Identification of Streptococcus uberis Multilocus Sequence Types Highly Associated with Mastitis

Abstract

Multilocus sequence typing analysis of Streptococcus uberis has identified a cluster of isolates associated with clinical and subclinical mastitis and a cluster associated with cows with low somatic cell counts in their milk. Specific groups of genotypes (global clonal complex [GCC] sequence type 5s [ST5s] and GCC ST143s) were highly associated (P ‫؍‬ 0.006) with clinical and subclinical mastitis and may represent a lineage of virulent isolates, whereas isolates belonging to GCC ST86 were associated with low-cell-count cows. This study has, for the first time, demonstrated the occurrence of identical sequence types (ST60 and ST184) between different continents (Australasia and Europe) and different countries (Australia and New Zealand). The standardized index of association and the empirical estimation of the rate of recombination showed substantial recombination within the S. uberis population in Australia, consistent with previous multilocus sequence type analyses.

Genetic patterns of Streptococcus uberis isolated from bovine mastitis

“…Although the number of isolates of S. uberis examined in each herd was too small in some cases to draw firm conclusions, these data suggest the presence of a single clone that was transmitted either between cows or acquired from a common source. They support the findings from previous studies 1,5,10,11 , which reported that these events seemed to be uncommon. Phuektes et al 6 also reported the occurrence of a genetically diverse population; however, the authors demonstrated that two strains predominated at higher prevalence (>20 %) in two of the four herds.…”

Virulence-associated genes and molecular typing of Streptococcus uberisassociated with bovine mastitis in northern Thailand

“…We used a species- This technique can rapidly provide unambiguous results to detect S. uberis and can distinguish S. uberis from another phenotypically identical species, S. parauberis (20). This PCR protocol has been widely used to identify S. uberis isolated from mastitis cases prior to further genotyping methods (25,26). We found that the virulence gene pattern of hasA+has B+hasC+sua+gapC+lbp+pauA+oppF+mtuA was the most prevalent pattern among our S. uberis collection.…”

“…In the present study, we could not find any significant association of each virulenceassociated gene and disease status. A number of studies successfully reported a significant association of the presence of the hasA gene with particular strains of S. uberis that were identified to be related to subclinical and clinical bovine mastitis in the United Kingdom (28), New Zealand (29), and Australia (26). Even though we could not find any statistically significant association, the rate of hasA/B carriage among S. uberis isolated from clinical mastitis cases was higher than that among isolates from cases of IMI with low SCC (78.57% vs. 40%; P = 0.051).…”

The Comparison of Streptococcus agalactiae Isolated from Fish and Bovine using Multilocus Sequence Typing

“…Group B streptococcus (GBS) S. agalactiae is the cause of severe disease affecting fish and bovine (Tomita et al 2008). Different phenotypic characteristics of S. agalactiae have been noted GTTGGTCATGGTGAAGCACT GGTGTGTGCCATACTGATTT GATTAAGGAGTAGTGGCACG ATATCAACTCAAGAAAAGCT CGATTCTCTCAGCTTTGTTA CGATTCTCTCAGGCTTTGTTA CCGGCTACAGATGAACAATT AATAAAGCAATGTTTGATGG AGAGCAAGCTAATAGCCAAC AACATAGCAGAGCTCATGAT CTCGGAGGAACGACCATTAA GGTATCTTGACGCTTGAGGG CCAGGCTTTGATTTAGTTGA ACACTTCATGGTGATGGTTG ACTGTACCTCCAGCACGAAC ACAGCAGTCACAACCACTCC TTGAGATCGCCCATTGAAAT TGATGGAATTGAATGGCTATG YGATGGAATTGATGGCTATG AAGAAATCTCTTGTGCGGAT CTGATAATTGCCATTCCACG GCATTGTTCCCTTCATTATC ATATCAGCAGCAACAAGTGC GGGACTTCAACTAAACCTGC CTTGTAACAGTATCACCGTT ATCGCTGCTTTAATGGCAGA AATAGCTTGTTGGCTTGAAA TGACCTAGGTCATGAGCTTT 672 498 723 501 627 501 589 498 646 519 607 459 859 480 160 LUSIASTUTI ET AL.…”