Identification of fungal ( Magnaporthe grisea ) stress-induced genes in wild rice ( Oryza minuta )

Abstract

Introgression lines derived from Oryza minuta and O. sativa subsp. japonica var. Junambyeo were crossed for a mapping of the population composed of 112 recombinant lines to identify putative QTLs against rice blast disease using the percentage of diseased leaf area. By using 148 Sequence Tagged Site (STS) and Single Sequence Repeat (SSR) markers, five QTLs on chromosomes 6, 7, 9 and 11 and seven epistatic QTLs were identified against two blast isolates (KI307 and KI209). Of them two QTLs (qKI307-2 and qKI209-3) shared a similar position on chromosome 11. O. minuta introgression contributed the resistance allele for all of these QTLs. Combined phenotypic variations by QTL and (E-QTL) accounted for 56.9% against KI307, and 53.4% against KI209. Each QTL could account for the resistance variation between 11 and 24.6%. The resistance from wild introgressions was attributable to a combination of QTLs and epistatic effects between different loci, capable of inducing hypersensitive reactions. Our findings are in support of the strategy of pyramiding major QTLs to develop improved rice varieties with durable broad spectrum resistance against the blast fungus.

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

“…Thus, the resulting differential cDNA In this study, six contigs (3, 4, 6, 7, 32 and 33) representing nearly 37 % of the EST sequences showed no significant similarity to known protein sequences, reflecting the necessity of sequence annotation in fungi. This high frequency of orphan sequences is similar to that observed in previous fungal EST projects (Shim et al 2004;Viaud et al 2005) and is consistent with findings of other fungal EST studies on different plant pathogens (Lakshman et al 2012;Neumann and Dobinson 2003;Trail et al 2003;Viaud et al 2005). However, the lack of similarity of these EST sequences to entries in protein databases could be due to the fact that they might be derived from 5′ and 3′ un-translated regions (UTRs) (Skinner et al 2001).…”

Novel Bifunctional Nucleases, OmBBD and AtBBD1, Are Involved in Abscisic Acid-Mediated Callose Deposition in Arabidopsis

“…On the other hand, the BPH causes mechanical wounding by chewing, but it does not kill the host cells as a biotrophic insect (Sogawa, 1976;Kim and Sohn, 2005). In an earlier study, we screened O. minuta transcriptomes from wound-, BPH-, or rice blasttreated leaves and normal conditioned leaves in a high-throughput system to identify useful defenserelated genes from O. minuta (Cho et al, 2004a(Cho et al, , 2004b(Cho et al, , 2005Shim et al, 2004). In the study reported here, we identified a novel gene induced by wound-, BPH-, and M. oryzae in O. minuta.…”

“…A conidia suspension of the rice blast Magnaporthe oryzae (5 3 10 4 conidia mL 21 in 0.1% Tween 20; a mixture of four races of M. oryzae, namely KJ101, KJ102, KJ197, and KI409), which causes severe disease symptom in O. sativa but not in O. minuta, was sprayed onto the leaves of 4-week-old O. minuta seedlings, as reported previously (Shim et al, 2004). At least 10 larvae of the BPH (Nilaparvata lugens) Korean biotype1 were placed on each plant (Cho et al, 2005).…”

Physiological and Molecular Alterations Promoted by Schizotetranychus oryzae Mite Infestation in Rice Leaves

“…A NifU-like gene was up-regulated when wild rice ( Oryza minuta ) was exposed to Magnaporthe grisea infection 105 . Villins are actin modulating proteins 106 .…”