Identification of downy mildew resistance genes <i>Rpv10</i> and <i>Rpv3</i> by DNA-marker analysis in a Russian grapevine germplasm collection

Ilnitskayaa, E.; Токмаков, С. В.; Makarkina, Marina Victorovna; Супрун, И. И.

doi:10.17660/actahortic.2019.1248.19

Cited by 9 publications

(11 citation statements)

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“…We have already started research for searching gene Rpv3 donors and some of them are published [13][14][15]. A large work in this direction was done by Di Gaspero and colleagues: DNA-marker analysis of the Rpv3 locus in 580 genotypes of various origins [10].…”

Section: Resultsmentioning

confidence: 99%

DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

et al. 2020

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DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes Rpv3 (inherited from North American grape species) and Rpv12 (derived from V. amurensis) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the Rpv3 gene and 8 varieties for the presence of the Rpv12 gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the Rpv3299-279 allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the Rpv12 gene in the genotypes. One table grape genotype was identified with Rpv3 and Rpv12.

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Section: Resultsmentioning

confidence: 99%

DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

et al. 2020

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“…Эффективным для создания высокоустойчивых форм будет гибридизация сортов, несущих ген Rpv3 с сортами, унаследовавшими гены устойчивости к милдью Rpv10 и Rpv12 от V. amurensis (Foria et al, 2019). Так например, Rpv10 детектирован в ДНК бессемянного сорта Коринка русская (Ilnitskaya et al, 2019b). Анализ происхождения сорта Кишмиш запорожский говорит о присутствии V. amurensis в его родословной, и есть вероятность, что данный сорт также может обладать генами устойчивости амурского винограда к милдью от родительской формы -сорта Виктория ((V. vinifera x V. amurensis) x СВ 12-304).…”

Section: таблица результаты днк-маркерного анализа сортов виноградаunclassified

DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

Ильницкая

Makarkina

Токмаков

2020

Biotehnologiâ i selekciâ rastenij

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Table grapes are a valuable dietary product. Seedless grapes are in high demand among consumers. For this reason, the breeding of seedless varieties is one of the popular trends in modern viticulture, along with the production of environmentally friendly products. Downy mildew (Plasmopara viticola (Berk. & M.A. Curtis) Berl. & De Toni) is one of the most common fungal diseases of the grapevine. Most downy mildew resistant grape accessions belong to North American species like Vitis aestivalis Michx., V. berlandieri Planch., V. cinerea (Engelm. ex A. Gray) Engelm. ex Millard, V. riparia Michx., V. rupestris Scheele, etc. The search for donors of resistance genes is an urgent task. Rpv3 is one of the most significant resistance genes from a number of North American grape varieties. The aim of this work is to identify the downy mildew resistance gene Rpv3 in seedless grape varieties by means of DNA-marker analysis. The grape varieties with rudimentary development of seed in berries and with North American species in the pedigree were chosen as the object of the study. The varieties “Dunavski lazur” and “Seyve Villard 12-375” with reference alleles were used as the positive control, while V. vinifera L. was used as the negative control. UDV305 and UDV737 DNA-markers were used in this study to identify the allelic type of the Rpv3 gene. The work was performed using the polymerase chain reaction. The reaction products were separated by capillary electrophoresis using the ABI Prism 3130 automatic genetic analyzer. Evaluation of the results was done using the GeneMapper and PeakScanner software. Functional alleles of the downy mildew resistance gene Rpv3 were revealed in grape varieties “Kishmish zaporozhskiy”, “Lady Patricia”, “Remaily seedless”, “Pamyati Smirnova” and “Shayan”. Rpv3299-279, one of the seven known haplotypes, was identified in all the varieties. The pedigree analysis of the studied varieties indicated that the parental forms – “Seyve Villard” and “Seibel” hybrids – are presumably the donors of the gene. Grape accessions with the identified Rpv3 gene can be used in seedless varieties breeding as donors of resistance to downy mildew.

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“…In the literature we can find scientific information concerning Crimean, Dagestan, Don and other aboriginal forms of grapes [7][8][9][10][11]. But information on agrestic or autochthonous Kuban forms is practically absent.…”

Section: Introductionmentioning

confidence: 99%

New studies of wild-growing grapes of Kuban: ecology, morphology, variability

Gorbunov

Luk'yanov

2021

E3S Web Conf.

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The purpose of this scientific work was to find and study the variety of wild grapes as future promising sources of selection and valuable traits on the example of the territory of the natural reserve «Red Forest”. Analysis of modern scientific literature on the study of Vitaceae Juss. family biodiversity showed that there is practically no scientific information on agrestic Kuban forms and autochthones of grapes. This article represents the beginning of a new large research work on the study of wild Kuban grapes under study in the territory of Krasnodar Krai, in particular, in the state natural reserve “Red Forest”. The following methods were used in the work: route reconnaissance (during expeditions), traditional geobotanical and ampelographic, analytical. 5 isolated populations of agrestic grapes have been found on the territory of the “Red Forest” nature reserve. For the first time, these forms were studied and described by the ecological and geographical growth conditions and morphological traits of vegetative and generative spheres of grape plants. Significant variability of some indicators has been revealed. The presence of damage by pests and diseases was not visually detected. Hypothetically, these isolated grape populations may belong to Vitis vinifera ssp. Silvestris Gmel. or its variation var. Tipica Negr. (wild forest grapes). Samples were taken for further genetic analysis. This research was carried out within the framework of the RFFR grant project (contract No.19–416–230025).

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Identification of downy mildew resistance genes Rpv10 and Rpv3 by DNA-marker analysis in a Russian grapevine germplasm collection

Cited by 9 publications

References 6 publications

DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties

DNA marker identification of Rpv3 downy mildew resistance gene in seedless grape varieties

New studies of wild-growing grapes of Kuban: ecology, morphology, variability

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