Identification of Gh|alui and Ghr|alui Genes Polymorphisms in Indonesian Buffalo

Abstract

Growth hormone (GH) is an anabolic hormone which sintesized and secreted by somatrotop cell in pituitary anterior lobe. GH exert its effect on growth and metabolism by interacting with a specific receptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested as candidate gene for traits related to meat production in Bovidae. The objectives of this study were to identify polymorphism of GH and GHR genes in buffalo. The 452 DNA samples buffalo were collected from five populations in Indonesia (Siborong-Borong-Medan (65), Lebak-Banten (29), PandeglangBanten (180), Semarang-Central Java, and Mataram-West Nusa Tenggara (103)). A gene fragment of the GH|AluI gene at 432 bp located on exon 3 and GHR|AluI gene at 298 bp on exon 10 were successfully amplified by using the techniques of a PCR (polymerase chain reaction) and genotyped by PCR-RFLP (restriction fragment length polymorphism) then -SSCP (single strand conformation polymorphism). The results showed no polymorphisms were detected in these genes. All buffaloes tested had LL genotype for locus GH|AluI and AA genotype for locus GHR|AluI.

Growth Hormone Gene Polymorphism and Its Association with Partial Cumulative Milk Yields of Holstein Friesian Dairy Cattle

“…Annelaling temperature is a temperature allowing the primers attaching on DNA templates during a PCR process. The annealing temperature 60 °C for 1 min in this study was accordance with those of some previous studies (Balog et al, 2009;Mohammadabadi et al, 2010, andAndreas et al, 2010).…”

“…Extraction procedure followed the phenol-chloroform method that was modified by Andreas et al (2010), with the following procedures:…”

The Variability of Growth Hormone Gene Associated with Ultrasound Imaging of Longissimus dorsi Muscle and Perirenal Fat in Rabbits

“…Blood samples were then mixed with 96% ethanol. The process of DNA isolation used phenol-chloroform method (Sambrook et al, 1989) that was modified by Andreas et al (2010). Genomic DNA was stored at -20 o C until amplification with polymerase chain reaction (PCR).…”

Genetic Polymorphisms of the Coding Region (Exon 6) of Calpastatin in Indonesian Sheep

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