Genetic patterns of Streptococcus uberis isolated from bovine mastitis

Reinoso, Elina; Lasagno, Mirta; Odierno, Liliana

doi:10.1016/j.ram.2015.02.001

Cited by 10 publications

(16 citation statements)

References 11 publications

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“…This indicates high population diversity among the S. uberis strains isolated from bovine mastitis in accordance with previous studies (LASAGNO et al, 2011;HAENNI et al, 2010;REINOSO et al, 2015;WANG et al, 1999WANG et al, , 2013. As discussed above, this diversity could be associated with environmental adaptations of this pathogen and its capacity to infect cows.…”

Section: Resultssupporting

confidence: 89%

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Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Loures¹,

Pereira²,

Carvalho-Castro³

et al. 2017

SCA

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ResumoA mastite é uma das doenças infecciosas mais onerosas em bovinos leiteiros em todos os continentes. Trata-se de uma doença de cunho multifatoral causada por diferentes microrganismos, incluindo vírus, leveduras, algas, parasitas e várias espécies de bactérias. Entre as bactérias, Streptococcus uberis destacase como um importante agente ambiental, responsável por uma grande variedade de infecções clínicas e subclínicas da glândula mamária, especialmente em sistemas de produção intensivos. Apesar da crescente importância de S. uberis na etiologia da mastite bovina, existem poucos estudos sobre a diversidade populacional e fatores de virulência deste patógeno em rebanhos leiteiros brasileiros.Os objetivos do presente estudo foram investigar as características de virulência e a epidemiologia molecular de S. uberis isolados de mastite bovina em rebanhos brasileiros, utilizando-se a PCR e a eletroforese em gel de campo pulsado (PFGE). Para tal, 46 amostras de S. uberis isoladas de mastite bovina em 26 rebanhos leiteiros de uma mesma mesorregião brasileira foram avaliadas quanto à diversidade populacional por eletroforese em gel de campo pulsado (PFGE), utilizando-se a enzima Smal. Além disso, foi realizada PCR para detectar os genes de virulência pauA e skc, que codificam ativadores de plasminogênio, e do gene sua, que codifica uma proteína de adesão às celulas epiteliais da glândula mamária. Nossos resultados mostraram uma alta diversidade genética na população estudada, com vários padrões de PFGE, e uma elevada frequência dos genes de virulência pesquisados: sua (100%), pauA (91%) e skc (91%). A alta frequência dos genes skc, pauA e sua sugere a importância destes fatores de virulência para S. uberis na colonização da glândula mamária bovina. Estudos moleculares e genéticos sobre este agente podem contribuir muito para melhorar o conhecimento sobre a patogenia, permitindo identificar moléculas que tenham papel relevante no estabelecimento da infecção, fornecendo informações importantes para a adoção de medidas mais eficazes para a prevenção e o controle da mastite bovina. AbstractMastitis is one of the most common and costly infectious diseases in dairy cattle worldwide. This is a multifactorial illness caused by different microorganisms, including virus, yeasts, algae, parasites, and several species of bacteria. Among these bacteria, Streptococcus uberis is an important environmental pathogen that is responsible for a large range of clinical and subclinical mammary infections, especially in intensively managed herds. Despite the increasing importance of this pathogen in the etiology of bovine mastitis, data on its virulence and diversity in Brazilian dairy herds are scarce. The aims of the present study were to investigate the virulence characteristics of S. uberis isolated from bovine mastitis and to assess the molecular epidemiology of the Brazilian isolates using pulsed-field gel electrophoresis (PFGE). In this work, 46 strains of S. uberis isolated from bovine mastitis from 26 Brazilian dairy herds were ...

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Section: Resultssupporting

confidence: 89%

“…Reinoso et al (2015) evaluated the genotypic relationships among 40 S. uberis strains isolated from bovine mastitis by PFGE. They identified 17 different PFGE patterns, showing that distinct strains were present within and among the herds.…”

Section: Resultsmentioning

confidence: 99%

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Loures¹,

Pereira²,

Carvalho-Castro³

et al. 2017

SCA

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“…Genotypic relationships among 68 S uberis isolates were previously assayed by PFGE (Reinoso et al., 2015). Macro restriction analysis revealed thirty-seven PFGE profiles.…”

Section: Resultsmentioning

confidence: 99%

“…S. uberis, E. faecalis, S. agalactiae and S. dysgalactiae strains were additionally confirmed using molecular techniques as 16S rDNA RFLP (Jayarao et al., 1992; Khan et al., 2003). Furthermore, S. uberis strains were previously genotypically characterized by using pulsed-field gel electrophoresis (PFGE) and PFGE profiles were identified in separate studies (Reinoso et al., 2015; Moliva et al., 2017).…”

Section: Methodsmentioning

confidence: 99%

Screening of bacteriocin associated genes of Streptococcus uberis strains

Lasagno

Navarro

Moliva

et al. 2019

Heliyon

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A wide variety of contagious and environmental bacteria can cause bovine mastitis worldwide. Antibiotic therapy is currently used for the treatment of the disease, although its intensive use leads to the emergence of resistant strains. Bacteriocins arise as potential antibacterial option for mastitis treatment. The aim of this work was to analyze bacteriocin associated genes as Streptococcus uberis ubericin A (ubaA), ubericin A immunity protein (ubaI), uberolysin A (ublA), Lantibiotic nisin-U (nsuA and nsuB) in 68 S uberis strains. Furthermore, the ability of the strains to inhibit important mastitis pathogens was assayed. Results showed that genes were present in combination and all the strains carried at least one gene. Seven bacteriocion associated gene patterns were identified. S. uberis strains were able to inhibit different mastitis pathogens and the greatest inhibition was observed in CNS strains. The results obtained provide new insights on antibacterial activity produced by S. uberis strains against different mastitis pathogens and could contribute to the development of strategies to treat intramammary infections.

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“…Different studies about biofilm production by mastitis strains have been reported [104,105]. Previously, we studied the biofilm formation ability of S. uberis strains the effects of several factors as additives and bovine milk compounds on biofilm production and the genetic variation among S. uberis isolates to establish relationship between virulence profiles and PFGE patterns [14,106,107]. The results showed that S. uberis isolates had a notable ability to produce biofilm at different degrees [14].…”

Section: Bovine Mastitis and Biofilmmentioning

confidence: 99%

Bovine Mastitis Caused By Streptococcus uberis: Virulence Factors and Biofilm

Reinoso¹

2017

J Microb Biochem Technol

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Genetic patterns of Streptococcus uberis isolated from bovine mastitis

Cited by 10 publications

References 11 publications

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Genetic diversity and virulence genes in Streptococcus uberis strains isolated from bovine mastitis

Screening of bacteriocin associated genes of Streptococcus uberis strains

Bovine Mastitis Caused By Streptococcus uberis: Virulence Factors and Biofilm

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