Identification of Glutathione S-Transferase Genes in Hami Melon (Cucumis melo var. saccharinus) and Their Expression Analysis Under Cold Stress

Abstract

As a group of multifunctional enzymes, glutathione S-transferases (GSTs) participate in oxidative stress resistance and cellular detoxification. Here, we identified 39 CmGST genes with typical binding sites from the Hami melon genome, and they can be classified into seven subfamilies. Their molecular information, chromosomal locations, phylogenetic relationships, synteny relationships, gene structures, protein–protein interactions, structure of 3-D models, and expression levels under cold stress were analyzed. Expression analysis indicates that cold-tolerant Jia Shi-310 (JS) had higher GST enzyme activities and expression levels of 28 stress-related genes under cold stress. Some CmGSTs belonging to Tau, Phi, and DHAR classes play significant roles under cold stress, and they could be regarded as candidate genes for further studies. The present study systematically investigated the characterization of the Hami melon GST gene family, extending our understanding of Hami melon GST mediated stress-response mechanisms in this worldwide fruit.

Genome-wide identification and expression analysis of glutathione S-transferase gene family to reveal their role in cold stress response in cucumber

“…The results implied that the CsGSTs were enriched in “glutathione metabolism,” “arachidonic acid metabolism,” and the “tosine metabolism” pathway, and the “glutathione metabolism” pathway played a vital role in resisting cold stress. The results are consistent with a previous study that revealed the involvement of CmGSTs in the “glutathione metabolism” pathway during cold stress response in Hami melon ( Song et al, 2021 ). Therefore, we speculated that CsGSTs could bind to and activate the thiol group of GSH, and the S-glutathionylated xenobiotics would be sequentially processed to participate in the cold stress response.…”

Computational genomics insights into cold acclimation in wheat

“…Plant adaptation to low temperature not only induces lipid desaturation in cellular membranes but also generation of reactive oxygen species (ROS) and changes in redox state ( Murata and Los, 1997 ; Wallis and Browse, 2002 ). The multifunctional enzymes glutathione S-transferases (GSTs) participate in oxidative stress resistance and cellular detoxification and highly associated with cold stress of Hami melon ( Song W. et al, 2021 ) and pumpkin ( Abdul Kayum et al, 2018 ). There are 92 GSTs or GSTs like in the DEGs list, the majority of them, including a key hub gene in the WHGs subnetwork (TraesCS1B03G0752200), are significantly upregulated by cold, especially in Norstar and spring Norstar.…”

Genome-wide identification and characterization of glutathione S-transferase gene family in Musa acuminata L. AAA group and gaining an insight to their role in banana fruit development

“…Glutathione S-transferases (GSTs) are an inbuilt antioxidant enzymatic defense system of plants, that works downstream of Cyt P450. GST enzyme superfamily is primarily occupied in scavenging diverse chemical compounds present in the soil or in the environment by conjugating glutathione (GSH), a natural non enzymatic antioxidant defense system in plants (Song et al 2021), to a hydrophobic substrate to make chemical compound more hydrophilic to be expelled from the cell through vacuole (Basantani et al 2007). GSTs are the key enzymes in plant growth and development, secondary metabolism, anythocyanin accumulation (Shao et al 2021), signal transduction pathways (Nianiou-Obeidat et al 2017), tetrapyrole metabolism and retrograde signaling (Sylvestre-Gonon et al 2020), detoxi cation of reactive carbonyl species (RCS) (Mano et al 2019) and against various biotic and abiotic stresses.…”

“…It is functionally a versatile protein family involved in plant growth and development, signal transduction pathways, retrograde signaling, biotic and abiotic stress management, tetrapyrole signaling, hormone signaling etc. To date genome wide identi cation and characterization of GST gene family have been performed in variety of plants like hami melon(Song et al 2021), melon(Wang et al 2020), radish(Gao et al 2020), medicago(Hasan et al 2021) etc. The comprehensive genome wide identi cation of a gene family is precise and more signi cant using the whole genome information.…”