Differences among Endophytic Fungal Communities Isolated from the Roots ofCephalanthera longibracteataCollected from Different Sites in Korea

Abstract

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

Isolation, characterization, and plant growth-promoting activities of endophytic fungi from a wild orchid Vanda cristata

“…11,14 The in vitro culture of selected samples on different growth medium showed that the diversity of endophytic fungi was found higher in PDA than the Czapek's medium might be the suitable range of pH and nutrients conditions in the PDA medium. 14,15 Fungal diversity and richness of this study was slightly differed than previous studies which might be due to the differences in environmental conditions, the climatic factor of orchid's habitat as well as differences in culture medium and conditions of laboratories among the different researches. Whereas, Fusarium, Alternaria species were dominant in overall isolated endophytic fungi.…”

A comprehensive review on fungal endophytes and its dynamics on Orchidaceae plants: current research, challenges, and future possibilities

“…Most vulnerable orchids have inhabitants of <100 plants and their management is one of the key encounters faced by cultivators [6]. Determining influences disturbing the spatial dispersal and richness of endangered Orchidaceae plants is a chief encounter in existing protection biology [7]. Endophytes have shown a synergetic cooperation of fungus with their Orchidaceae family [8].…”

Diversity of Saprobic Fungi on Magnolia garrettii: Do Collecting Sites and Seasons Affect the Fungal Community?

“…It is also possible that rapid growth of certain fungal species may suppress other slow-growing ones sharing the same niche. SITE SPECIFICITY Lee et al (2017) reported that species diversity and richness of endophytic fungi colonizing the roots of Cephalanthera longibracteata were not significantly different between sites. However, the community structure of the endophytic fungi did significantly differ between sites, suggesting that site characteristics affected the community composition of those endophytic fungi.…”