Identification of Novel Imprinted Genes in a Genome-Wide Screen for Maternal Methylation

Smith, Rachel J.; Dean, Wendy; Konfortova, Galia; Kelsey, Gavin

doi:10.1101/gr.781503

Cited by 166 publications

(161 citation statements)

References 48 publications

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“…It was originally thought that SLC38A4/SNAT4 was a liver-specific isoform (Hatanaka et al, 2001). However, we have shown this isoform is present in human placenta (Desforges et al, 2006) and expression has also been confirmed in rat (Novak et al, 2006a) and mouse placenta (Mizuno et al, 2002;Smith et al, 2003).…”

Section: Molecular Characterisation and Expression In Human Placentamentioning

confidence: 99%

Placental nutrient supply and fetal growth

Desforges¹,

Sibley²

2010

Int. J. Dev. Biol.

145

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This review considers mechanisms by which transfer across the placenta takes place and how the capacity of the placenta to supply nutrients relates to fetal growth and vice versa. Blood flow through both uterine and umbilical circulations of the placenta, the structural properties of the placental exchange barrier and its related diffusional permeability, and the expression and activity of a wide range of transporter proteins in the syncytiotrophoblast, the transporting epithelium of the placenta, all need to be taken into account in considering placental supply capacity. We discuss the evidence that each of these factors affects, and is affected by, fetal growth rate and consider the regulatory mechanisms involved, with a particular focus on data that has emerged from study of the system A amino acid transporter. We consider that future work will build on the considerable foundation of knowledge regarding placental transfer mechanisms, as well as the other aspects of placental structure and function, to develop new diagnostic and therapeutic strategies for pregnancy complications, such as fetal growth restriction or overgrowth.

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Section: Molecular Characterisation and Expression In Human Placentamentioning

confidence: 99%

Placental nutrient supply and fetal growth

Desforges¹,

Sibley²

2010

Int. J. Dev. Biol.

145

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“…The function of NAP1L5 is not known, but its protein shows homology to nucleosome assembly proteins (NAPs) which are involved in cell cycle regulation (Loyola and Almouzni, 2004). The NAP1L5 is paternally expressed in many fetal tissues in human, mouse and cattle (Smith et al, 2003;Zaitoun and Khatib, 2006;Wood et al, 2007). The PPP1R9A encodes neurabin I which is a regulatory subunit of protein phosphatase I, and controls actin cytoskeleton reorganization (Oliver et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, mRNA expression and imprinting status of PEG3, NAP1L5 and PPP1R9A genes in pig

Zhang

Deng

et al. 2011

Genes Genet. Syst.

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Imprinted genes are expressed monoallelically depending on their parental origin, and play important roles in the regulation of fetal growth, development, and postnatal behavior. Most genes known to be imprinted have been identified and studied in the human and the mouse. However, there are only a small number of reported imprinted genes in pigs. Therefore, identification and characterization of more imprinted genes in pigs is useful for comparative analysis of genomic imprinting across species. In this study, we cloned the porcine PEG3, NAP1L5 and PPP1R9A genes. The imprinting status of these genes was determined using sequencing directly and single nucleotide polymorphisms (SNPs) identified in individuals from reciprocal cross of Meishan and Large White pigs. Imprinting analysis was carried out in 13 different tissues (skeletal muscle, fat, pituitary gland, heart, lung, liver, kidney, spleen, stomach, small intestine, uterus, ovary and testis) from twelve 2-month-old piglets. Imprinting analysis showed that PEG3 and NAP1L5 were exclusively expressed from the paternal allele whereas PPP1R9A was biallelically expressed in all tissues tested where the genes were expressed. The study is of interest to understand the conservation of genomic imprinting among mammals at the 3 loci.

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“…Genomic imprinting is a parent-of-origin-dependent epigenetic mechanism, in which a subset of autosomal genes is expressed from only one allele [15]. Imprinted genes have important roles in the regulation of foetal growth, development, function of the placenta and postnatal behaviour, in mammals in particular [14].…”

Section: Introductionmentioning

confidence: 99%

NNATandDIRAS3genes are paternally expressed in pigs

et al. 2007

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-Although expression and epigenetic differences of imprinted genes have been extensively characterised in man and the mouse, little is known on livestock species. In this study, the polymorphism-based approach was used to detect the imprinting status of NNAT and DIRAS3 genes in five heterozygous pigs (based on SNP) of Large White and Meishan F 1 hybrids. The results show that both genes were paternally expressed in all the tested tissues (heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary and pituitary). In addition, the NNAT gene had two transcripts in all tested tissues, which is consistent with its counterpart in man and cattle.pig / NNAT / DIRAS3 / imprinting / paternally expressed

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Identification of Novel Imprinted Genes in a Genome-Wide Screen for Maternal Methylation

Cited by 166 publications

References 48 publications

Placental nutrient supply and fetal growth

Placental nutrient supply and fetal growth

Molecular cloning, mRNA expression and imprinting status of PEG3, NAP1L5 and PPP1R9A genes in pig

NNATandDIRAS3genes are paternally expressed in pigs

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