Identification of Differentially Expressed Serum Proteins in Infectious Purpura Fulminans

Abstract

Purpura fulminans (PF) is a life-threatening hemorrhagic condition. Because of the rarity and randomness of the disease, no improvement in treatment has been made for a long time. In this study, we assessed the serum proteome response to PF by comparing serum proteins between healthy controls and PF patient. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) approach was used after depleting 6 abundant proteins of serum. In total, 262 proteins were confidently identified with 2 unique peptides, and 38 proteins were identified significantly up- (≥2) or downregulated (≤0.5) based on spectral counting ratios (SpCPF/N). In the 38 proteins with significant abundance changes, 11 proteins were previously known to be associated with burn or sepsis response, but 27 potentially novel proteins may be specifically associated with PF process. Two differentially expressed proteins, alpha-1-antitrypsin (SERPINA1) and alpha-2 antiplasmin (SERPINF2), were validated by Western blot. This is the first study where PF patient and healthy controls are compared in a proteomic study to elucidate proteins involved in the response to PF. This study provides an initial basis for future studies of PF, and the differentially expressed proteins might provide new therapeutic targets to decrease the mortality of PF.

Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis

“…In our previous study, proteomics analysis was applied to compare the changes in serum proteins in patients with sepsis followed by purpura fulminans (PF) to those in healthy individuals [8]. Consistent with previous studies, pigment epitheliumderived factor (PEDF) was found to be significantly increased in septic patients [9].…”

“…The elevated PEDF in diabetic patients is found to be associated with microvascular complications and poor vascular health [32]. Recently, PEDF was found to be significantly elevated in patients with sepsis [8,9], although its role in vascular permeability remains elusive. The conclusions from previous studies suggest that PEDF exerts several important but contradictory influences on the VE barrier [11][12][13].…”

Combination of HBA1, TTR, and SERPINF2 in plasma defines phenotype correlated with severe burn outcome

“… 31 Serum mass spectrometry reports of purpura fulminans showed significantly decreased expression of SERPINF2, suggesting its association with uncontrolled fibrinolysis and thrombosis. 32 With regard to burns, a pediatric report showed that alpha-2-antiplasmin is decreased after burns and contributes to an increase in total proteolytic activity. 33 In the present study, we newly showed that the degree of decrease in SERPINF2 is related to mortality and correlated with measures of clinical severity such as the SOFA score.…”

Following Up of Surgical Treated Human Liver Cystic Echinococcosis: A Proteomics Approach

“…This glycoprotein is essentially synthesized in the liver and secreted into the bloodstream ( 32 ). It is an effective inhibitor of neutrophil elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator ( 33 ). In this study, we observed that the expression of SERPINA1 was suppressed after the surgery.…”