Identification of CX3CR1+ mononuclear phagocyte subsets involved in HIV-1 and SIV colorectal transmission

Women for science and science for women: Gaps, challenges and opportunities towards optimizing pre-exposure prophylaxis for HIV-1 prevention

“…Whether other immune cells contribute to the loss of the EB during HIV infection, has been less explored. Recently, a role for intestinal CX3CR1+ mononuclear phagocytes and NKG2a/c+CD8+ T has been proposed in the protection of the gut barrier function to avoid intestinal inflammation during SIV infection ( 230 , 231 ). While those cells are implicated in the EB protection also in the FGT is unknown.…”

“…In addition, antigen presenting cells (APCs), including DCs, LCs and macrophages, are strategically located to be initial target of the virus and to transfer infection to T lymphocytes ( 219 , 237 – 239 ). We are just starting to have a more precise idea of the different APC subsets present in the genital mucosa and the specific role each of those subsets play in both HIV infection and anti-viral immune responses ( 230 , 240 – 242 ). The distribution of immune cells as well as their ability to respond to a stimulus differs according to the female genital tract compartment and are distinct from systemic immune cells ( 243 ).…”

Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

“…Further information on expected frequencies and phenotypic marker expression, such as CD103, CX3CR1 and CD14 by DCs and Mφs subsets, can be found on a previously published research article that have used this protocol ( Cavarelli et al., 2022 ). By using the same digestion protocol and an antibody panel specifically designed for lymphocytes identification (not detailed here), T cell subsets composition of the macaque colon was previously described ( Cavarelli et al., 2021 ).…”

“…The protocol is repeatable and scalable for downstream applications. For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022) .…”

“…For complete details on the use and execution of this protocol, please refer to Cavarelli et al. (2022) .…”