Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats

Abstract

Comparison of urodynamic effects of phytoestrogens equol, puerarin and genistein with these of estradiol 17β in ovariectomized rats. Experimental Gerontology, Elsevier, 2010, 45 (2) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT AbstractWhether urinary incontinence in the postmenopause can be prevented or delayed by estrogens is currently controversially debated. Ovariectomized (ovx) rats have been successfully used as models in the past years but plant derived substances with estrogenic effects in the lower urinary tract have not been studied so far. Therefore we compared the effects of a 3 months lasting oral administration of estradiol 17ß (E2) with those of the phytoestrogens equol, genistein and puerarin. They were then anaesthetized and catheterized with a biluminal catheter having one outlet in the bladder and another in the urethra at the level of the urethral sphincter. Urethral and bladder pressure were recorded during a 240 sec period of retrograde bladder filling (2 x 0.5 ml within 30 s with 1 min filling intermission). Bladder and urethra pressures were highest in the E2>puerarin>equol>genistein treated animals. Phytoestrogen and E2 treatment resulted in consistently higher urethral than the bladder pressures during the filling period and in the filled status whereas bladder often exceeded urethral pressures in ovx controls. In summary, we demonstrate significant improvement of urethral closure mechanism under E2 and phytoestrogen administration that can be assumed to be beneficial for prevention or therapy of postmenopausal urge incontinence.

Puerarin: A Review of Pharmacological Effects

“…All of these changes elicited by puerarin were consistent with but weaker than 17β‐estradiol (E 2 ). These results demonstrate that puerarin could ameliorate urethral closure, which might be beneficial to the treatment of postmenopausal urge incontinence (Thielemann et al ., ). Puerarin markedly elevated weight, IGF‐1 and C3 expression, progesterone receptor mRNA levels in uterine, and the level of total antioxidant capacity in serum, whereas it attenuated MDA and lymphocytes DNA damage in ovx rats, suggesting that puerarin has uterotropic effects and might be an effective therapeutic agent against endometrial hyperplasia and premenstrual syndrome (Rachoń et al ., ; Tang et al ., ).…”

Loss of adenosine A2B receptor mediated relaxant responses in the aged female rat bladder; effects of dietary phytoestrogens

“…Ovariectomised rats are commonly used as a model to emulate human menopause (Diep and Contantinou 1999). The phytoestrogens that are present in soy bind both oestrogen receptors α and β (Morito et al 2001), and phytoestrogens have been shown to be effective as β-estradiol for improving bladder function in ovariectomised rats (Thielemann et al 2010).…”

Effect of Red Clover Isoflavones over Skin, Appendages, and Mucosal Status in Postmenopausal Women

“…No subjective improvement over urinary complaints were found in our study despite the fact that isoflavones have shown positive effects on the urethral sphincter mechanism in the animal model [49] and in patients with prostate carcinoma [50]. The latter clinical study [50], however, used a rather high isoflavone dose (200 mg/day for 6 months).…”