Identification of Isoflavones in the Roots of<i>Pueraria lobata</i>

Rong, Haojing; Stevens, Jan F.; Deinzer, Max L.; Cooman, Luc De; Keukeleire, Denis De

doi:10.1055/s-2006-957534

Cited by 94 publications

(59 citation statements)

References 7 publications

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“…This traditional Chinese medicine has been used for the treatment of various diseases and to promote bone healing for millenia and recent data suggest beneficial activity (16)(17)(18)(19)(20). The active ingredients have been identified as the isoflavones: puerarin, daidzin and daidzein, which have estrogenic activity (21)(22)(23)(24). Puerariae radix has shown positive therapeutic effects in animal studies (25)(26)(27) and in cell culture studies, Puerariae radix was shown to induce osteogenic activity (20,(28)(29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

et al. 2010

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“…The observation that Gen has a relatively strong uterotrophic effect but a minor obesity preventive effect is of interest and points to some tissue specificity of this isoflavone. Puer with a molecular weight (MW) of 416 is the glycosylated form of Daid (MW 254) and is deglycosylated by the intestinal flora (Rong et al, 1998). Hence, the active principle is Daid and the mean daily uptake of 44 mg Puer would amount to 27 mg Daid after deglycosylation.…”

Section: Discussionmentioning

confidence: 99%

“…The present study shows that from the 3 phytoestrogens tested, Puer had the strongest estrogenic activity in urinary bladder/urethral system. By investigating isoflavones contained in Pueraria lobata (Kudzu) with high-performance liquid chromatography coupled to photodiode array and to mass spectroscopy, (Rong et al, 1998) showed that Puer is the glycosylated form of Daid and they concluded that estrogenic activity occurs only after deglycosylation by gut bacteria which takes time. It may therefore be assumed that Puer has a depot effect.…”

Section: Discussionmentioning

confidence: 99%

Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats

Thielemann

Wuttke

et al. 2010

Experimental Gerontology

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Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats. Experimental Gerontology, Elsevier, 2010, 45 (2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractWhether urinary incontinence in the postmenopause can be prevented or delayed by estrogens is currently controversially debated. Ovariectomized (ovx) rats have been successfully used as models in the past years but plant derived substances with estrogenic effects in the lower urinary tract have not been studied so far. Therefore we compared the effects of a 3 months lasting oral administration of estradiol 17ß (E2) with those of the phytoestrogens equol, genistein and puerarin. They were then anaesthetized and catheterized with a biluminal catheter having one outlet in the bladder and another in the urethra at the level of the urethral sphincter. Urethral and bladder pressure were recorded during a 240 sec period of retrograde bladder filling (2 x 0.5 ml within 30 s with 1 min filling intermission). Bladder and urethra pressures were highest in the E2>puerarin>equol>genistein treated animals. Phytoestrogen and E2 treatment resulted in consistently higher urethral than the bladder pressures during the filling period and in the filled status whereas bladder often exceeded urethral pressures in ovx controls. In summary, we demonstrate significant improvement of urethral closure mechanism under E2 and phytoestrogen administration that can be assumed to be beneficial for prevention or therapy of postmenopausal urge incontinence.

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“…Their structures were elucidated using comprehensive spectroscopic methods including 1D and 2D NMR, MS, UV and IR, combined with a variety of chemical methods, and by comparison their data with those reported previously in the literature. The isolated compounds were involved in ten types of chemical structures, including fourteen flavonoid glycosides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14), ten flavonoids (15)(16)(17)(18)(19)(20)(21)(22)(23)(24), four lignans (25)(26)(27)(28), seven phenolic acid compounds (29-34, 42), one steroid (35), one diterpene (36), one monoterpene (37), one phenolic glycoside (38), three iridoid glycosides (39)(40)(41) and one steroid saponin (43) ( Table 1). Among the isolated compounds, about of 50% of them were flavonoid glycosides and flavonoids, and it could be concluded that flavonoid glycosides, flavonoids, phenolic compounds and lignans were the major structure types in the EtOAc and n-BuOH fractions of SCP.…”

Section: Chemical Constituents Of the Etoac And N-buoh Fractions Frommentioning

confidence: 99%

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

Liu¹,

Wen²,

Sun³

et al. 2016

Afr. J. Trad. Compl. Alt. Med.

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Background: Seaweed Complex Preparation (SCP) is a clinical traditional Chinese medicine preparation composed of seven traditional Chinese herbs, and has been used for treatment of lung cancer, liver cancer and digestive cancer. Materials and Methods: In this study, the potential bioactive constituents of SCP were isolated and identified by chromatographic and spectroscopic methods. The immunomodulatory and DPPH radical scavenging activities of the constituents were also evaluated in vitro. Results: Forty-three compounds were isolated from the EtOAc and n-BuOH fractions of the decocted extracts. More than 10 structural types of chemical constituents were found, including flavonoid glycosides, flavonoids, phenolic acid compounds, lignans, iridoid glycosides, and polysaccharides mainly composed of galactose and glucose. Flavonoid glycosides exhibited potential immunomodulatory by promoting splenic lymphocyte proliferation and stimulating the secretion of cytokines IL-2 and IFN-γ, as well as free radical (DPPH) scavenging activities. Caffeic acid derivatives showed remarkable free radical (DPPH) scavenging activity, while polysaccharides also displayed immunological enhancement effects. Conclusion: Four molecular communities, flavonoid glycosides, flavonoids, phenolic compounds and polysaccharides, might be the potential bioactive components of SCP. Synergism of these four molecular communities in SCP may endue the anticancer properties and reflect the feature of multi-components and multi-targets of traditional Chinese medicine formula.

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Identification of Isoflavones in the Roots ofPueraria lobata

Cited by 94 publications

References 7 publications

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats

Screening and identification of potential bioactive constituents in a new herbal prescription seaweed complex preparation

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