Isolation and phenotypic characterization of human and nonhuman primate intestinal lamina propria mononuclear cells

Immunogenicity and safety of an Entamoeba histolytica adjuvanted protein vaccine candidate (LecA+GLA-3M-052 liposomes) in rhesus macaques

“…Mononuclear cell suspensions were passed through a 70-μM strainer followed by an RBC lysis step. Lamina propria mononucleated cells (LPMCs) were isolated from the cecum as previously described 26 (see Supplementary Methods). All cells were seeded accordingly in 96-well ELISpot plates as above.…”