Identification of key pathways and genes influencing prognosis in bladder urothelial carcinoma

Ning, Xin; Deng, Yaoliang

doi:10.2147/ott.s131386

Cited by 23 publications

(20 citation statements)

References 76 publications

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“…On the other hand, mTORC2 also increases cellular proliferation and survival through the regulation of protein kinases, including Akt 32 , placing mTOR on both sides of the Akt signaling hub. Previous studies have revealed that 6/18 (decorin, biglycan, asporin, fibromodulin, PRELP, osteoglycin) of SLRP members function via regulating PI3K/Akt/mTOR pathway 24 , 33 - 37 , which suggests that the potential relationship between SLRP and PI3K/Akt/mTOR axis. In the present, PODNL1 knockdown inactivated Akt/mTOR signaling pathway and overexpression showed the opposite results.…”

Section: Discussionmentioning

confidence: 99%

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

Geng

Zuo

et al. 2020

J. Cancer

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Background and Aims: Emerging studies have determined that the small leucine-rich proteoglycan (SLRP) family can aggravate tumor progression. However, the biological function of podocan-like protein 1 (PODNL1), a novel member of the SLRP family, has not been investigated. Therefore, our study focused on the function and regulatory mechanism of PODNL1 in glioma. Methods: Both the Gene Expression Profiling Interactive Analysis (GEPIA) and the Chinese Glioma Genome Atlas (CGGA) database were used to analyze the expression level and survival risk of PODNL1 in glioma. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were applied to detect the mRNA and protein expression, respectively. Celltiter-Glo and colony formation assays were used to evaluate cell proliferation. Migration capacity was measured by Transwell and wound healing assays. Flow cytometry was utilized to assess the apoptotic rate. Results: The expression of PODNL1 predicted the poor prognosis in glioma patients. Silencing of PODNL1 inhibited cell proliferation, migration, and induced epithelial-like phenotype. In addition, knockdown of PODNL1 also induced cell apoptosis. Moreover, the cell growth and migration inhibited by PODNL1 knockdown could be partially rescued with Akt activator. Conversely, PODNL1 overexpression promoted cell growth and migration, which were suppressed by Akt inhibitor. Conclusions: PODNL1, a promising predictive indicator of poor prognosis, resulted in greater proliferation, migration and epithelial-mesenchymal transition (EMT) process. Moreover, PODNL1 promoted aggressive glioma behavior by activating Akt/mTOR pathway, providing a novel therapeutic target for glioma.

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Section: Discussionmentioning

confidence: 99%

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

Geng

Zuo

et al. 2020

J. Cancer

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“…For example, the CBFB/MYH11 fusion gene has been implicated in the onset of acute myeloid leukemia (35,36). In addition, MYH11 gene expression levels have been associated with the prognostic outcome of the bladder urothelial carcinoma, notably in patients with advanced tumors (37). Seitz et al (38) demonstrated that MYH11 gene expression levels were downregulated in breast tumor and metastasis, using microarray and correlation analyses.…”

Section: Discussionmentioning

confidence: 99%

Clinical and prognostic significance of MYH11 in lung cancer

et al. 2020

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Myosin heavy chain 11 (MYH11), encoded by the MYH11 gene, is a protein that participates in muscle contraction through the hydrolysis of adenosine triphosphate. Although previous studies have demonstrated that MYH11 gene expression levels are downregulated in several types of cancer, its expression levels have rarely been investigated in lung cancer. The present study aimed to explore the clinical significance and prognostic value of MYH11 expression levels in lung cancer and to further study the underlying molecular mechanisms of the function of this gene. The Oncomine database showed that the MYH11 expression levels were decreased in lung cancer compared with those noted in the normal lung tissue (P<0.05). Kaplan-Meier plotter results revealed that the decreased MYH11 expression levels were correlated with poor prognosis in lung cancer patients. Among the lung cancer cases with gene alteration of MYH11, mutation was the most common of all alteration types. Coexpedia and Metascape analyses revealed that the target genes were primarily enriched in 'muscle contraction', 'contractile fiber part', 'actin cytoskeleton' and the 'adherens junction'. These results indicated that MYH11 is a potential novel drug target and prognostic indicator of lung cancer.

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“…The MEK–ERK pathway plays an important role in cell proliferation and participates in the genesis of many epithelial cancers. The MEK–ERK pathway participates in several cellular processes such as proliferation, differentiation, and motility and plays an important role in BC prognosis 32,33. This pathway is often upregulated in cancer tissues and plays an vital role in anticancer therapy 34.…”

Section: Discussionmentioning

confidence: 99%

<p>KIF15 promotes bladder cancer proliferation via the MEK–ERK signaling pathway</p>

Zhao

et al. 2019

CMAR

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Background Bladder cancer (BC) is the most common cancer of the urinary tract and invariably predicts a poor prognosis. In this study, we found a reliable gene signature and potential biomarker for predicting clinical prognosis. Methods The gene expression profiles were obtained from the GEO database. By performing GEO2R analysis, numerous differentially expressed genes (DEGs) were found. Three different microarray datasets were integrated in order to more precisely identify up-expression genes. Functional analysis revealed that these genes were mainly involved in cell cycle, DNA replication and metabolic pathways. Results Based on protein-protein interactome (PPI) networks that were identified in the current study and previous studies, we focused on KIF15 for further study. The results showed that KIF15 promotes BC cell proliferation via the MEK -ERK pathway, and Kaplan‐Meier survival analysis revealed that KIF15 expression was an independent prognostic risk factor in BC patients. Conclusion KIF15 may represent a promising prognostic biomarker and a potential therapeutic option for BC.

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Identification of key pathways and genes influencing prognosis in bladder urothelial carcinoma

Cited by 23 publications

References 76 publications

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

Clinical and prognostic significance of MYH11 in lung cancer

<p>KIF15 promotes bladder cancer proliferation via the MEK–ERK signaling pathway</p>

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Identification of key pathways and genes influencing prognosis in bladder urothelial carcinoma

Cited by 23 publications

References 76 publications

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

PODNL1 promotes cell proliferation and migration in glioma via regulating Akt/mTOR pathway

Clinical and prognostic significance of MYH11 in lung cancer

<p>KIF15 promotes bladder cancer proliferation via the MEK&ndash;ERK signaling pathway</p>

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<p>KIF15 promotes bladder cancer proliferation via the MEK–ERK signaling pathway</p>