Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Kantawong, Fahsai; Burgess, Karl; Jayawardena, K. G. I.; Hart, Andrew; Riehle, Mathis O.; Oreffo, Richard O.C.; Dalby, Matthew J.; Burchmore, Richard

doi:10.1016/j.actbio.2010.02.038

Cited by 21 publications

(20 citation statements)

References 64 publications

(46 reference statements)

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“…The cytoplasmic domains of integrins bind to the cytoskeleton through adapter proteins like vinculin, α-actinin, and phosphorylated-focal adhesion kinase (p-FAK). These protein complexes form focal adhesions, i.e., sub-cellular sites that receive and transduce physiochemical signals from a substrate [57], [58] to influence cell morphology [59], [60], adhesion [61], proliferation [62], motility [63], differentiation [64], and cell fate [55]. The cytoplasmic domains of β-integrin also interact with talin, filamin, tesin, and other focal adhesion proteins to stabilize or destabilize focal adhesions [55], [65], [66], [67], leading to the remodeling of microfilament and microtubule networks, which in turn affects gene transfer and expression.…”

Section: Discussionmentioning

confidence: 99%

A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells

et al. 2012

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Human induced pluripotent stem cells have the potential to become an unlimited cell source for cell replacement therapy. The realization of this potential, however, depends on the availability of culture methods that are robust, scalable, and use chemically defined materials. Despite significant advances in hiPSC technologies, the expansion of hiPSCs relies upon the use of animal-derived extracellular matrix extracts, such as Matrigel, which raises safety concerns over the use of these products. In this work, we investigated the feasibility of expanding and differentiating hiPSCs on a chemically defined, xeno-free synthetic peptide substrate, i.e. Corning Synthemax® Surface. We demonstrated that the Synthemax Surface supports the attachment, spreading, and proliferation of hiPSCs, as well as hiPSCs’ lineage-specific differentiation. hiPSCs colonies grown on Synthemax Surfaces exhibit less spread and more compact morphology compared to cells grown on Matrigel™. The cytoskeleton characterization of hiPSCs grown on the Synthemax Surface revealed formation of denser actin filaments in the cell-cell interface. The down-regulation of vinculin and up-regulation of zyxin expression were also observed in hiPSCs grown on the Synthemax Surface. Further examination of cell-ECM interaction revealed that hiPSCs grown on the Synthemax Surface primarily utilize αvβ5 integrins to mediate attachment to the substrate, whereas multiple integrins are involved in cell attachment to Matrigel. Finally, hiPSCs can be maintained undifferentiated on the Synthemax Surface for more than ten passages. These studies provide a novel approach for expansion of hiPSCs using synthetic peptide engineered surface as a substrate to avoid a potential risk of contamination and lot-to-lot variability with animal derived materials.

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Section: Discussionmentioning

confidence: 99%

A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells

et al. 2012

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“…Huh et al reported that puerariae radix potently induced osteoblastic differentiation markers such as ALP, VEGF, OCN, OPN, Col I, and mineralization in SaOS‐2 cells13 15. A more recent study demonstrated that puerariae powder combined with microgrooved topography stimulated STRO‐1‐positive stem cells differentiate along an osteoblastic phenotype 26…”

Section: Discussionmentioning

confidence: 99%

Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study

Yang

Zhang

et al. 2012

J Biomedical Materials Res

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Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin-loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3-E1). Puerarin was prepared onto titanium surfaces with varying concentration (10(-9) M, 10(-8) M, 10(-7) M, and 10(-6) M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field-emission scanning electron microscope (FSEM), X-ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High-performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10(-8) M group was 10.22 ± 0.32 ng/cm(2) . Puerarin had an increased effect on MC3T3-E1 ALP activities. Significant differences were found in 10(-8) and 10(-7) M groups on day 4, 10(-8) , 10(-7) , and 10(-6) M groups on day 7, and 10(-8) on day 14. In Type I collagen synthesis assay, 10(-9) and 10(-8) M on day 7, 10(-8) on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10(-8) M and 10(-7) M, maximal at 10(-8) M). These results indicate puerarin-loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration.

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“…From previous work, we know that certain microgrooves induce fibroblast formation from skeletal stem cell enriched (STRO-1) populations [55] and that NSQ50 topography results in osteoblastic, bone differentiation [15]. In this study, increased Runx2 staining is seen on the NSQ50 nanopits compared to the elongated, bipolar ( in vivo fibroblast-like) cells on the microgrooves and the polarized, ( in vitro fibroblast-like) cells on planar control (Figures 3 and 6).…”

Section: Discussionmentioning

confidence: 99%

Protein Expression of STRO-1 Cells in Response to Different Topographic Features

Kantawong¹,

Robertson²,

Gadegaard³

et al. 2011

Journal of Tissue Engineering

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Human skeletal stem cells (STRO-1 positive) display distinct responses to different topographical features. On a flat surface, skeletal cells spread, and in vitro, they typically display a polarized, fibroblast-like morphology. However, on microgrooved surfaces, these cells prefer to stretch along the grooves forming a similar morphology to in vivo, bipolarized fibroblasts. In contrast, on nanopits, these cells display a polygonal and osteoblastic phenotype. We have examined mechanotransduction events of STRO-1 positive in response to fibroblastic, microgrooved and osteogenic, controlled disorder nanopit, topographies using proteomics after 3 days in culture. Protein expression profiles were analyzed by difference gel electrophoresis to identify proteins that showed modulation of expression in response to different topographic features to assess early decision events in these cells on these discrete topographies. After only 72 hours in culture, STRO-1 positive displayed differential regulations of families of proteins involved in cell migration and proliferation. The current study indicated that osteogenic decision specific events had already occurred. Runx2 was localized in nuclei of the skeletal stem cells on the osteogenic nanopits; however, few signaling pathway changes were observed. This study demonstrated that micro- and nanotopographies activated skeletal stem cells at different times and with distinct mechanotransduction profiles.

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Effects of a surface topography composite with puerariae radix on human STRO-1-positive stem cells

Cited by 21 publications

References 64 publications

A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells

A Synthetic, Xeno-Free Peptide Surface for Expansion and Directed Differentiation of Human Induced Pluripotent Stem Cells

Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study

Protein Expression of STRO-1 Cells in Response to Different Topographic Features

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