Screening of bacteriocin associated genes of Streptococcus uberis strains

Lasagno, Mirta; Navarro, María de los Ángeles Zardón; Moliva, Melina Vanesa; Reinoso, Elina

doi:10.1016/j.heliyon.2019.e02393

Cited by 10 publications

(9 citation statements)

References 18 publications

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“…In cattle, the most common target group for the use of bacteriocins includes pathogens responsible for the infection of mammary glands [ 147 ]. Godoy-Santos et al [ 148 ] showed an inhibitory effect of bovicin, a bacteriocin produced by Streptococcus equinus , against most Gram-positive isolates from mastitis but not against Gram-negative strains, including E. coli .…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

“…The research conducted by Carson et al [ 149 ] confirmed that bacteria that are the main cause of mastitis (non- Aureus staphylococci ) may also be an important group of producers of bacteriocin that effectively inhibits not only animal-origin S. aureus but also human-origin methicillin-resistant S. aureus strains (MRSA). Lasagno et al [ 147 ] showed the presence of genes encoding more than one bacteriocin in Streptococcus uberis strains isolated from mastitis; moreover, these strains showed inhibitory activity against other species of bacteria causing mastitis, especially coagulase-negative staphylococci.…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

See 1 more Smart Citation

Last Call for Replacement of Antimicrobials in Animal Production: Modern Challenges, Opportunities, and Potential Solutions

Nowakiewicz

Zięba²,

Gnat

et al. 2020

Antibiotics

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The constant market demand for easily available and cheap food of animal origin necessitates an increasing use of antibiotics in animal production. The alarming data provided by organizations monitoring drug resistance in indicator and pathogenic bacteria isolated from humans and animals indicate a possible risk of a return to the preantibiotic era. For this reason, it seems that both preventive and therapeutic measures, taken as an alternative to antimicrobials, seem not only advisable but also necessary. Nevertheless, the results of various studies and market analyses, as well as difficulties in the implementation of alternative substances into veterinary medicine, do not guarantee that the selected alternatives will completely replace antimicrobials in veterinary medicine and animal production on a global scale. This publication is a brief overview of the drug resistance phenomenon and its determinants, the steps taken to solve the problem, including the introduction of alternatives to antimicrobials, and the evaluation of some factors influencing the potential implementation of alternatives in animal production. The review also presents two groups of alternatives, which, given their mechanism of action and spectrum, are most comparable to the effectiveness of antibiotics, as emphasized by the authors.

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Section: Antimicrobial Peptidesmentioning

confidence: 99%

Section: Antimicrobial Peptidesmentioning

confidence: 99%

Last Call for Replacement of Antimicrobials in Animal Production: Modern Challenges, Opportunities, and Potential Solutions

Nowakiewicz

Zięba²,

Gnat

et al. 2020

Antibiotics

View full text Add to dashboard Cite

show abstract

“…Previous studies have used PCR screening to investigate the distribution of biosynthetic gene clusters associated with bacteriocin production (Lasagno et al . 2019; Sabino et al . 2019; Campanero et al .…”

Section: Discussionmentioning

confidence: 99%

PCR screening reveals abundance of bovicin‐like bacteriocins among ruminal Streptococcus spp. isolated from beef and dairy cattle

et al. 2021

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Aims To investigate the inhibitory activity and the distribution of biosynthetic genes encoding bovicin‐like bacteriocins among ruminal Streptococcus isolated from beef and dairy cattle. Methods and Results Most isolates were classified as Streptococcus equinus and Streptococcus lutetiensis based on 16S rRNA sequencing. The antimicrobial activity of 150 ruminal streptococci isolated from beef and dairy cattle were tested by deferred inhibition assays and their genetic diversity was characterized by BOX‐PCR. The frequency of biosynthetic genes associated with the biosynthesis of bovicin‐like bacteriocins (bovicin HC5 and bovicin 255) was investigated by PCR screening. Approximately 33% of the ruminal streptococci isolated from Nellore heifers showed inhibitory activity in vitro with the majority harbouring genes for bacteriocin biosynthesis. In contrast, streptococci from Holstein cows showed limited inhibitory activity and a lower frequency of bacteriocin biosynthetic genes. Conclusions Streptococcus from the rumen of beef and dairy cattle exhibit remarkable differences in inhibitory activity and distribution of genes associated with the biosynthesis of prototypical bovicins (bovicin HC5 and bovicin 255). Significance and Impact of the Study Our findings demonstrate that bovicin HC5 is distributed among ruminal streptococci from different breeds of cattle. The high degree of conservation of the bovicin HC5 structural gene among strains of ruminal streptococci suggests that random genetic drift is not a dominant force in the evolution of this bacteriocin.

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“…Virulence genes screened by PCR DNA extraction was carried out according to Lasagno et al (2019). The presence of virulence genes, gapC, hasABC, lbp, pauA and sua was assayed by PCR.…”

Section: Intracellular Survival Assaymentioning

confidence: 99%

Capacity of adherence, invasion and intracellular survival of Streptococcus uberis biofilm-forming strains

Moliva

Campra

Ibañez

et al. 2022

Journal of Applied Microbiology

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Aim: Nine Streptococcus uberis strains with different biofilm-forming profiles in relation to their capacity of adherence and invasion to MAC-T cell lines were examined. Additionally, virulence genes were also linked to adherence and invasion.Methods and Results: All S. uberis were able to adhere and invade the cells at different levels. UB56 strain showed the highest percentage of internalization (3.65%) and presented a moderate level of adhesion (4.6 × 10 6 ). In contrast, UB152, the most adherent strain (8.7 × 10 6 ) showed a low capacity to internalize (0.65%). Eight strains were able to persist intracellularly over 96 h regardless of their adherence or invasion level. Statistical analysis between biofilm-forming ability and the adhesion capacity showed no significant differences. Presence of virulence genes involved in the adhesion process (gapC, hasABC, lbp, pauA and sua) showed that the strains harboured different genes and seven patterns could be observed. Conclusion:Statistical analysis showed no correlation between the virulence gene patterns and the adhesion capacity or the percentage of internalization. Biofilmforming ability did not influence the invasion capacity. Likewise, adherence and invasion capacity may be strain dependent.Significance and Impact of the Study: Findings from this study provide new insights on biofilm and invasion capacity of S. uberis strains. Results could help to design adequate control strategies.

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Screening of bacteriocin associated genes of Streptococcus uberis strains

Cited by 10 publications

References 18 publications

Last Call for Replacement of Antimicrobials in Animal Production: Modern Challenges, Opportunities, and Potential Solutions

Last Call for Replacement of Antimicrobials in Animal Production: Modern Challenges, Opportunities, and Potential Solutions

PCR screening reveals abundance of bovicin‐like bacteriocins among ruminal Streptococcus spp. isolated from beef and dairy cattle

Capacity of adherence, invasion and intracellular survival of Streptococcus uberis biofilm-forming strains

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