Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study

Abstract

Recent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin-loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3-E1). Puerarin was prepared onto titanium surfaces with varying concentration (10(-9) M, 10(-8) M, 10(-7) M, and 10(-6) M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field-emission scanning electron microscope (FSEM), X-ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High-performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10(-8) M group was 10.22 ± 0.32 ng/cm(2) . Puerarin had an increased effect on MC3T3-E1 ALP activities. Significant differences were found in 10(-8) and 10(-7) M groups on day 4, 10(-8) , 10(-7) , and 10(-6) M groups on day 7, and 10(-8) on day 14. In Type I collagen synthesis assay, 10(-9) and 10(-8) M on day 7, 10(-8) on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10(-8) M and 10(-7) M, maximal at 10(-8) M). These results indicate puerarin-loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration.

Puerarin enhances proliferation and osteoblastic differentiation of human bone marrow stromal cells via a nitric oxide/cyclic guanosine monophosphate signaling pathway

“…In the present study, NO production and the anabolic effect of puerarin on hBMSCs were simultaneously blocked by ICI182780, an estrogen receptor (ER) antagonist, suggesting that puerarin exerts its function through ERs and that the NOS-NO pathway may include downstream effectors of the ER. These findings are consistent with other studies showing the stimulatory effects of puerarin on the osteoblastic cell line MC3T3-E1, which were also blocked by tamoxifen, another anti-estrogen reagent (38).…”

Promotion of bone regeneration on titanium implants through a chemical treatment process using calcium phosphate slurry: Microscopic analysis, cellular response, and animal experiment

“…34 Yang et al reported that biomimetic calcium phosphate coating of titanium promoted accelerated ALP activity at 4, 7, and 14 days and OC production at 14 days. 35 These studies support the results of the present study, which indicated that the chemical properties of HAp itself accelerated the differentiation of bone marrow stromal cells (BMSCs) into osteoblasts. Osteoblasts can migrate and attach to HAp rapidly, supporting marrow stromal cell differentiation into osteoblasts.…”

“…Zhou et al reported that three‐dimensional (3D) Sr 1 ‐HAp nanorods might provide an excellent environment that promotes messenger ribonucleic acid (mRNA) of Runx2, ALP activity, and expression of OP and OC at 7 and 14 days . Yang et al reported that biomimetic calcium phosphate coating of titanium promoted accelerated ALP activity at 4, 7, and 14 days and OC production at 14 days …”

Puerarin: A Review of Pharmacological Effects

“…Puerarin‐loaded titanium surfaces elevated pre‐osteoblasts differentiation in MC3T3‐E1 cells (Yang et al ., ). Puerarin could increase the amount of calcium nodules in umbilical cord mesenchymal stem cells (MSCs), accelerate differentiation of MSCs into osteoblasts, and promote MSC proliferation (Cai et al ., ).…”