Identification of genetic markers of resistance to Potato virus Y and Golden potato cyct nematode in promising potato varieties by means of PCR

Abstract

The study used genetic material of 13 field-grown potato varieties: Avgustin, Belmonda, Dachniy, Kazachok, Koroleva Anna, Labella, Laperla, Lilly, Red Lady, Sante, Smak, Yubilyar, Yantar and 6 varieties grown in vitro – Adretta, Zhukovskyi rannyi, Khazachiok, Sante, Smak, Yantar. The development of molecular genetic methods for identification of genetic markers of resistance to Potato virus Y and Golden potato cyct nematode was carried out. Meteor variety was used as a positive control due to having the desired genes in its genome. After screening testing of 16 promising potato varieties for resistance to Potato virus Y YES 3A marker of Rysto gene have not been identified. Golden potato cyct nematode resistance H1 gene markers 57R and N195 have been identified in Belmonda, Zhukovskyi rannyi, Koroleva Anna, Labella, Laperla, Lilly, Red Lady, Sante, Smak, and Yubilyar varieties.