Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Mollenkopf, Dixie F.; Weeman, Matthew; Daniels, Joshua B.; Abley, Melanie; Mathews, Jennifer; Gebreyes, Wondwossen A.; Wittum, Thomas E.

doi:10.1128/aem.00373-12

Cited by 54 publications

(57 citation statements)

References 52 publications

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“…To select for bla CTX-M -and bla CMY-2 -associated phenotypes, samples from all farms were processed as previously described (22). Briefly, feces (5 g) were incubated in 45 ml nutrient broth (Hardy Diagnostics, Santa Maria, CA, USA) supplemented with cefotaxime (2 g/ml) at 37°C for 18 to 24 h without shaking.…”

Section: Methodsmentioning

confidence: 99%

“…We detected the presence of bla CTX-M and bla CMY-2 genes using previously published protocols (8,21,22 Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…Cefepime was used to screen for a bla CTX-M phenotype and cefoxitin for a bla CMY-2 phenotype (22). Bacterial isolates grown on cefepime-and cefoxitin-supplemented media were tested for the presence of bla CTX-M and bla CMY-2 , respectively.…”

Section: Methodsmentioning

confidence: 99%

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Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms

Davis

Sischo

Jones

et al. 2015

Appl Environ Microbiol

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c Enterobacteriaceae-associated bla CTX-M genes have become globally widespread within the past 30 years. Among isolates from Washington State cattle, Escherichia coli strains carrying bla CTX-M (CTX-M E. coli strains) were absent from a set of 2008 isolates but present in a set of isolates from 2011. On 30 Washington State dairy farms sampled in 2012, CTX-M E. coli prevalence was significantly higher on eastern than on northwestern Washington farms, on farms with more than 3,000 adult cows, and on farms that recently received new animals. The addition of fresh bedding to calf hutches at least weekly and use of residual fly sprays were associated with lower prevalence of CTX-M E. coli. In Washington State, the occurrence of human pathogens carrying bla CTX-M genes preceded the emergence of bla CTX-M -associated E. coli in cattle, indicating that these resistance determinants and/or their bacterial hosts may have emerged in human populations prior to their dissemination to cattle populations. E mergence and dissemination of antibiotic resistance traits in Gram-negative bacteria can occur rapidly and across wide geographic distances (1). Bacterial strains carrying the bla CTX-M family of extended-spectrum ␤-lactamase (ESBL) genes have spread globally in less than 30 years (2, 3). Unlike other families of ESBL genes which result from point mutations in preexisting, narrower-spectrum ␤-lactamase genes (bla TEM-1 and bla SHV ) (4), bla CTX-M ␤-lactamase genes originated from chromosomal genes of Kluyvera species (2). The earliest Escherichia coli plasmid-associated bla CTX-M was detected from laboratory dogs used in pharmaceutical research in Japan in 1986, followed by isolations from human patients in Munich, Germany (1989), Argentina (1989), France (1989), and Poland (1996) (2). In North America, bla CTX-Mbearing Escherichia coli strains (CTX-M E. coli strains) were detected in humans in Canada in 2000 (5) and in the United States between 2001 and 2002 (6). By 2007 in the United States, 80% of 15 geographically dispersed medical centers reported E. coli or Klebsiella pneumoniae infections with associated bla CTX-M genes (7). In Washington State, the earliest reported clinical human E. coli strain carrying bla CTX-M was isolated in 2001 in Seattle (6). Currently, CTX-M enzymes are considered the most prevalent ESBLs in isolates of E. coli, K. pneumoniae, and Proteus mirabilis associated with human infections globally (8).In the United States, dairy cattle-associated CTX-M E. coli strains were first reported by Wittum and others in Ohio in 2009 (9). This Ohio research group later reported CTX-M in Salmonella enterica isolates from equine, swine, and turkey sources from six different states (10) and from swine finishing barns in 5 states (11). In the current study, we first tested E. coli isolates from Washington State dairy cattle banked from several earlier research projects to determine the time window of CTX-M E. coli emergence in this region and host species. The results of this retrospective study indicated rece...

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Section: Methodsmentioning

confidence: 99%

“…We detected the presence of bla CTX-M and bla CMY-2 genes using previously published protocols (8,21,22 Table 1.…”

Section: Methodsmentioning

confidence: 99%

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Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms

Davis

Sischo

Jones

et al. 2015

Appl Environ Microbiol

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“…In fact, these intensively managed production systems may produce an environment in which there is effectively a common flora. As a result, selection pressure applied to an individual may hasten the emergence of resistant pathogens or resistance genes that can be quickly and easily transmitted to others in the same population, often by means of horizontally transmissible genetic elements (4,14). Fecal flora from these animals may then contaminate carcasses during processing, which can result in zoonotic food-borne transmission (1,13).…”

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The Challenge of Regulating Agricultural Ceftiofur Use To Slow the Emergence of Resistance to Extended-Spectrum Cephalosporins

Wittum

2012

Appl Environ Microbiol

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“…e previously reported the bla CTX-M gene in fecal Escherichia coli from cattle in Ohio (1), described their distribution among dairy herds in Ohio (2), and reported the recovery of Salmonella harboring bla CTX-M from livestock diagnostic submissions (3). bla CTX-M has been recovered from livestock pathogens and commensal flora in Europe and Asia (4-7) and from sporadic human cases of salmonellosis in the United States (8)(9)(10).…”

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confidence: 99%

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

Mollenkopf

Mirecki

Daniels

et al. 2013

Appl Environ Microbiol

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Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Cited by 54 publications

References 52 publications

Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms

Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms

The Challenge of Regulating Agricultural Ceftiofur Use To Slow the Emergence of Resistance to Extended-Spectrum Cephalosporins

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

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Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle

Cited by 54 publications

References 52 publications

Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla CTX-M on Washington State Dairy Farms

Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla CTX-M on Washington State Dairy Farms

The Challenge of Regulating Agricultural Ceftiofur Use To Slow the Emergence of Resistance to Extended-Spectrum Cephalosporins

Escherichia coli and Klebsiella pneumoniae Producing CTX-M Cephalosporinase from Swine Finishing Barns and Their Association with Antimicrobial Use

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Product

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Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms

Recent Emergence of Escherichia coli with Cephalosporin Resistance Conferred by bla _CTX-M on Washington State Dairy Farms