Identification of<i>Escherichia coli</i>and<i>Salmonella enterica</i>organisms with reduced susceptibility to ceftriaxone from fecal samples of cows in dairy herds

Heider, Luke C.; Funk, Julie A.; Hoet, Armando E.; Meiring, Richard W.; Gebreyes, Wondwossen A.; Wittum, Thomas E.

doi:10.2460/javma.234.6.799

Cited by 11 publications

(22 citation statements)

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“…Similar recovery rates have been reported in U.S. dairy cattle populations and appear to be increasing (2,15,16). We also recovered two Salmonella isolates from two barns belonging to the same company in Ohio representing a single clonal strain that carried bla CMY-2 on IncI1 plasmids, which suggests that enteric bacteria can serve as a reservoir of extended-spectrum cephalosporin resistance genes for pathogens.…”

supporting

confidence: 60%

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Mollenkopf

Mirecki

Daniels

et al. 2013

Appl Environ Microbiol

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confidence: 60%

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Mollenkopf

Mirecki

Daniels

et al. 2013

Appl Environ Microbiol

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“…Amplification of another extended-spectrum-cephalosporin resistance gene, bla CMY-2 , in fecal E. coli has been associated with ceftiofur use in livestock (19,28,47). However, others have reported no association of ceftiofur use with extended-spectrum-cephalosporin resistance or simply a reduction in susceptible bacteria allowing for these rare resistant phenotypes to be detected (10,16,30,42). The frequent use of ceftiofur in dairy herds may make the detection of a true association between ceftiofur administration and the recovery of bla CTX-Mpositive fecal E. coli difficult.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…We previously reported that ceftiofur was used by 61% of Ohio dairy farms in 2006 (47) and 88% of such farms in 2009 (16), suggesting that ceftiofur use by dairy producers may have increased. Over the same time period, we recovered E. coli with bla CMY-2 from 67% of herds and 34% of individual cow fecal samples in 2006 (47) and from 92% of herds and 70% of cows in 2009 (16). With on-farm use of ceftiofur potentially contributing to the emergence, maintenance, and dissemination of extended-spectrum-cephalosporin resistance genes within dairy herds, our observed increase in bla CMY-2 prevalence in dairy populations concurrent with increasing ceftiofur use may portend a similar dissemination of bla CTX-M in U.S. livestock.…”

Section: Discussionmentioning

confidence: 99%

“…We used a two-phase enrichment in supplemented tetrathionate (TTB) and Rappaport-Vassiliadis R10 (RV) broths followed by differential selection on xylose-lysine-Tergitol 4 agar (XLT-4) (16,31). From each sample, bacteria from a single black colony on XLT-4 were isolated on MacConkey agar and confirmed as Salmonella by using standard biochemical reactions, including triple sugar iron (TSI) agar, urea broth, and polyvalent antisera testing.…”

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confidence: 99%

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Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Mollenkopf

Weeman

Daniels

et al. 2012

Appl Environ Microbiol

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ABSTRACTblaCTX-Mbeta-lactamases confer resistance to critically important cephalosporin drugs. Recovered from both hospital- and community-acquired infections,blaCTX-Mwas first reported in U.S. livestock in 2010. It has been hypothesized that veterinary use of cephalosporins in livestock populations may lead to the dissemination of beta-lactamase-encoding genes. Therefore, our objectives were to estimate the frequency and distribution of coliform bacteria harboringblaCTX-Min the fecal flora of Ohio dairy cattle populations. In addition, we characterized the CTX-M alleles carried by the isolates, their plasmidic contexts, and the genetic diversity of the bacterial isolates themselves. We also evaluated the association between ceftiofur use and the likelihood of recovering cephalosporinase-producing bacteria. Thirty fresh fecal samples and owner-reported ceftiofur use data were collected from each of 25 Ohio dairy farms. Fecal samples (n= 747) yielded 70blaCTX-M-positiveEscherichia coliisolates from 5/25 herds, 715blaCMY-2E. coliisolates from 25/25 herds, and 274Salmonellaspp. from 20/25 herds. The within-herd prevalence amongblaCTX-M-positive herds ranged from 3.3 to 100% of samples. Multiple pulsed-field gel electrophoresis (PFGE) patterns, plasmid replicon types, and CTX-M genes were detected. Plasmids with CTX-M-1, -15, and -14 alleles were clonal by restriction fragment length polymorphism (RFLP) within herds, and specific plasmid incompatibility group markers were consistently associated with eachblaCTX-Mallele. PFGE of total bacterial DNA showed similar within-herd clustering, with the exception of one herd, which revealed at least 6 different PFGE signatures. We were unable to detect an association between owner-reported ceftiofur use and the probability of recoveringE. colicarryingblaCTX-MorblaCMY-2.

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Antimicrobial‐resistant genes associated with Salmonella spp. isolated from human, poultry, and seafood sources

Adesiji

Deekshit

Karunasagar

2014

Food Science & Nutrition

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Antimicrobial-resistant salmonellosis is a significant public health concern globally. A study was conducted to screen for Salmonella species from a total of 120 samples, of which 50 were retail meat samples purchased from five randomly selected sales outlets in the city of Mangalore, India. Twenty poultry fecal materials freshly voided before slaughter were obtained with sterile spatula and placed in sterile sealable polythene envelopes, and 20 clams were purchased from the estuaries of Nethravathi and Kankarnady market. In addition, 30 clinical isolates from Nigeria suspected to be Salmonella by only cultural characterization were also included in the study. In all, 30 samples—6 poultry, 8 seafood, and 16 Salmonella isolates from clinical samples—were confirmed positive by PCR and used in this study. The disk-diffusion test was performed to determine the zone of inhibition, and detection of resistance genes was tested by PCR targeting various antimicrobial genes. Resistance to tetracycline (TET), cotrimoxazole, nalidixic acid, nitrofurantion, and piperacillin/tazobactin was found in 66.7%, 60%, 53.3%, 50% and 50% of the isolates, respectively. About 60–100% of MDR isolates possessed antibiotic-resistant genes, of the tetracyclines resistant isolates, 20 (100%) 6 (30%), 7 (35%), and 10 (50%) carried tetA, tetB, tetC, and tetG genes, respectively. Of 18 cotrimoxazole-resistant strains, 18 (100%), 14 (77.7%), and 4 (22.2%) had sul1, sul2, and sul3 genes, respectively. Of the 14 multidrug-resistant isolates tested, 8 (61%) and 9 (69%) were positive for cmlA and cmlB genes, respectively, 10 (1.4%) tested positive for aph(3)11a genes, 8 (57%) tested positive for aac(3)lla, while none was positive for the aac6 gene. The results show the presence of antibiotic-resistant Salmonella spp. in food samples from India and in human samples from Nigeria.

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Identification ofEscherichia coliandSalmonella entericaorganisms with reduced susceptibility to ceftriaxone from fecal samples of cows in dairy herds

Cited by 11 publications

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Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Antimicrobial‐resistant genes associated with Salmonella spp. isolated from human, poultry, and seafood sources

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