Identification of differentially expressed genes in response to mercury I and II stress in Trichoderma harzianum

Puglisi, Ivana; Faedda, Roberto; Sanzaro, Vincenzo; Piero, Angela Roberta Lo; Petrone, Goffredo; Cacciola, S. O.

doi:10.1016/j.gene.2012.06.091

Cited by 48 publications

(33 citation statements)

References 26 publications

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“…The values presented as the means ± standard deviations at the end of the experiment (after 90 days of fungi growth). transformation, precipitation and production of cell wall metal-binding proteins such as metallothioneins, as well as cysteine-rich proteins called hydrophobins, extracellular polymeric substances (EPS), and pigments (Gadd, 2007;Pócsi, 2011;Puglisi et al, 2012). Overall, fungi could interact with metals by several different physico-chemical and biological mechanisms leading to their bioaccumulation, biosorption, micro-precipitation and chemical transformation.…”

Section: Discussionmentioning

confidence: 99%

Antifungal properties of silver nanoparticles against indoor mould growth

Ogar

Tylko

Turnau

2015

Science of The Total Environment

104

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Section: Discussionmentioning

confidence: 99%

Antifungal properties of silver nanoparticles against indoor mould growth

Ogar

Tylko

Turnau

2015

Science of The Total Environment

104

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“…Alam et al [36] optimized the process of cellulase production by Trichoderma reesei through the bioconversion of the liquid sewage sludge, finding maximal activity at pH 5.5, thus corroborating Qin et al [37], Zang et al [38], Bendig and Weuster-Botz [39] and Puglisi et al [40]. Table 3 and Figure 1 show the same behavior for the four tested strains during the production of cellulolytic complex, with enzyme production occurring from 22 days of fermentation to reach maximum yields at day 29.…”

Section: Resultsmentioning

confidence: 69%

Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)

Onofre¹,

Bonfante²,

Santos³

et al. 2014

AiM

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Cellulases are enzymes responsible for the degradation of cellulose, the major compound in plant cells. Cellulose is a polysaccharide composed of several glucose units linked together by chemical bonds. Cellulases, such as endoglucanases, beta-glucosidase and exoglucanases, break the chemical bonds between the glucose units. Fungi, including the endophytic species, can be great cellulase producers. This study aimed to evaluate cellulase production by four endophytic strains of Trichoderma reesei in semi-solid media containing sugarcane bagasse, supplemented or not with salts. Two fermentations were carried out for 43 days. Samples were taken every seven days to obtain production peaks. The enzymes were characterized by their optimum pH and temperature of activity and stability upon incubation in the presence of ions, pH and temperature variations. The results showed that the endophytic strains FB1, FB2, FB3 and FB4 of Trichoderma reesei produce cellulases in a sugarcane bagasse medium, supplemented or not with salts, at pH 5.5 and 30˚C. The supplemented medium proved to be more appropriate to induce cellulase production after 29 days of fermentation, with FB4 having the best yield: 16.32 ± 2.65 IU/gram of fermented substrate.

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“…Furthermore, we expected upregulation in qid74 because it aids attachment to hydrophobic surfaces [44], a first step in modifying the surface [45, 60]. Additionally, qid74 and has been shown to be involved in pollutant coping mechanisms [47, 61], yet none of these genes were significantly upregulated at the times sampled and therefore we cannot draw any meaningful conclusion regarding mechanisms for this study. In T. harzianum , there are 73 genes that could potentially be stimulated by chitin [28, 29] and any of those genes could lead to anthracene removal.…”

Section: Resultsmentioning

confidence: 98%

“…For instance, filamentous fungi have evolved mechanisms of attachment to hydrophobic surfaces (via hydrophobins) in order to parasitize plants or other fungi [43–45]. These same mechanisms have also been shown to be active when challenged with non-living hydrophobic surfaces such as Teflon and to be upregulated during heavy-metal bioremediation [45–47]. …”

Section: Resultsmentioning

confidence: 99%

Evaluating the mycostimulation potential of select carbon amendments for the degradation of a model PAH by an ascomycete strain enriched from a superfund site

et al. 2018

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Although ecological flexibility has been well documented in fungi, it remains unclear how this flexibility can be exploited for pollutant degradation, especially in the Ascomycota phylum. In this work, we assess three mycostimulation amendments for their ability to induce degradation in Trichoderma harzanium, a model fungus previously isolated from a Superfund site contaminated with polycyclic aromatic hydrocarbons. The amendments used in the present study were selected based on the documented ecological roles of ascomycetes. Chitin was selected to simulate the parasitic ecological role while cellulose and wood were selected to mimic bulk soil and wood saprobic conditions, respectively. Each amendment was tested in liquid basal medium in 0.1 and 1% (w/v) suspensions. Both chitin and cellulose amendments were shown to promote anthracene degradation in T. harzanium with the 0.1% chitin amendment resulting in over 90% removal of anthracene. None of the targets monitored for gene expression were found to be upregulated suggesting alternate pathways may be used in T. harzanium. Overall, our data suggest that mycostimulation amendments can be improved by understanding the ecological roles of indigenous fungi. However, further research is needed to better estimate specific amendment requirements for a broader group of target fungi and follow up studies are needed to determine whether the trends observed herein translate to more realistic soil systems.

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Identification of differentially expressed genes in response to mercury I and II stress in Trichoderma harzianum

Cited by 48 publications

References 26 publications

Antifungal properties of silver nanoparticles against indoor mould growth

Antifungal properties of silver nanoparticles against indoor mould growth

Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)

Evaluating the mycostimulation potential of select carbon amendments for the degradation of a model PAH by an ascomycete strain enriched from a superfund site

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Identification of differentially expressed genes in response to mercury I and II stress in Trichoderma harzianum

Cited by 48 publications

References 26 publications

Antifungal properties of silver nanoparticles against indoor mould growth

Antifungal properties of silver nanoparticles against indoor mould growth

Cellulase Production by Endophytic Strains of &lt;i&gt;Trichoderma reesei&lt;/i&gt; from &lt;i&gt;Baccharis dracunculifolia&lt;/i&gt; D. C. (Asteraceae)

Evaluating the mycostimulation potential of select carbon amendments for the degradation of a model PAH by an ascomycete strain enriched from a superfund site

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Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)