Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea

Abstract

This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.

Differences among Endophytic Fungal Communities Isolated from the Roots ofCephalanthera longibracteataCollected from Different Sites in Korea

“…Twelve species of endophytic fungi, including Tulasnella calospora , which is a mycorrhizal fungus, were isolated from C. longibracteata in Cheongju. T. calospora is the mycorrhizal fungal species the most frequently isolated from terrestrial orchids and has been isolated from several native orchid species in Korea, including Cymbidium goeringii , Neolindleya camtschatica , Oreorchis patens , Spiranthes sinensis , and Cephalanthera falcata [ 24 25 ]; it was first isolated from C. longibracteata .…”

Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea

“…뿐만 아니라 난 균근균은 난의 생존과 분포, 그리고 경쟁관계에도 영향을 주는 것으로 알려져 있다 (Batty et al, 2006;Rasmussen et al, 1993 (Lee, 2011). 대다수의 난 균근균은 무성세대인 Rhizoctonia 속이며 완 전세대형은 Ceratobasidium, Sebacina, Tulasnella 속으로 알려 져 있다 (Sneh et al, 1991 체들을 가지는 것으로 알려져 있다 (Taylor et al, 2003 (Sim et al, 2010;Youm et al, 2011, Youm et al, 2012…”