of the Dendrobium genus are commonly being used to treat diabetes mellitus in Traditional Chinese Medicine [26], the current study used the in vitro approach to examine the potential effects of gigantol on the high glucose-induced glomerulus mesangial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the chemical components and pharmacology of Dendrobium plants have been studied to provide scientific proof to justify the medicinal use in the treatment of diseases [13]. Gigantol (3′,4-dihydroxy-3,5′-dimethoxybibenzyl) is a bibenzyl-type phenolic compound isolated from several medicinal orchids, which contains active phenolic hydroxyl groups, displays aromatic and hydrophobic characteristics, and exerts a wide range of pharmacological activities [14]. Previous studies have shown that gigantol has several bioactivities including anti-osmotic [15], antioxidant [15], antispasmodic [16], antinociceptive and anti-inflammatory effects [17].…”

Section: Introductionmentioning

confidence: 99%

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Gigantol has Protective Effects against High Glucose-Evoked Nephrotoxicity in Mouse Glomerulus Mesangial Cells by Suppressing ROS/MAPK/NF-κB Signaling Pathways

et al. 2018

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Gigantol is a bibenzyl compound derived from several medicinal orchids. This biologically active compound has shown promising therapeutic potential against diabetic cataracts, but whether this compound exerts beneficial effects on the other diabetic microvascular complications remains unclear. This study was carried out to examine effects of gigantol on high glucose-induced renal cell injury in cultured mouse kidney mesangial cells (MES-13). MES-13 cells were pretreated with gigantol (1, 5, 10 or 20 μmol/L) for 1 h followed by further exposure to high (33.3 mmol/L) glucose for 48 h. Gigantol concentration dependently enhanced cell viability followed by high glucose treatment in MES-13 cells. High glucose induced reactive oxygen species (ROS) generation, malondialdehyde production and glutathione deficiency were recoved in MES-13 cells pretreated with gigantol. High glucose triggered cell apoptosis via the the loss of mitochondrial membrane potential, depletion of adenosine triphosphate, upregulation of caspases 9 and 3, enhancement of cytochrome c release, and subsequent interruption of the Bax/Bcl-2 balance. These detrimental effects were ameliorated by gigantol. High glucose also induced activation of JNK, p38 mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) in MES-13 cells, which were blocked by gigantol. The results suggest that treatment MES-13 cells with gigantol halts high glucose-induced renal dysfunction through the suppression of the ROS/MAPK/NF-κB signaling pathways. Our data are of value to the understanding the mechanism for gigantol, and would benefit the study of drug development or food supplement for diabetes and nephropathy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gigantol has Protective Effects against High Glucose-Evoked Nephrotoxicity in Mouse Glomerulus Mesangial Cells by Suppressing ROS/MAPK/NF-κB Signaling Pathways

et al. 2018

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“…The purification of vandaterosides I and II was monitored by TLC and HPLC analyses. However, to confirm unambiguously their identity, the final fractions obtained were analysed by a targeted HPLC–ESI‐HRMS/MS method previously developed in our group . Results confirmed unequivocally the identity of vandateroside I and II.…”

Section: Resultsmentioning

confidence: 77%

“…The identity of isolated vandaterosides I and II was confirmed by a validated targeted HPLC–ESI‐HRMS/MS method, previously developed by our team . Internal standards were injected separately to obtain their HRMS/MS spectra.…”

Section: Methodsmentioning

confidence: 99%

Purification of vandaterosides from Vanda teres (Orchidaceae) by stepwise gradient centrifugal partition chromatography

Čáková

Urbain

Quemener³

et al. 2015

J of Separation Science

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Vandaterosides are polar glucosyloxybenzyl eucomate derivatives found in Vanda teres (Orchidaceae), which display biological activities that slow the skin ageing process. In order to obtain larger quantities to allow us to go further in the bioassays, the hydroalcoholic extract of aerial parts (leaves and stems) of V. teres were fractionated by centrifugal partition chromatography, combining isocratic, gradient, and dual elution modes. The first fractionation was performed on the extract maintained in the stationary phase as water saturated in butanol, while increasing the polarity of the mobile phase by changing the proportions of ethyl acetate/1-butanol/water, in order to obtain two enriched fractions. Vandateroside I was then purified by isocratic mode with ethyl acetate/ethanol/water (46:14:40), while vandateroside II was obtained by combining isocratic elution with ethyl acetate/isopropanol/water (30:20:50) followed by a multiple dual mode with ethyl acetate/ethanol/water (46:14:40). In this manner, hundreds of milligrams of vandateroside I and II were recovered from 10 g of V. teres extract.

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“…It also enables structural identification of many secondary metabolites present in the complex matrix, in a single run (dereplication of complex matrices). 2–7 …”

Section: Phytochemical Analysismentioning

confidence: 99%

Comparison of analytical techniques for the identification of bioactive compounds from natural products

2016

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Natural product extracts are a rich source of bioactive compounds. As a result, the screening of natural products for the identification of novel biologically active metabolites has been an essential part of several drug discovery programs. It is estimated that more than 70% of all drugs approved from 1981 and 2006, were either derived from or structurally similar to nature based compounds indicating the necessity for the development of a rapid method for the identification of novel compounds from plant extracts. The screening of biological matrices for the identification of novel modulators is nevertheless still challenging. In this review we discuss current techniques in phytochemical analysis and the identification of biologically active components.

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Identification of Phenanthrene Derivatives in Aerides rosea (Orchidaceae) Using the Combined Systems HPLC–ESI–HRMS/MS and HPLC–DAD–MS–SPE–UV–NMR

Cited by 22 publications

References 24 publications

Gigantol has Protective Effects against High Glucose-Evoked Nephrotoxicity in Mouse Glomerulus Mesangial Cells by Suppressing ROS/MAPK/NF-κB Signaling Pathways

Gigantol has Protective Effects against High Glucose-Evoked Nephrotoxicity in Mouse Glomerulus Mesangial Cells by Suppressing ROS/MAPK/NF-κB Signaling Pathways

Purification of vandaterosides from Vanda teres (Orchidaceae) by stepwise gradient centrifugal partition chromatography

Comparison of analytical techniques for the identification of bioactive compounds from natural products

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