Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Mollenkopf, Dixie F.; Mirecki, Jennifer M.; Daniels, Joshua B.; Funk, Julie A.; Henry, Steven C.; Hansen, Glenn E.; Davies, Peter; Donovan, Tara; Wittum, Thomas E.

doi:10.1128/aem.03169-12

Cited by 10 publications

(10 citation statements)

References 18 publications

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“…Disturbingly Huang and co-workers isolated a number of pig strains of E coli carrying qnrS1 (fluoroquinolone resistance) and bla CTX-M-14 on a multi-drug resistant plasmid [48]. CTX-M producing Klebsiella pneumoniae were isolated from USA pig faeces [58] and transfer of E. coli plasmids encoding bla CTX-M-1 between pigs and piggery workers has been documented in Denmark [59]. Of particular concern is the detection of carbapenemases in bacteria from pigs and piggery environments as carbapenems are not used in pigs.…”

Section: Escherichia Colimentioning

confidence: 99%

Impact of antibiotic use in the swine industry

Barton

2014

Current Opinion in Microbiology

176

119

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Antibiotic resistance in bacteria associated with pigs not only affects pig production but also has an impact on human health through the transfer of resistant organisms and associated genes via the food chain. This can compromise treatment of human infections. In the past most attention was paid to glycopeptide and streptogramin resistance in enterococci, fluoroquinolone resistance in campylobacter and multi-drug resistance in Escherichia coli and salmonella. While these are still important the focus has shifted to ESBL producing organisms selected by the use of ceftiofur and cefquinome in pigs. In addition Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) suddenly emerged in 2007. We also need to consider multi-resistant strains of Streptococcus suis. Environmental contamination arising from piggery wastewater and spreading of manure slurry on pastures is also a growing problem.

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Section: Escherichia Colimentioning

confidence: 99%

Impact of antibiotic use in the swine industry

Barton

2014

Current Opinion in Microbiology

176

119

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“…The AmpC bla CMY‐2 and the ESBL bla CTX‐M each confer resistance to ESCs including ceftiofur (Ewers et al., ) and are frequently carried on mobile plasmids which can be readily transmitted between bacterial species (Winokur et al., ). The common use of ceftiofur in livestock populations has been associated with the widespread dissemination of these ESC resistance genes in the faecal flora of livestock (Heider et al., ; Wittum et al., ; Mollenkopf et al., ) and in fresh retail meat products (Mollenkopf et al., , ).…”

Section: Introductionmentioning

confidence: 99%

Extended‐Spectrum β‐lactam Resistance in the Enteric Flora of Patients at a Tertiary Care Medical Centre

Landers

Mollenkopf

Faubel

et al. 2016

Zoonoses and Public Health

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The dissemination of Enterobacteriaceae expressing resistance to extended-spectrum cephalosporins, which are therapeutically used in both human and veterinary medicine, is of critical concern. The normal commensal flora of food animals may serve as an important reservoir for the zoonotic food-borne transmission of Enterobacteriaceae harbouring β-lactam resistance. We hypothesized that the predominant AmpC and ESBL genes reported in US livestock and fresh retail meat products, bla and bla , would also be predominant in human enteric flora. We recovered enteric flora from a convenience sample of patients included in a large tertiary medical centre's Clostridium difficile surveillance programme to screen for and estimate the frequency of carriage of AmpC and ESBL resistance genes. In- and outpatient diarrhoeic submissions (n = 692) received for C. difficile testing at the medical centre's clinical diagnostic laboratory from July to December, 2013, were included. Aliquoted to a transport swab, each submission was inoculated to MacConkey broth with cefotaxime, incubated at 37°C and then inoculated to MacConkey agars supplemented with cefoxitin and cefepime to select for the AmpC and ESBL phenotypes, with bla and bla genotypes confirmed by PCR and sequencing. From the 692 diarrhoeic submissions, our selective culture yielded 184 isolates (26.6%) with reduced susceptibility to cefotaxime. Of these, 46 (6.7%) samples harboured commensal isolates carrying the AmpC bla . Another 21 (3.0%) samples produced isolates harbouring the ESBL bla : 19 carrying CTX-M-15 and 2 with CTX-M-27. Our results indicate that β-lactam resistance genes likely acquired through zoonotic food-borne transmission are present in the enteric flora of this hospital-associated population at lower levels than reported in livestock and fresh food products.

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“…Since then, CTX-M-type ESBLs have been found in E. coli collected from healthy dairy calves in the western U.S. (Davis et al, 2011) and from 5 of 20 dairy farms in Ohio (Mollenkopf et al, 2012); in clinical Salmonella enterica isolates from swine in Minnesota and from turkeys in 4 states (Wittum et al, 2012); in E. coli from swine finishing barns in Michigan and Ohio and in Klebsiella pneumoniae from swine in Illinois (Mollenkopf et al, 2013). …”

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confidence: 99%