&lt;p&gt;KIF15 promotes bladder cancer proliferation via the MEK&amp;ndash;ERK signaling pathway&lt;/p&gt;

Zhao, Hongda; Bo, Qiyu; Wu, Zonglong; Liu, Qinggang; Li, Yan; Zhang, Ning; Guo, Hu; Shi, Benkang

doi:10.2147/cmar.s191681

Cited by 22 publications

(22 citation statements)

References 35 publications

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“…Although the structure and molecular functions of KIF15 have been studied for approximately 10 years, the role of KIF15 in the tumorigenesis and progression of OC has not yet been illuminated. A previous study had reported that KIF15 promoted the proliferation of cancer cells in pancreatic cancer [16], bladder cancer [17], breast cancer [18], and osteosarcoma [19]. It indicated that KIF15 was a proliferation-related biomarker in multiple malignancies, which we had veri ed on ovarian cancer in this study.…”

Section: Discussionsupporting

confidence: 58%

Bioinformatic and Experimental Analysis Reveals Knockdown of KIF15 Promotes Cell Apoptosis via Activating Crosstalk of Multi-Pathways in Ovarian Cancer

Sun¹,

Chen²,

Liao³

et al. 2020

Preprint

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Background: Ovarian cancer (OC) is the most lethal malignancy of females worldwide. Unlimited proliferation is a fundamental feature of OC cells. The genes associated with cell proliferation are potential histopathological biomarkers and targets of anti-tumor therapeutic strategies. In the present study, we aimed to identify proliferation-associated biomarkers with potential prognostic, diagnostic, and therapeutic value and reveal the underlying molecular mechanism of the candidate gene involved in OC by a combination of bioinformatic and experimental methods.Results: KIF15 was upregulated in early-stage OC tissues and could predict poor prognosis of patients of Stage I and II. The knockdown of KIF15 significantly inhibited cell proliferation, tumor formation, and growth as well as promoted apoptosis of OC cells. A combination of experimental and bioinformatic analyses revealed KIF15 knockdown promoted cell apoptosis via activating crosstalk of multi-pathways in OC.Conclusion: In this study, KIF15, an early-stage prognostic gene, was identified as a potential histopathological biomarker and therapeutic target of OC.

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Section: Discussionsupporting

confidence: 58%

Bioinformatic and Experimental Analysis Reveals Knockdown of KIF15 Promotes Cell Apoptosis via Activating Crosstalk of Multi-Pathways in Ovarian Cancer

Sun¹,

Chen²,

Liao³

et al. 2020

Preprint

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“…Previous studies have proposed that KIFs play important roles in different diseases even various of cancers. It can lead to congenital thrombocytopenia, breast cancer, oral cancer and bladder cancer [8,[23][24][25][26]. Recently, KIF15 has been reported to be highly expressed in gastrointestinal (GI) track cancers such as pancreatic cancer and hepatocellular cancer [16,27].…”

Section: Discussionmentioning

confidence: 99%

KIF15 facilitates gastric cancer via enhancing proliferation, inhibiting apoptosis, and predict poor prognosis

Ding

et al. 2020

Cancer Cell Int

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Background: Kinesin superfamily proteins (KIFs) can transport membranous organelles and protein complexes in an ATP-dependent manner. Kinesin family member 15 (KIF15) is overexpressed in various cancers. However, the function of KIF15 in gastric cancer (GC) is still unclear. Methods: GC patients' data from The Cancer Genome Atlas (TCGA) were analyzed by bioinformatics methods. The expression of KIF15 was examined in GC and paracarcinoma tissues from 41 patients to verify the analysis results. The relationship between KIF15 expression and clinical characteristics were also observed by bioinformatics methods. Kaplan-Meier survival analysis of 122 GC patients in our hospital was performed to explore the relationship between KIF15 expression levels and GC patients' prognosis. KIF15 was downregulated in GC cell lines AGS and SGC-7901 by transfecting a lentivirus-mediated shRNA plasmid targeting KIF15. In vitro, GC cell proliferation and apoptosis were detected by MTT assay, colony formation assay, and Annexin V-APC staining. In vivo, xenograft experiments were used to verify the in vitro results. Furthermore, Human Apoptosis Antibody Array kit was used to screen possible targets of KIF15 in GC cell lines. Results: The bioinformatics results showed that KIF15 expression levels were higher in GC tissues than in normal tissues. IHC showed same results. High expression of KIF15 was statistical correlated with high age and early histologic stage. Kaplan-Meier curves indicated that high KIF15 expression predict poor prognosis in patients with GC. MTT assay and colony formation assay showed that KIF15 promote GC cell proliferation. Annexin V-APC staining found that KIF15 can inhibit GC cell apoptosis. Xenograft experiments reveal that downregulating KIF15 can inhibit GC tumor growth and promote GC apoptosis. Through detection of 43 anti-apoptotic proteins by the Human Apoptosis Antibody Array kit, it was confirmed that knocking down KIF15 can reduce seven anti-apoptotic proteins expression. Conclusions: Taken together, our study revealed a critical role for KIF15 to inhibit GC cell apoptosis and promote GC cell proliferation. KIF15 may decrease anti-apoptotic proteins expression by regulating apoptosis pathways. High expression of KIF15 predicts a poor prognosis in patients with GC. KIF15 might be a novel prognostic biomarker and a therapeutic target for GC.

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“…KIF15 mediated pancreatic cancer proliferation through regulating the cell cycle and apoptosis [22]. KIF15 may be a potential prognostic biomarker in bladder cancer [23]. RNF113A expression is correlated with a poor prognosis of esophageal squamous cell carcinoma [24].…”

Section: Discussionmentioning

confidence: 99%

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer

Xiao

2020

Bioengineered

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Advanced colorectal cancer (CRC) is a significant cause of cancer mortality, with a poor prognosis. Here, we identified a novel prognostic signature for predicting survival of advanced CRC. Advanced CRC data were used (training set: n = 267 and validation set: n = 264). The survival analyses were investigated. The functional analysis of the prognostic signature was examined. In this study, our 15-gene signature was established and was an independent prognostic factor of advanced CRC. Stratification analyses also showed that this signature was still powerful for survival prediction in each stratum of age, gender, stage, and metastasis status. In mechanism, our signature involved in DNA replication, DNA damage, and cell cycle. Therefore, our findings suggested that this 15-gene signature has prognostic and predictive value in advanced CRC, which could be further used in personalized therapy for advanced CRC. ARTICLE HISTORY

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<p>KIF15 promotes bladder cancer proliferation via the MEK–ERK signaling pathway</p>

Cited by 22 publications

References 35 publications

Bioinformatic and Experimental Analysis Reveals Knockdown of KIF15 Promotes Cell Apoptosis via Activating Crosstalk of Multi-Pathways in Ovarian Cancer

Bioinformatic and Experimental Analysis Reveals Knockdown of KIF15 Promotes Cell Apoptosis via Activating Crosstalk of Multi-Pathways in Ovarian Cancer

KIF15 facilitates gastric cancer via enhancing proliferation, inhibiting apoptosis, and predict poor prognosis

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer

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