Purification of vandaterosides from Vanda teres (Orchidaceae) by stepwise gradient centrifugal partition chromatography

Abstract

Vandaterosides are polar glucosyloxybenzyl eucomate derivatives found in Vanda teres (Orchidaceae), which display biological activities that slow the skin ageing process. In order to obtain larger quantities to allow us to go further in the bioassays, the hydroalcoholic extract of aerial parts (leaves and stems) of V. teres were fractionated by centrifugal partition chromatography, combining isocratic, gradient, and dual elution modes. The first fractionation was performed on the extract maintained in the stationary phase as water saturated in butanol, while increasing the polarity of the mobile phase by changing the proportions of ethyl acetate/1-butanol/water, in order to obtain two enriched fractions. Vandateroside I was then purified by isocratic mode with ethyl acetate/ethanol/water (46:14:40), while vandateroside II was obtained by combining isocratic elution with ethyl acetate/isopropanol/water (30:20:50) followed by a multiple dual mode with ethyl acetate/ethanol/water (46:14:40). In this manner, hundreds of milligrams of vandateroside I and II were recovered from 10 g of V. teres extract.

Genome assembly and chemogenomic profiling of National Flower of Singapore Papilionanthe Miss Joaquim ‘Agnes’ reveals metabolic pathways regulating floral traits

“…Vandaterosides, which are glucosyloxybenzyl eucomate derivatives, were first discovered in Ple. Teres stems 49 . In a previous study, human immortalized keratinocyte cell lines (HaCaT) stimulation with eucomic acid and vandateroside II was shown to upregulate cytochrome c oxidase without enhancing mitochondrial biogenesis 74 .…”

A review on the phytochemical properties of five selected genera of orchids

“…Similarly, Vandaterosides which are eucomate derivatives were isolated from different solvent fraction prepared from Vanda teres extract. 58 In another study conducted using three solvents namely Petroleum ether, ethanol and methanol of plant extraction, it was found that in petroleum ether extract alkaloid, saponin and tannin were present, and in ethanolic extract alkaloid, steroid, saponin and tannin were found to be present whereas in the methanolic extract alkaloid, reducing sugars, flavonoid, steroid, saponin and tannin were present. 59…”