<i>NNAT</i>and<i>DIRAS3</i>genes are paternally expressed in pigs

Cheng, Hongyang; Zhang, Fengwei; Deng, Chang-Yan; Jiang, Caode; Xiong, Yan; Li, Fenge; Lei, Minggang

doi:10.1051/gse:2007024

Cited by 2 publications

(2 citation statements)

References 20 publications

(13 reference statements)

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“…In pigs, a few imprinted genes have been found to be expressed monoallelically in somatic tissues [8], [9]. Recently, we and others have confirmed the methylation patterns of IGF2/H19 DMRs in preimplantation embryos and in primordial germ cells, respectively [10], [11].…”

Section: Introductionsupporting

confidence: 52%

Analysis of Imprinted Gene Expression in Normal Fertilized and Uniparental Preimplantation Porcine Embryos

Park

Mulligan

et al. 2011

PLoS ONE

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In the present study quantitative real-time PCR was used to determine the expression status of eight imprinted genes (GRB10, H19, IGF2R, XIST, IGF2, NNAT, PEG1 and PEG10) during preimplantation development, in normal fertilized and uniparental porcine embryos. The results demonstrated that, in all observed embryo samples, a non imprinted gene expression pattern up to the 16-cell stage of development was common for most genes. This was true for all classes of embryo, regardless of parental-origins and the direction of imprint. However, several differentially expressed genes (H19, IGF2, XIST and PEG10) were detected amongst the classes at the blastocyst stage of development. Most interestingly and despite the fact that maternally and paternally expressed genes should not be expressed in androgenones and parthenogenones, respectively, both uniparental embryos expressed these genes when tested for in this study. In order to account for this phenomenon, we compared the expression patterns of eight imprinted genes along with the methylation status of the IGF2/H19 DMR3 in haploid and diploid parthenogenetic embryos. Our findings revealed that IGF2, NNAT and PEG10 were silenced in haploid but not diploid parthenogenetic blastocysts and differential methylation of the IGF2/H19 DMR3 was consistently observed between haploid and diploid parthenogenetic blastocysts. These results appear to suggest that there exists a process to adjust the expression status of imprinted genes in diploid parthenogenetic embryos and that this phenomenon may be associated with altered methylation at an imprinting control region. In addition we believe that imprinted expression occurs in at least four genes, namely H19, IGF2, XIST and PEG10 in porcine blastocyst stage embryos.

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Section: Introductionsupporting

confidence: 52%

Analysis of Imprinted Gene Expression in Normal Fertilized and Uniparental Preimplantation Porcine Embryos

Park

Mulligan

et al. 2011

PLoS ONE

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“…In the pig, a study showed that NNAT was imprinted in 11 tissues, including heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus, ovary, and pituitary gland [12], but in pig placenta, the expression status inherited from parents was still uncovered to our knowledge.…”

Section: Introductionmentioning

confidence: 98%

Molecular Characterization of the Neuronatin Gene in the Porcine Placenta

Zhou

et al. 2012

PLoS ONE

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Imprinted genes play important roles in placental and embryonic development. Neuronatin (NNAT), first identified as an imprinted gene in human and mouse brains, played important roles in neuronal differentiation in the brain and in glucose-mediated insulin secretion in pancreatic β cells. In the pig, NNAT was reported to be imprinted in eleven tissues. Our previous microarray hybridization study showed that NNAT was differentially expressed in Yorkshire and Meishan pig placentas, but the imprinting status and function of NNAT in the placenta have not been investigated. We demonstrated for the first time that NNAT was monoallelically expressed in the placenta. Immunochemistry analysis showed that NNAT was located in the uterine luminal and glandular epithelium in placentas. We also confirmed the differential expression of NNAT in Meishan and Yorkshire pig placentas by qPCR. Using IPA software and the published literature, we created a model network of the possible relationships between NNAT and glucose transporter genes. A dual luciferase reporter assay demonstrated that the crucial promoter region of NNAT contained a CANNTG sequence in the +210 to +215 positions, which corresponded to the E-box. Our findings demonstrated important roles of NNAT in placenta function.

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NNATandDIRAS3genes are paternally expressed in pigs

Cited by 2 publications

References 20 publications

Analysis of Imprinted Gene Expression in Normal Fertilized and Uniparental Preimplantation Porcine Embryos

Analysis of Imprinted Gene Expression in Normal Fertilized and Uniparental Preimplantation Porcine Embryos

Molecular Characterization of the Neuronatin Gene in the Porcine Placenta

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